Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances

Detalhes bibliográficos
Autor(a) principal: Klein, W.
Data de Publicação: 2012
Outros Autores: Vidal, D., Fortunato, G., Cortizo, L., Vasconcelos, J., Santos, R. Ribeiro dos, Soares, M. G. A, Macambira, Simone Garcia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15541
Resumo: Texto completo: acesso restrito. p. 128–136
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spelling Klein, W.Vidal, D.Fortunato, G.Cortizo, L.Vasconcelos, J.Santos, R. Ribeiro dosSoares, M. G. AMacambira, Simone GarciaKlein, W.Vidal, D.Fortunato, G.Cortizo, L.Vasconcelos, J.Santos, R. Ribeiro dosSoares, M. G. AMacambira, Simone Garcia2014-08-11T18:51:41Z20120873-2159http://repositorio.ufba.br/ri/handle/ri/15541v. 18, n. 3Texto completo: acesso restrito. p. 128–136Several experimental studies of pulmonary emphysema using animal models have been described in the literature. However, only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema. Additionally, functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema. The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment. This was evaluated by measuring exercise capacity in C57Bl/6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema. Thirty six mice underwent ergometric tests before and 28 days after elastase administration. Pancreatic porcine elastase solution was administered by tracheal puncture, which resulted in a significantly decreased exercise capacity, shown by a shorter distance run (−30.5%) and a lower mean velocity (−15%), as well as in failure to increase the elimination of carbon dioxide. The mean linear intercept increased significantly by 50% in tracheal elastase administration. In conclusion, application of elastase by tracheal function in C57Bl/6 induces emphysema, as validated by morphometric analyses, and resulted in a significantly lower exercise capacity, while resulting in a low mortality rate.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-11T14:27:38Z No. of bitstreams: 1 W. 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Klein.pdf: 364166 bytes, checksum: dc8d2f0955fdc78540b39de88b7f6085 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1016/j.rppneu.2011.12.007reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPulmonary emphysemaErgometric testPorcine elastaseC57Bl/6Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbancesRevista Portuguesa de Pneumologiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessporLICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15541/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52ORIGINALW. Klein.pdfW. Klein.pdfapplication/pdf364166https://repositorio.ufba.br/bitstream/ri/15541/1/W.%20Klein.pdfdc8d2f0955fdc78540b39de88b7f6085MD51TEXTW. Klein.pdf.txtW. 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dc.title.pt_BR.fl_str_mv Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
dc.title.alternative.pt_BR.fl_str_mv Revista Portuguesa de Pneumologia
title Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
spellingShingle Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
Klein, W.
Pulmonary emphysema
Ergometric test
Porcine elastase
C57Bl/6
title_short Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
title_full Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
title_fullStr Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
title_full_unstemmed Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
title_sort Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
author Klein, W.
author_facet Klein, W.
Vidal, D.
Fortunato, G.
Cortizo, L.
Vasconcelos, J.
Santos, R. Ribeiro dos
Soares, M. G. A
Macambira, Simone Garcia
author_role author
author2 Vidal, D.
Fortunato, G.
Cortizo, L.
Vasconcelos, J.
Santos, R. Ribeiro dos
Soares, M. G. A
Macambira, Simone Garcia
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Klein, W.
Vidal, D.
Fortunato, G.
Cortizo, L.
Vasconcelos, J.
Santos, R. Ribeiro dos
Soares, M. G. A
Macambira, Simone Garcia
Klein, W.
Vidal, D.
Fortunato, G.
Cortizo, L.
Vasconcelos, J.
Santos, R. Ribeiro dos
Soares, M. G. A
Macambira, Simone Garcia
dc.subject.por.fl_str_mv Pulmonary emphysema
Ergometric test
Porcine elastase
C57Bl/6
topic Pulmonary emphysema
Ergometric test
Porcine elastase
C57Bl/6
description Texto completo: acesso restrito. p. 128–136
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-08-11T18:51:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15541
dc.identifier.issn.none.fl_str_mv 0873-2159
dc.identifier.number.pt_BR.fl_str_mv v. 18, n. 3
identifier_str_mv 0873-2159
v. 18, n. 3
url http://repositorio.ufba.br/ri/handle/ri/15541
dc.language.iso.fl_str_mv por
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