Influence of laser photobiomodulation upon connective tissue remodeling during wound healing

Detalhes bibliográficos
Autor(a) principal: Medrado, Alena Ribeiro Alves Peixoto
Data de Publicação: 2008
Outros Autores: Soares, Ana Prates, Santos, Elisângela T., Reis, Sílvia Regina A., Andrade, Zilton de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6041
Resumo: Acesso restrito: Texto completo. p. 144-152
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spelling Medrado, Alena Ribeiro Alves PeixotoSoares, Ana PratesSantos, Elisângela T.Reis, Sílvia Regina A.Andrade, Zilton de AraújoMedrado, Alena Ribeiro Alves PeixotoSoares, Ana PratesSantos, Elisângela T.Reis, Sílvia Regina A.Andrade, Zilton de Araújo2012-06-01T12:29:36Z20081011-1344http://www.repositorio.ufba.br/ri/handle/ri/6041v. 92, n. 3Acesso restrito: Texto completo. p. 144-152The modulation of collagen fibers during experimental skin wound healing was studied in 112 Wistar rats submitted to laser photobiomodulation treatment. A standardized 8 mm-diameter wound was made on the dorsal skin of all animals. In half of them, 0.2 ml of a silica suspension was injected along the border of the wound in order to enhance collagen deposition and facilitate observation. The others received saline as vehicle. The treatment was carried out by means of laser rays from an aluminum–gallium arsenide diode semiconductor with 9 mW applied every other day (total dose = 4 J/cm2) on the borders of the wound. Tissue sections obtained from four experimental groups representing sham-irradiated animals, laser, silica and the association of both, were studied after 3, 7, 10, 15, 20, 30 and 60 days from the laser application. The wounded skin area was surgically removed and submitted to histological, immunohistochemical, ultrastructural, and immunofluorescent studies. Besides the degree and arrangement of collagen fibers and of their isotypes, the degree of edema, the presence of several cell types especially pericytes and myofibroblasts, were described and measured. The observation of Sirius-red stained slides under polarized microscopy revealed to be of great help during the morphological analysis of the collagen tissue dynamic changes. It was demonstrated that laser application was responsible for edema regression and a diminution in the number of inflammatory cells (p < 0.05). An evident increase in the number of actin-positive cells was observed in the laser-treated wounds. Collagen deposition was less than expected in silica-treated wounds, and laser treatment contributed to its better differentiation and modulation in all irradiated groups. Thus, laser photobiomodulation was able to induce several modifications during the cutaneous healing process, especially in favoring newly-formed collagen fibers to be better organized and compactedly disposed.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-06-01T12:29:36Z No. of bitstreams: 1 __ac.els-cdn.com_S101113...be492a78df2092df7f8a794f2d21f.pdf: 975996 bytes, checksum: 397bfba0f482d83e2c9294b36211a8b2 (MD5)Made available in DSpace on 2012-06-01T12:29:36Z (GMT). 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dc.title.pt_BR.fl_str_mv Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
dc.title.alternative.pt_BR.fl_str_mv Journal of Photochemistry and Photobiology B-Biology
title Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
spellingShingle Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
Medrado, Alena Ribeiro Alves Peixoto
Low level laser
Collagen
Silica
Fibrosis
title_short Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
title_full Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
title_fullStr Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
title_full_unstemmed Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
title_sort Influence of laser photobiomodulation upon connective tissue remodeling during wound healing
author Medrado, Alena Ribeiro Alves Peixoto
author_facet Medrado, Alena Ribeiro Alves Peixoto
Soares, Ana Prates
Santos, Elisângela T.
Reis, Sílvia Regina A.
Andrade, Zilton de Araújo
author_role author
author2 Soares, Ana Prates
Santos, Elisângela T.
Reis, Sílvia Regina A.
Andrade, Zilton de Araújo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Medrado, Alena Ribeiro Alves Peixoto
Soares, Ana Prates
Santos, Elisângela T.
Reis, Sílvia Regina A.
Andrade, Zilton de Araújo
Medrado, Alena Ribeiro Alves Peixoto
Soares, Ana Prates
Santos, Elisângela T.
Reis, Sílvia Regina A.
Andrade, Zilton de Araújo
dc.subject.por.fl_str_mv Low level laser
Collagen
Silica
Fibrosis
topic Low level laser
Collagen
Silica
Fibrosis
description Acesso restrito: Texto completo. p. 144-152
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-06-01T12:29:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6041
dc.identifier.issn.none.fl_str_mv 1011-1344
dc.identifier.number.pt_BR.fl_str_mv v. 92, n. 3
identifier_str_mv 1011-1344
v. 92, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/6041
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.jphotobiol.2008.05.008
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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