An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

Detalhes bibliográficos
Autor(a) principal: Roque, Milton Ricardo de Abreu
Data de Publicação: 2012
Outros Autores: Paulo, Elinalva Maciel, Vasconcelos, Murilo Pinho, Oliveira, Ivelise Santiago, Affe, Helen Michelle de Jesus, Nascimento, Rosely, Melo, Itamar Soares de, Assis, Sandra Aparecida de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14888
Resumo: p. 710-714
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spelling Roque, Milton Ricardo de AbreuPaulo, Elinalva MacielVasconcelos, Murilo PinhoOliveira, Ivelise SantiagoAffe, Helen Michelle de JesusNascimento, RoselyMelo, Itamar Soares deAssis, Sandra Aparecida de2014-05-01T13:00:09Z2014-05-01T13:00:09Z20120101-206http://repositorio.ufba.br/ri/handle/ri/14888v. 32, n. 4p. 710-714The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 ºC. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-29T14:00:51Z No. of bitstreams: 1 Milton Ricardo de Abreu Roque.pdf: 3240469 bytes, checksum: e0aece3686bd76f91c3d9159dd78b6fb (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-05-01T13:00:09Z (GMT) No. of bitstreams: 1 Milton Ricardo de Abreu Roque.pdf: 3240469 bytes, checksum: e0aece3686bd76f91c3d9159dd78b6fb (MD5)Made available in DSpace on 2014-05-01T13:00:09Z (GMT). No. of bitstreams: 1 Milton Ricardo de Abreu Roque.pdf: 3240469 bytes, checksum: e0aece3686bd76f91c3d9159dd78b6fb (MD5) Previous issue date: 2012http://dx.doi.org/10.1590/S0101-20612012005000094reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAScreeningExopolysaccharidesLactic acid bacteriaTriagemExopolissacarídeosBactérias lácticasAn alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmationCiência e Tecnologia de Alimentosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengLICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14888/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52ORIGINALMilton Ricardo de Abreu Roque.pdfMilton Ricardo de Abreu Roque.pdfapplication/pdf3240469https://repositorio.ufba.br/bitstream/ri/14888/1/Milton%20Ricardo%20de%20Abreu%20Roque.pdfe0aece3686bd76f91c3d9159dd78b6fbMD51TEXTMilton Ricardo de Abreu Roque.pdf.txtMilton Ricardo de Abreu Roque.pdf.txtExtracted texttext/plain24623https://repositorio.ufba.br/bitstream/ri/14888/3/Milton%20Ricardo%20de%20Abreu%20Roque.pdf.txt33f8442cd0f9aa3cbb2387f33c2f6103MD53ri/148882022-03-09 20:30:22.397oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-03-09T23:30:22Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
dc.title.alternative.pt_BR.fl_str_mv Ciência e Tecnologia de Alimentos
title An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
spellingShingle An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
Roque, Milton Ricardo de Abreu
Screening
Exopolysaccharides
Lactic acid bacteria
Triagem
Exopolissacarídeos
Bactérias lácticas
title_short An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
title_full An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
title_fullStr An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
title_full_unstemmed An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
title_sort An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation
author Roque, Milton Ricardo de Abreu
author_facet Roque, Milton Ricardo de Abreu
Paulo, Elinalva Maciel
Vasconcelos, Murilo Pinho
Oliveira, Ivelise Santiago
Affe, Helen Michelle de Jesus
Nascimento, Rosely
Melo, Itamar Soares de
Assis, Sandra Aparecida de
author_role author
author2 Paulo, Elinalva Maciel
Vasconcelos, Murilo Pinho
Oliveira, Ivelise Santiago
Affe, Helen Michelle de Jesus
Nascimento, Rosely
Melo, Itamar Soares de
Assis, Sandra Aparecida de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Roque, Milton Ricardo de Abreu
Paulo, Elinalva Maciel
Vasconcelos, Murilo Pinho
Oliveira, Ivelise Santiago
Affe, Helen Michelle de Jesus
Nascimento, Rosely
Melo, Itamar Soares de
Assis, Sandra Aparecida de
dc.subject.por.fl_str_mv Screening
Exopolysaccharides
Lactic acid bacteria
Triagem
Exopolissacarídeos
Bactérias lácticas
topic Screening
Exopolysaccharides
Lactic acid bacteria
Triagem
Exopolissacarídeos
Bactérias lácticas
description p. 710-714
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-05-01T13:00:09Z
dc.date.available.fl_str_mv 2014-05-01T13:00:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14888
dc.identifier.issn.none.fl_str_mv 0101-206
dc.identifier.number.pt_BR.fl_str_mv v. 32, n. 4
identifier_str_mv 0101-206
v. 32, n. 4
url http://repositorio.ufba.br/ri/handle/ri/14888
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.country.fl_str_mv Brasil
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0101-20612012005000094
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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