An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis

Detalhes bibliográficos
Autor(a) principal: Chagas Júnior, Adenizar Delgado das
Data de Publicação: 2009
Outros Autores: McBride, Alan J. A., Athanazio, Daniel Abensur, Figueira, Cláudio P., Medeiros, Marco A., Reis, Mitermayer Galvão dos, Ko, Albert I., McBride, Flávia W. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/12965
Resumo: Texto completo: acesso restrito. p.1632-1637
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spelling Chagas Júnior, Adenizar Delgado dasMcBride, Alan J. A.Athanazio, Daniel AbensurFigueira, Cláudio P.Medeiros, Marco A.Reis, Mitermayer Galvão dosKo, Albert I.McBride, Flávia W. C.Chagas Júnior, Adenizar Delgado dasMcBride, Alan J. A.Athanazio, Daniel AbensurFigueira, Cláudio P.Medeiros, Marco A.Reis, Mitermayer Galvão dosKo, Albert I.McBride, Flávia W. C.2013-09-17T17:28:49Z20090022-2615http://www.repositorio.ufba.br/ri/handle/ri/12965v. 58, n. 12Texto completo: acesso restrito. p.1632-1637In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (κ=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (κ=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-17T14:33:03Z No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5)Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-09-17T17:28:49Z (GMT) No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5)Made available in DSpace on 2013-09-17T17:28:49Z (GMT). No. of bitstreams: 1 0.1099jmm.0.014050-0.pdf: 220400 bytes, checksum: a7d90c3e814de07b78e40307f7d81071 (MD5) Previous issue date: 2010http://dx.doi.org/10.1099/jmm.0.014050-0reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAn imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosisJournal of Medical Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12965/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL0.1099jmm.0.014050-0.pdf0.1099jmm.0.014050-0.pdfapplication/pdf220400https://repositorio.ufba.br/bitstream/ri/12965/1/0.1099jmm.0.014050-0.pdfa7d90c3e814de07b78e40307f7d81071MD51TEXT0.1099jmm.0.014050-0.pdf.txt0.1099jmm.0.014050-0.pdf.txtExtracted texttext/plain29181https://repositorio.ufba.br/bitstream/ri/12965/3/0.1099jmm.0.014050-0.pdf.txt4d41994e0a6a92ded05341b527e71ddfMD53ri/129652022-10-06 10:33:10.851oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-06T13:33:10Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
dc.title.alternative.pt_BR.fl_str_mv Journal of Medical Microbiology
title An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
spellingShingle An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
Chagas Júnior, Adenizar Delgado das
title_short An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
title_full An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
title_fullStr An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
title_full_unstemmed An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
title_sort An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
author Chagas Júnior, Adenizar Delgado das
author_facet Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Athanazio, Daniel Abensur
Figueira, Cláudio P.
Medeiros, Marco A.
Reis, Mitermayer Galvão dos
Ko, Albert I.
McBride, Flávia W. C.
author_role author
author2 McBride, Alan J. A.
Athanazio, Daniel Abensur
Figueira, Cláudio P.
Medeiros, Marco A.
Reis, Mitermayer Galvão dos
Ko, Albert I.
McBride, Flávia W. C.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Athanazio, Daniel Abensur
Figueira, Cláudio P.
Medeiros, Marco A.
Reis, Mitermayer Galvão dos
Ko, Albert I.
McBride, Flávia W. C.
Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Athanazio, Daniel Abensur
Figueira, Cláudio P.
Medeiros, Marco A.
Reis, Mitermayer Galvão dos
Ko, Albert I.
McBride, Flávia W. C.
description Texto completo: acesso restrito. p.1632-1637
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-09-17T17:28:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12965
dc.identifier.issn.none.fl_str_mv 0022-2615
dc.identifier.number.pt_BR.fl_str_mv v. 58, n. 12
identifier_str_mv 0022-2615
v. 58, n. 12
url http://www.repositorio.ufba.br/ri/handle/ri/12965
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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