Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR

Detalhes bibliográficos
Autor(a) principal: Chagas Júnior, Adenizar Delgado das
Data de Publicação: 2012
Outros Autores: Silva, Caroline L. R. da, Soares, Luciane Marieta, Santos, Cleiton S., Silva, Carlos D. C. M., Athanazio, Daniel Abensur, Reis, Mitermayer Galvão dos, McBride, Flávia W. Cruz, McBride, Alan J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15666
Resumo: p. 1-5
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spelling Chagas Júnior, Adenizar Delgado dasSilva, Caroline L. R. daSoares, Luciane MarietaSantos, Cleiton S.Silva, Carlos D. C. M.Athanazio, Daniel AbensurReis, Mitermayer Galvão dosMcBride, Flávia W. CruzMcBride, Alan J. A.2014-08-21T19:29:23Z2014-08-21T19:29:23Z20121932-6203http://repositorio.ufba.br/ri/handle/ri/15666v. 7, n. 2p. 1-5A major limitation in the clinical management and experimental research of leptospirosis is the poor performance of the available methods for the direct detection of leptospires. In this study, we compared real-time PCR (qPCR), targeting the lipL32 gene, with the immunofluorescent imprint method (IM) for the detection and quantification of leptospires in kidney samples from the rat and hamster experimental models of leptospirosis. Using a virulent strain of Leptospira interrogans serovar Copenhageni, a chronic infection was established in the rat model, which were euthanized 28 days post-infection, while the hamster model simulated an acute infection and the hamsters were euthanized eight days after inoculation. Leptospires in the kidney samples were detected using culture isolation, qPCR and the IM, and quantified using qPCR and the IM. In both the acute and chronic infection models, the correlation between quantification by qPCR and the IM was found to be positive and statistically significant (P<0.05). Therefore, this study demonstrates that the IM is a viable alternative for not only the detection but also the quantification of leptospires, particularly when the use of qPCR is not feasible.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-20T14:10:57Z No. of bitstreams: 1 Adenizar D. Chagas Junior.pdf: 178080 bytes, checksum: b2561b9825462940327c01c8b52fc3cd (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-08-21T19:29:23Z (GMT) No. of bitstreams: 1 Adenizar D. Chagas Junior.pdf: 178080 bytes, checksum: b2561b9825462940327c01c8b52fc3cd (MD5)Made available in DSpace on 2014-08-21T19:29:23Z (GMT). No. of bitstreams: 1 Adenizar D. Chagas Junior.pdf: 178080 bytes, checksum: b2561b9825462940327c01c8b52fc3cd (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1371/journal.pone.0032712reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBADetection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCRPLoS ONEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengLICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15666/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52ORIGINALAdenizar D. Chagas Junior.pdfAdenizar D. Chagas Junior.pdfapplication/pdf178080https://repositorio.ufba.br/bitstream/ri/15666/1/Adenizar%20D.%20Chagas%20Junior.pdfb2561b9825462940327c01c8b52fc3cdMD51TEXTAdenizar D. Chagas Junior.pdf.txtAdenizar D. 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dc.title.pt_BR.fl_str_mv Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
dc.title.alternative.pt_BR.fl_str_mv PLoS ONE
title Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
spellingShingle Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
Chagas Júnior, Adenizar Delgado das
title_short Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
title_full Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
title_fullStr Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
title_full_unstemmed Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
title_sort Detection and Quantification of Leptospira interrogans in Hamster and Rat Kidney Samples: Immunofluorescent Imprints versus Real-time PCR
author Chagas Júnior, Adenizar Delgado das
author_facet Chagas Júnior, Adenizar Delgado das
Silva, Caroline L. R. da
Soares, Luciane Marieta
Santos, Cleiton S.
Silva, Carlos D. C. M.
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia W. Cruz
McBride, Alan J. A.
author_role author
author2 Silva, Caroline L. R. da
Soares, Luciane Marieta
Santos, Cleiton S.
Silva, Carlos D. C. M.
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia W. Cruz
McBride, Alan J. A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chagas Júnior, Adenizar Delgado das
Silva, Caroline L. R. da
Soares, Luciane Marieta
Santos, Cleiton S.
Silva, Carlos D. C. M.
Athanazio, Daniel Abensur
Reis, Mitermayer Galvão dos
McBride, Flávia W. Cruz
McBride, Alan J. A.
description p. 1-5
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-08-21T19:29:23Z
dc.date.available.fl_str_mv 2014-08-21T19:29:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15666
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.number.pt_BR.fl_str_mv v. 7, n. 2
identifier_str_mv 1932-6203
v. 7, n. 2
url http://repositorio.ufba.br/ri/handle/ri/15666
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.country.fl_str_mv Brasil
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1371/journal.pone.0032712
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