Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells

Detalhes bibliográficos
Autor(a) principal: Melo, A. R.
Data de Publicação: 2005
Outros Autores: Lasunskaia, E. B., Almeida, Cláudia Maria Costa de, Schriefer, Nicolaus Albert Borges, Kipnis, T. L., Silva, Wilmar Dias da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6483
Resumo: Trabalho completo: acesso restrito, p.511–519
id UFBA-2_d4c7b5ddf7fe0d65ee8bfa88431672c0
oai_identifier_str oai:repositorio.ufba.br:ri/6483
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Melo, A. R.Lasunskaia, E. B.Almeida, Cláudia Maria Costa deSchriefer, Nicolaus Albert BorgesKipnis, T. L.Silva, Wilmar Dias daMelo, A. R.Lasunskaia, E. B.Almeida, Cláudia Maria Costa deSchriefer, Nicolaus Albert BorgesKipnis, T. L.Silva, Wilmar Dias da2012-07-20T15:58:27Z20050300-9475http://www.repositorio.ufba.br/ri/handle/ri/6483v. 61,n. 6Trabalho completo: acesso restrito, p.511–519Localized adherence (LA) of enteropathogenic Escherichia coli (EPEC) to epithelial cells results in attaching and effacing of the surface of these cells. LA depends on the gene bfpA, which codes for the BfpA protein.Wefound that EPEC-E. coli adherence factor (EAF)(þ), expressing BfpA, significantly reduced HeLa cell viability in comparison with EPEC-EAF(–), as evaluated by the mitochondrial-dependent succinate dehydrogenase conversion of 30-[4,5,-dimethylthiazol-2yl]2,5-diphenyltetrazolium bromide (MTT) to its formazan. Apoptosis accounts for a substantial loss of the cell viability, because the cells incubated with EPEC-EAF(þ) or with cloned BfpA (data not shown), but not with EPEC-EAF(–), were positive for annexin-V binding, demonstrated chromatin condensation and nuclei fragmentation and exhibited a high level of caspase-3 activity. Because the blockade of bacterial cell-surfaceassociated BfpA by anti-BfpA immunoglobulin (Ig)Y antibody suppressed apoptotic death induced by EPEC-EAF(þ), BfpA may be the trigger for apoptosis. Both EPEC-EAF(þ) and EPEC-EAF(–), as well as recombinant BfpA (data not shown), activated nuclear factor (NF)-kB in a similar manner as analysed by the electrophoretic mobility shift assay (EMSA). EMSA supershift analysis demonstrated the presence of p65/RelA in a DNA-binding complex. In contrast to DNA binding, NF-kB-dependent reporter gene transactivation was stimulated more strongly by EPEC B171/EAF(þ), suggesting a role for this virulence factor in the regulation of transcriptional activity of NF-kB. Because suppression of NF-kB activation by BAY11-7085, a NF-kB inhibitor, neither induced apoptosis by itself nor blocked apoptosis induction by EPEC-EAF(þ), it may be suggested that apoptosis is not regulated by the NF-kB pathway in HeLa cells.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-07-20T15:58:27Z No. of bitstreams: 1 (134).pdf: 613540 bytes, checksum: 1671506279fc8e98ca005b2369e349e9 (MD5)Made available in DSpace on 2012-07-20T15:58:27Z (GMT). No. of bitstreams: 1 (134).pdf: 613540 bytes, checksum: 1671506279fc8e98ca005b2369e349e9 (MD5) Previous issue date: 2005Blackwell Publishinghttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2005.01626.x/pdfreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAExpression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host CellsScandinavian Journal of Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL(134).pdf(134).pdfapplication/pdf613540https://repositorio.ufba.br/bitstream/ri/6483/1/%28134%29.pdf1671506279fc8e98ca005b2369e349e9MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6483/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT(134).pdf.txt(134).pdf.txtExtracted texttext/plain47010https://repositorio.ufba.br/bitstream/ri/6483/3/%28134%29.pdf.txt2657a1034e3a4d6762503f41f339d145MD53ri/64832022-07-05 14:03:52.069oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:52Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
dc.title.alternative.pt_BR.fl_str_mv Scandinavian Journal of Immunology
title Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
spellingShingle Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
Melo, A. R.
title_short Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
title_full Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
title_fullStr Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
title_full_unstemmed Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
title_sort Expression of the Virulence Factor, BfpA, by Enteropathogenic Escherichia coli is Essential for Apoptosis Signalling but not for NF-kB Activation in Host Cells
author Melo, A. R.
author_facet Melo, A. R.
Lasunskaia, E. B.
Almeida, Cláudia Maria Costa de
Schriefer, Nicolaus Albert Borges
Kipnis, T. L.
Silva, Wilmar Dias da
author_role author
author2 Lasunskaia, E. B.
Almeida, Cláudia Maria Costa de
Schriefer, Nicolaus Albert Borges
Kipnis, T. L.
Silva, Wilmar Dias da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, A. R.
Lasunskaia, E. B.
Almeida, Cláudia Maria Costa de
Schriefer, Nicolaus Albert Borges
Kipnis, T. L.
Silva, Wilmar Dias da
Melo, A. R.
Lasunskaia, E. B.
Almeida, Cláudia Maria Costa de
Schriefer, Nicolaus Albert Borges
Kipnis, T. L.
Silva, Wilmar Dias da
description Trabalho completo: acesso restrito, p.511–519
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2012-07-20T15:58:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6483
dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.number.pt_BR.fl_str_mv v. 61,n. 6
identifier_str_mv 0300-9475
v. 61,n. 6
url http://www.repositorio.ufba.br/ri/handle/ri/6483
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.pt_BR.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2005.01626.x/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/6483/1/%28134%29.pdf
https://repositorio.ufba.br/bitstream/ri/6483/2/license.txt
https://repositorio.ufba.br/bitstream/ri/6483/3/%28134%29.pdf.txt
bitstream.checksum.fl_str_mv 1671506279fc8e98ca005b2369e349e9
1b89a9a0548218172d7c829f87a0eab9
2657a1034e3a4d6762503f41f339d145
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459404425560064

Registros relacionados