Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Almeida, Cláudia Maria Costa de
Data de Publicação: 2003
Outros Autores: Flores, Victor Martin Quintana, Medina-Acosta, Enrique, Schriefer, Nicolaus Albert Borges, Barral-Netto, Manoel, Silva, Wilmar Dias da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14619
Resumo: Texto completo: acesso restrito. p. 573–582
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spelling Almeida, Cláudia Maria Costa deFlores, Victor Martin QuintanaMedina-Acosta, EnriqueSchriefer, Nicolaus Albert BorgesBarral-Netto, ManoelSilva, Wilmar Dias daAlmeida, Cláudia Maria Costa deFlores, Victor Martin QuintanaMedina-Acosta, EnriqueSchriefer, Nicolaus Albert BorgesBarral-Netto, ManoelSilva, Wilmar Dias da2014-02-13T18:35:59Z20030300-9475http://repositorio.ufba.br/ri/handle/ri/14619v. 57, n. 6Texto completo: acesso restrito. p. 573–582Enteropathogenic Escherichia coli (EPEC) is a major aetiological agent of childhood diarrhoea in developing countries. The structural repeating protein A subunit, BfpA, found in the bundle-forming pilus, is one of the virulent factors for EPEC pathogenesis. Recombinant BfpA in laying hens elicited sustained and vigorous antibody production. Immunoglobulin Y (IgY) anti-BfpA antibodies were recovered from egg yolk, purified and characterized. Immunoadsorption with whole extracts of the isogenic E. coli EPEC adherence factor (EAF) strain that lacks BfpA rendered the resulting IgY preparations capable of: (a) recognizing purified or recombinant BfpA proteins in a dose-dependent fashion; (b) blocking the colonization of HeLa cells by EPEC EAF+, in vitro; (c) specifically identifying E. coli bearing EAF+; and (d) inhibiting the growth of E. coli EAF+ but not the EAF strain. IgY anti-BfpA is potentially useful as a specific, low-cost immunobiological reagent to screen human faecal specimens for the presence of EPEC.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-12-18T11:28:32Z No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T18:35:59Z (GMT) No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5)Made available in DSpace on 2014-02-13T18:35:59Z (GMT). No. of bitstreams: 1 C. M. C. De Almeida.pdf: 2417376 bytes, checksum: a9ce3051d1d24da394ead50296708427 (MD5) Previous issue date: 2003http://dx.doi.org/ 10.1046/j.1365-3083.2003.01243.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAEgg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coliScandinavian Journal of Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALC. M. C. De Almeida.pdfC. M. C. 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dc.title.pt_BR.fl_str_mv Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
dc.title.alternative.pt_BR.fl_str_mv Scandinavian Journal of Immunology
title Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
spellingShingle Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
Almeida, Cláudia Maria Costa de
title_short Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
title_full Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
title_fullStr Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
title_full_unstemmed Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
title_sort Egg Yolk Anti-BfpA Antibodies as a Tool for Recognizing and Identifying Enteropathogenic Escherichia coli
author Almeida, Cláudia Maria Costa de
author_facet Almeida, Cláudia Maria Costa de
Flores, Victor Martin Quintana
Medina-Acosta, Enrique
Schriefer, Nicolaus Albert Borges
Barral-Netto, Manoel
Silva, Wilmar Dias da
author_role author
author2 Flores, Victor Martin Quintana
Medina-Acosta, Enrique
Schriefer, Nicolaus Albert Borges
Barral-Netto, Manoel
Silva, Wilmar Dias da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Cláudia Maria Costa de
Flores, Victor Martin Quintana
Medina-Acosta, Enrique
Schriefer, Nicolaus Albert Borges
Barral-Netto, Manoel
Silva, Wilmar Dias da
Almeida, Cláudia Maria Costa de
Flores, Victor Martin Quintana
Medina-Acosta, Enrique
Schriefer, Nicolaus Albert Borges
Barral-Netto, Manoel
Silva, Wilmar Dias da
description Texto completo: acesso restrito. p. 573–582
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2014-02-13T18:35:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14619
dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.number.pt_BR.fl_str_mv v. 57, n. 6
identifier_str_mv 0300-9475
v. 57, n. 6
url http://repositorio.ufba.br/ri/handle/ri/14619
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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