Considerations on the stability of IgY antibodies antitetanus toxoid

Detalhes bibliográficos
Autor(a) principal: Guimarães, Marco Cesar Cunegündes
Data de Publicação: 2009
Outros Autores: Amaral, Livia Gomes, Borges, Franz Viana, Vieira, Hugo Paes Leme, Matta, Claudia Gomes Fernandes, Matta, Marcos Fernando de Resende
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/22698
Resumo: Keywords: IgY antibody – Tetanus toxoid – Antibody stability – ELISA – Clostridium tetani.
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spelling Guimarães, Marco Cesar CunegündesAmaral, Livia GomesBorges, Franz VianaVieira, Hugo Paes LemeMatta, Claudia Gomes FernandesMatta, Marcos Fernando de ResendeGuimarães, Marco Cesar CunegündesAmaral, Livia GomesBorges, Franz VianaVieira, Hugo Paes LemeMatta, Claudia Gomes FernandesMatta, Marcos Fernando de Resende2017-06-01T19:14:32Z2017-06-01T19:14:32Z2009-09R. Ci. méd. biol., Salvador, v.8, n.3, p.307-314, set./dez. 20091677-5090http://repositorio.ufba.br/ri/handle/ri/22698v.8, n. 3Keywords: IgY antibody – Tetanus toxoid – Antibody stability – ELISA – Clostridium tetani.Abstract: Tetanus is an acute, occasionally a fatal, disease of the central nervous system, caused by the toxin of the tetanus bacterium Clostridium tetani. The current techniques used in the diagnosis and therapeutics of tetanus diseases employ monoclonal antibodies produced in mouse. The use of monoclonal antibodies has several advantages such as homogeneity and specificity. In contrast, a remarkable characteristic of polyclonal antibodies, especially the immunoglobulin class IgY, which is extracted from poultry, is obtained in greater amount than IgG antibodies. Therefore, the alternative production of IgY polyclonal antibodies against tetanus toxoid in chicken can become of great interest for immunology industry in therapeutic and diagnosis. In this work, IgY polyclonal antibodies against tetanus toxoid were produced through immunization of chickens with isolated toxoid and measured by enzyme-linked immunosorbent assay (ELISA). The effect of storage temperature on the stability of chicken IgY was measured through indirect ELISA. Results showed that the IgY antibodies were produced with success and easily isolated. The time and temperature influence was measured through indirect ELISA. Altogether, the results indicate the use IgY polyclonal antibodies purified from chicken as an alternative for the detection of the tetanus toxin with potential applications in diagnosis and treatment of infectious diseases.Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2017-06-01T19:14:32Z No. of bitstreams: 1 10_v.8_3.pdf: 390371 bytes, checksum: fec8c68a5bd6eddfbad5af57220fddfd (MD5)Made available in DSpace on 2017-06-01T19:14:32Z (GMT). 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dc.title.pt_BR.fl_str_mv Considerations on the stability of IgY antibodies antitetanus toxoid
dc.title.alternative.pt_BR.fl_str_mv Revista de Ciências Médicas e Biológicas
title Considerations on the stability of IgY antibodies antitetanus toxoid
spellingShingle Considerations on the stability of IgY antibodies antitetanus toxoid
Guimarães, Marco Cesar Cunegündes
Anticorpos IgY
Toxina tetânica- Estabilidade
ELISA indireto
Clostridium tetani.
title_short Considerations on the stability of IgY antibodies antitetanus toxoid
title_full Considerations on the stability of IgY antibodies antitetanus toxoid
title_fullStr Considerations on the stability of IgY antibodies antitetanus toxoid
title_full_unstemmed Considerations on the stability of IgY antibodies antitetanus toxoid
title_sort Considerations on the stability of IgY antibodies antitetanus toxoid
author Guimarães, Marco Cesar Cunegündes
author_facet Guimarães, Marco Cesar Cunegündes
Amaral, Livia Gomes
Borges, Franz Viana
Vieira, Hugo Paes Leme
Matta, Claudia Gomes Fernandes
Matta, Marcos Fernando de Resende
author_role author
author2 Amaral, Livia Gomes
Borges, Franz Viana
Vieira, Hugo Paes Leme
Matta, Claudia Gomes Fernandes
Matta, Marcos Fernando de Resende
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Marco Cesar Cunegündes
Amaral, Livia Gomes
Borges, Franz Viana
Vieira, Hugo Paes Leme
Matta, Claudia Gomes Fernandes
Matta, Marcos Fernando de Resende
Guimarães, Marco Cesar Cunegündes
Amaral, Livia Gomes
Borges, Franz Viana
Vieira, Hugo Paes Leme
Matta, Claudia Gomes Fernandes
Matta, Marcos Fernando de Resende
dc.subject.por.fl_str_mv Anticorpos IgY
Toxina tetânica- Estabilidade
ELISA indireto
Clostridium tetani.
topic Anticorpos IgY
Toxina tetânica- Estabilidade
ELISA indireto
Clostridium tetani.
description Keywords: IgY antibody – Tetanus toxoid – Antibody stability – ELISA – Clostridium tetani.
publishDate 2009
dc.date.issued.fl_str_mv 2009-09
dc.date.accessioned.fl_str_mv 2017-06-01T19:14:32Z
dc.date.available.fl_str_mv 2017-06-01T19:14:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv R. Ci. méd. biol., Salvador, v.8, n.3, p.307-314, set./dez. 2009
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/22698
dc.identifier.issn.none.fl_str_mv 1677-5090
dc.identifier.number.pt_BR.fl_str_mv v.8, n. 3
identifier_str_mv R. Ci. méd. biol., Salvador, v.8, n.3, p.307-314, set./dez. 2009
1677-5090
v.8, n. 3
url http://repositorio.ufba.br/ri/handle/ri/22698
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
dc.source.pt_BR.fl_str_mv http://www.portalseer.ufba.br/index.php/cmbio/article/view/4474/3330
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