Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 2005
Outros Autores: Moura, Tatiana Rodrigues de, Novais, Fernanda O., Oliveira, Fabiano, Clarêncio, Jorge, Noronha, Almério Libório Lopes de, Barral, Aldina Maria Prado, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13599
Resumo: Texto completo: acesso restrito.p. 5827-5834
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spelling Brodskyn, Claudia IdaMoura, Tatiana Rodrigues deNovais, Fernanda O.Oliveira, FabianoClarêncio, JorgeNoronha, Almério Libório Lopes deBarral, Aldina Maria PradoOliveira, Camila Indiani deBrodskyn, Claudia IdaMoura, Tatiana Rodrigues deNovais, Fernanda O.Oliveira, FabianoClarêncio, JorgeNoronha, Almério Libório Lopes deBarral, Aldina Maria PradoOliveira, Camila Indiani de2013-11-08T18:30:58Z20050019-9567http://repositorio.ufba.br/ri/handle/ri/13599v.73 n. 9Texto completo: acesso restrito.p. 5827-5834Leishmania spp. cause a broad spectrum of diseases collectively known as leishmaniasis. Leishmania braziliensis is the main etiological agent of American cutaneous leishmaniasis (ACL) and mucocutaneous leishmaniasis. In the present study, we have developed an experimental model of infection that closely resembles ACL caused by L. braziliensis. In order to do so, BALB/c mice were infected in the ear dermis with 105 parasites and distinct aspects of the infection were evaluated. Following inoculation, parasite expansion in the ear dermis was accompanied by the development of an ulcerated dermal lesion which healed spontaneously, as seen by the presence of a scar. Histological analysis of infected ears showed the presence of a mixed inflammatory infiltrate consisting of both mononuclear and polymorphonuclear cells. In draining lymph nodes, parasite replication was detected throughout the infection. In vitro restimulation of draining lymph node cells followed by intracellular staining showed an up-regulation in the production of gamma interferon (IFN-γ) and in the frequency of IFN-γ-secreting CD4+ and CD8+ T cells. Reverse transcription-PCR of ears and draining lymph node cells showed the expression of CC chemokines. The dermal model of infection with L. braziliensis herein is able to reproduce aspects of the natural infection, such as the presence of an ulcerated lesion, parasite dissemination to lymphoid areas, and the development of a Th1-type immune response. These results indicate that this model shall be useful to address questions related to the concomitant immunity to reinfection and parasite persistence leading to mucocutaneous leishmaniasis.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-11-07T12:28:33Z No. of bitstreams: 1 Claudia Brodskyn.pdf: 408839 bytes, checksum: 78607c37704bcc5d8c99e5d1da1bbe65 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-08T18:30:58Z (GMT) No. of bitstreams: 1 Claudia Brodskyn.pdf: 408839 bytes, checksum: 78607c37704bcc5d8c99e5d1da1bbe65 (MD5)Made available in DSpace on 2013-11-08T18:30:58Z (GMT). No. of bitstreams: 1 Claudia Brodskyn.pdf: 408839 bytes, checksum: 78607c37704bcc5d8c99e5d1da1bbe65 (MD5) Previous issue date: 2005http://dx.doi.org/ 10.1128/IAI.73.9.5827-5834.2005reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAToward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensisInfection and Immunityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALClaudia Brodskyn.pdfClaudia Brodskyn.pdfapplication/pdf408839https://repositorio.ufba.br/bitstream/ri/13599/1/Claudia%20Brodskyn.pdf78607c37704bcc5d8c99e5d1da1bbe65MD51LICENSElicense.txtlicense.txttext/plain1383https://repositorio.ufba.br/bitstream/ri/13599/2/license.txt05eca2f01d0b3307819d0369dab18a34MD52TEXTClaudia Brodskyn.pdf.txtClaudia Brodskyn.pdf.txtExtracted texttext/plain45466https://repositorio.ufba.br/bitstream/ri/13599/3/Claudia%20Brodskyn.pdf.txt3f617724f3ef13c70b92689b311550f8MD53ri/135992022-08-08 13:17:40.216oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:40Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
dc.title.alternative.pt_BR.fl_str_mv Infection and Immunity
title Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
spellingShingle Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
Brodskyn, Claudia Ida
title_short Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_full Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_fullStr Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_full_unstemmed Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
title_sort Toward a Novel Experimental Model of Infection To Study American Cutaneous Leishmaniasis Caused by Leishmania braziliensis
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
Moura, Tatiana Rodrigues de
Novais, Fernanda O.
Oliveira, Fabiano
Clarêncio, Jorge
Noronha, Almério Libório Lopes de
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author_role author
author2 Moura, Tatiana Rodrigues de
Novais, Fernanda O.
Oliveira, Fabiano
Clarêncio, Jorge
Noronha, Almério Libório Lopes de
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
Moura, Tatiana Rodrigues de
Novais, Fernanda O.
Oliveira, Fabiano
Clarêncio, Jorge
Noronha, Almério Libório Lopes de
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Brodskyn, Claudia Ida
Moura, Tatiana Rodrigues de
Novais, Fernanda O.
Oliveira, Fabiano
Clarêncio, Jorge
Noronha, Almério Libório Lopes de
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
description Texto completo: acesso restrito.p. 5827-5834
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2013-11-08T18:30:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13599
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.number.pt_BR.fl_str_mv v.73 n. 9
identifier_str_mv 0019-9567
v.73 n. 9
url http://repositorio.ufba.br/ri/handle/ri/13599
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1128/IAI.73.9.5827-5834.2005
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