Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Carvalho, Augusto M.
Data de Publicação: 2013
Outros Autores: Magalhães, Andréa, Carvalho, Lucas Pedreira de, Bacellar, Maria Olívia Amado Ramos, Scott, Phillip, Carvalho Filho, Edgar Marcelino de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15925
Resumo: p. 1-8
id UFBA-2_e5ce46415c9af4d6579923ebe229dca4
oai_identifier_str oai:repositorio.ufba.br:ri/15925
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Carvalho, Augusto M.Magalhães, AndréaCarvalho, Lucas Pedreira deBacellar, Maria Olívia Amado RamosScott, PhillipCarvalho Filho, Edgar Marcelino de2014-09-09T15:19:34Z2014-09-09T15:19:34Z20131471-2334http://repositorio.ufba.br/ri/handle/ri/15925v. 13, n. 1p. 1-8Background: The main clinical forms of tegumentary leishmaniasis are cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). L.braziliensis infection is characterized by an exaggerated production of IFN-gamma and TNF-alpha, cytokines involved in parasite destruction, but also in the pathology. Maintenance of an antigen-specific immune response may be important for resistance to re-infection and will contribute for vaccine development. In the present work we investigated the immune response in CL and ML cured individuals. Methods: Participants in the present study included 20 CL and 20 ML patients, who were evaluated prior to, as well as 2 to 15 years after therapy. IFN-gamma, IL-2 and TNF-alpha production were determined by ELISA in supernatants of mononuclear cells stimulated with soluble L.braziliensis antigen (SLA). The frequency of memory CD4+ T cell populations was determined by FACS. Results: Here we show that the majority of CL and ML patients did not produce in vitro IFN-gamma in response to SLA after cure. In the cured individuals who responded to SLA, effector memory (CD45RA-CCR7-) CD4+ T cells were the ones producing IFN-gamma. Because a large percent of CL and ML cured patients lost SLA-induced IFN-gamma production in peripheral blood, we performed Leishmania skin test (LST). A positive LST was found in 87.5% and 100% of CL and ML cured individuals, respectively, who did not produce IFN-gamma or IL-2 in vitro. Conclusion: This study shows that in spite of losing in vitro antigen-specific response to Leishmania, cured CL and ML subjects retain the ability to respond to SLA in vivo. These findings indicate that LST, rather than IFN-gamma production, may be a better assessment of lasting immunity to leishmaniasis in human studies, and thus a better tool for assessing immunization after vaccine. Furthermore, in cured individuals which maintains Leishmania-specific IFN-gamma production, effector memory CD4+ T cells were the main source of this cytokine.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-07T14:31:03Z No. of bitstreams: 1 1471-2334-13-529.pdf: 588230 bytes, checksum: 97d4a8ab8afd2f9b072d25708780c334 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-09-09T15:19:34Z (GMT) No. of bitstreams: 1 1471-2334-13-529.pdf: 588230 bytes, checksum: 97d4a8ab8afd2f9b072d25708780c334 (MD5)Made available in DSpace on 2014-09-09T15:19:34Z (GMT). No. of bitstreams: 1 1471-2334-13-529.pdf: 588230 bytes, checksum: 97d4a8ab8afd2f9b072d25708780c334 (MD5) Previous issue date: 2013http://dx.doi.org/10.1186/1471-2334-13-529reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACured leishmaniasisIFN-gammaEffector memory CD4+ T cellsImmunologic response and memory T cells in subjects cured of tegumentary leishmaniasisBMC Infectious Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengLICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15925/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52ORIGINAL1471-2334-13-529.pdf1471-2334-13-529.pdfapplication/pdf588230https://repositorio.ufba.br/bitstream/ri/15925/1/1471-2334-13-529.pdf97d4a8ab8afd2f9b072d25708780c334MD51TEXT1471-2334-13-529.pdf.txt1471-2334-13-529.pdf.txtExtracted texttext/plain35079https://repositorio.ufba.br/bitstream/ri/15925/3/1471-2334-13-529.pdf.txt58e3ccba6eca70623bc87dd8edf492eeMD53ri/159252022-02-21 00:10:30.541oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-02-21T03:10:30Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
dc.title.alternative.pt_BR.fl_str_mv BMC Infectious Diseases
title Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
spellingShingle Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
Carvalho, Augusto M.
Cured leishmaniasis
IFN-gamma
Effector memory CD4+ T cells
title_short Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
title_full Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
title_fullStr Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
title_full_unstemmed Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
title_sort Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis
author Carvalho, Augusto M.
author_facet Carvalho, Augusto M.
Magalhães, Andréa
Carvalho, Lucas Pedreira de
Bacellar, Maria Olívia Amado Ramos
Scott, Phillip
Carvalho Filho, Edgar Marcelino de
author_role author
author2 Magalhães, Andréa
Carvalho, Lucas Pedreira de
Bacellar, Maria Olívia Amado Ramos
Scott, Phillip
Carvalho Filho, Edgar Marcelino de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Augusto M.
Magalhães, Andréa
Carvalho, Lucas Pedreira de
Bacellar, Maria Olívia Amado Ramos
Scott, Phillip
Carvalho Filho, Edgar Marcelino de
dc.subject.por.fl_str_mv Cured leishmaniasis
IFN-gamma
Effector memory CD4+ T cells
topic Cured leishmaniasis
IFN-gamma
Effector memory CD4+ T cells
description p. 1-8
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-09-09T15:19:34Z
dc.date.available.fl_str_mv 2014-09-09T15:19:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15925
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.number.pt_BR.fl_str_mv v. 13, n. 1
identifier_str_mv 1471-2334
v. 13, n. 1
url http://repositorio.ufba.br/ri/handle/ri/15925
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1186/1471-2334-13-529
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/15925/2/license.txt
https://repositorio.ufba.br/bitstream/ri/15925/1/1471-2334-13-529.pdf
https://repositorio.ufba.br/bitstream/ri/15925/3/1471-2334-13-529.pdf.txt
bitstream.checksum.fl_str_mv 0d4b811ef71182510d2015daa7c8a900
97d4a8ab8afd2f9b072d25708780c334
58e3ccba6eca70623bc87dd8edf492ee
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459475180322816

Registros relacionados