Hepatocellular ballooning in NASH Stephen

Detalhes bibliográficos
Autor(a) principal: Caldwell, Stephen H.
Data de Publicação: 2010
Outros Autores: Ikura, Yoshihiro, Dias, Daniela, Isomoto, Kosuke
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5601
Resumo: Acesso restrito: Texto completo. p. 719-723.
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spelling Caldwell, Stephen H.Ikura, YoshihiroDias, DanielaIsomoto, KosukeCaldwell, Stephen H.Ikura, YoshihiroDias, DanielaIsomoto, Kosuke2012-04-12T13:39:38Z20100168-8278http://www.repositorio.ufba.br/ri/handle/ri/5601v. 53, n. 4Acesso restrito: Texto completo. p. 719-723.Background & Aims: Hepatocellular ballooning is a key finding in nonalcoholic steatohepatitis (NASH). It is conventionally defined by hemotoxylin and eosin (H&E) staining showing enlarged cells with rarefied cytoplasm and recently by changes in the cytoskeleton. Fat droplets are emerging as important organelles in cell metabolism. To address a possible relation between fat droplets and ballooning, we studied fat staining, H&E, and keratin 18 staining in human NASH. Methods: Sequential staining and high resolution imaging were used to study freshly prepared cryo-sections from 10 patients with histologically confirmed steatohepatitis using oil red O for fat droplet identification, H&E to identify ballooning, and anti-K18 to confirm cytoskeletal changes. High resolution images were captured at each stage using the Aperio Scanscope. To provide ultrastructural correlation, glutaraldehyde-fixed specimens were studied using transmission electron microscopy (TEM) with serial sectioning for localization of ballooned cells by light microscopy and TEM in identical specimens. Results: Serial staining consistently demonstrated that hepatocellular ballooning is associated with fat droplet accumulation evident by oil red O positivity and depletion of cytoplasmic keratin 18 with K-18 positive Mallory–Denk bodies (MDB). TEM confirmed the association between osmium stained fat droplets,MDB formation, and cellular enlargement and suggested droplet-associated dilation of the endoplasmic reticulum. Conclusions: These results indicate a relationship between cellular ballooning, fat droplet accumulation, and cytoskeletal injury in NASH. We speculate that injury to multiple organelles, including fat droplets and endoplasmic reticulum, contribute to this characteristic finding.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-04-12T13:39:38Z No. of bitstreams: 1 __ac.els-cdn.com_S016882...471d89bd5e9f6859c6ae2456a7588.pdf: 442020 bytes, checksum: 9bf5f491c52b1d7cb3d56d113b992b12 (MD5)Made available in DSpace on 2012-04-12T13:39:38Z (GMT). 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dc.title.pt_BR.fl_str_mv Hepatocellular ballooning in NASH Stephen
dc.title.alternative.pt_BR.fl_str_mv JOURNAL OF HEPATOLOGY
title Hepatocellular ballooning in NASH Stephen
spellingShingle Hepatocellular ballooning in NASH Stephen
Caldwell, Stephen H.
Cellular ballooning
Steatohepatitis
NASH
NAFLD
Keratin 18
Fat staining
Oil red O
title_short Hepatocellular ballooning in NASH Stephen
title_full Hepatocellular ballooning in NASH Stephen
title_fullStr Hepatocellular ballooning in NASH Stephen
title_full_unstemmed Hepatocellular ballooning in NASH Stephen
title_sort Hepatocellular ballooning in NASH Stephen
author Caldwell, Stephen H.
author_facet Caldwell, Stephen H.
Ikura, Yoshihiro
Dias, Daniela
Isomoto, Kosuke
author_role author
author2 Ikura, Yoshihiro
Dias, Daniela
Isomoto, Kosuke
author2_role author
author
author
dc.contributor.author.fl_str_mv Caldwell, Stephen H.
Ikura, Yoshihiro
Dias, Daniela
Isomoto, Kosuke
Caldwell, Stephen H.
Ikura, Yoshihiro
Dias, Daniela
Isomoto, Kosuke
dc.subject.por.fl_str_mv Cellular ballooning
Steatohepatitis
NASH
NAFLD
Keratin 18
Fat staining
Oil red O
topic Cellular ballooning
Steatohepatitis
NASH
NAFLD
Keratin 18
Fat staining
Oil red O
description Acesso restrito: Texto completo. p. 719-723.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-04-12T13:39:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5601
dc.identifier.issn.none.fl_str_mv 0168-8278
dc.identifier.number.pt_BR.fl_str_mv v. 53, n. 4
identifier_str_mv 0168-8278
v. 53, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/5601
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv Journal of Hepatology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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