Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4784 |
Resumo: | p. 485 - 492 |
id |
UFBA-2_e66b9e33882b2d12b2c510dab035361c |
---|---|
oai_identifier_str |
oai:repositorio.ufba.br:ri/4784 |
network_acronym_str |
UFBA-2 |
network_name_str |
Repositório Institucional da UFBA |
repository_id_str |
1932 |
spelling |
Pinheiro, Antonio Luiz BarbosaPozza, Daniel HumbertoOliveira, Marília Gerhardt deWeismann, RubenRamalho, Luciana Maria PedreiraPinheiro, Antonio Luiz BarbosaPozza, Daniel HumbertoOliveira, Marília Gerhardt deWeismann, RubenRamalho, Luciana Maria Pedreira2011-12-06T13:57:19Z2011-12-06T13:57:19Z20051044-5471http://www.repositorio.ufba.br/ri/handle/ri/4784v. 23, n. 5.p. 485 - 492OBJECTIVE: This study aimed to describe, through morphologic and cytochemical analysis, the healing process of wounds submitted (or not) to laser therapy (lambda685 nm) or polarized light (lambda400-2000 nm). BACKGROUND DATA: There are many reports on different effects of several types of phototherapies on the treatment of distinct conditions, amongst them, on wound healing. Laser therapy and the use of polarized light are still controversial despite successive reports on their positive effects on several biological processes. METHODS: Thirty male Wistar rats, approximately 4 months old, were used, and standardized excisional wounds were created on their dorsum. The wounds were irradiated in four equidistant points with laser light or illuminated with polarized light, both with doses of 20 or 40 J/cm2. Group 1 acted as untreated controls. Animals were irradiated every 48 h during 7 days, starting immediately after surgery, and were humanely killed on the 8th post-operative day. Specimens were taken and routinely processed and stained with H&E, and for descriptive analysis of myofibroblasts and collagen fibers, the specimens were imunnomarked by smooth muscle alpha-actin and picrosirius stain. RESULTS: Control specimens showed the presence of ulceration, hyperemia, discrete edema, intense, and diffuse inflammation, collagen deposition was irregular, and myofibroblasts were seen parallel to the wound margins. Wounds treated by laser therapy with a dose of 20 J/cm2 showed mild hyperemia, inflammation varied from moderate to intense, the number of fibroblasts was large, and the distribution of collagen fibers was more regular. Increasing the dose to 40 J/cm2 evidenced exuberant neovascularization, severe hyperemia, moderate to severe inflammation, large collagen deposition, and fewer myofibroblasts. On subjects illuminated with polarized light with a dose of 20 J/cm2, mild to moderate hyperemia was detectable, and collagen matrix was expressive and unevenly distributed; a larger number of myofibroblasts was present and no re-epithelialization was seen. Increasing the dose resulted in mild to moderate hyperemia, no re-epithelialization was seen, edema was discrete, and inflammation was moderate. CONCLUSION: The use of 685-nm laser light or polarized light with a dose of 20 J/cm2 resulted in increased collagen deposition and better organization on healing wounds, and the number of myofibroblast was increased when polarized light is used.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2011-12-06T13:57:19Z No. of bitstreams: 1 Polarized_Light.pdf: 1649216 bytes, checksum: bcb262cb882efa338ad0141af0e96fb0 (MD5)Made available in DSpace on 2011-12-06T13:57:19Z (GMT). No. of bitstreams: 1 Polarized_Light.pdf: 1649216 bytes, checksum: bcb262cb882efa338ad0141af0e96fb0 (MD5) Previous issue date: 2005Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysisJournal of Clinical Laser Medicine & Surgeryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALPolarized_Light.pdfPolarized_Light.pdfapplication/pdf1649216https://repositorio.ufba.br/bitstream/ri/4784/1/Polarized_Light.pdfbcb262cb882efa338ad0141af0e96fb0MD51LICENSElicense.txtlicense.txttext/plain1748https://repositorio.ufba.br/bitstream/ri/4784/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTPolarized_Light.pdf.txtPolarized_Light.pdf.txtExtracted texttext/plain34324https://repositorio.ufba.br/bitstream/ri/4784/3/Polarized_Light.pdf.txt8bc81ce809af797520f09bfcf2e14adeMD53ri/47842022-07-05 14:03:17.604oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:17Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
dc.title.alternative.pt_BR.fl_str_mv |
Journal of Clinical Laser Medicine & Surgery |
title |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
spellingShingle |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis Pinheiro, Antonio Luiz Barbosa |
title_short |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
title_full |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
title_fullStr |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
title_full_unstemmed |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
title_sort |
Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis |
author |
Pinheiro, Antonio Luiz Barbosa |
author_facet |
Pinheiro, Antonio Luiz Barbosa Pozza, Daniel Humberto Oliveira, Marília Gerhardt de Weismann, Ruben Ramalho, Luciana Maria Pedreira |
author_role |
author |
author2 |
Pozza, Daniel Humberto Oliveira, Marília Gerhardt de Weismann, Ruben Ramalho, Luciana Maria Pedreira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pinheiro, Antonio Luiz Barbosa Pozza, Daniel Humberto Oliveira, Marília Gerhardt de Weismann, Ruben Ramalho, Luciana Maria Pedreira Pinheiro, Antonio Luiz Barbosa Pozza, Daniel Humberto Oliveira, Marília Gerhardt de Weismann, Ruben Ramalho, Luciana Maria Pedreira |
description |
p. 485 - 492 |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005 |
dc.date.accessioned.fl_str_mv |
2011-12-06T13:57:19Z |
dc.date.available.fl_str_mv |
2011-12-06T13:57:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/4784 |
dc.identifier.issn.none.fl_str_mv |
1044-5471 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 23, n. 5. |
identifier_str_mv |
1044-5471 v. 23, n. 5. |
url |
http://www.repositorio.ufba.br/ri/handle/ri/4784 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Repositório Institucional da UFBA |
collection |
Repositório Institucional da UFBA |
bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/4784/1/Polarized_Light.pdf https://repositorio.ufba.br/bitstream/ri/4784/2/license.txt https://repositorio.ufba.br/bitstream/ri/4784/3/Polarized_Light.pdf.txt |
bitstream.checksum.fl_str_mv |
bcb262cb882efa338ad0141af0e96fb0 8a4605be74aa9ea9d79846c1fba20a33 8bc81ce809af797520f09bfcf2e14ade |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
|
_version_ |
1808459389659512832 |