Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Antonio Luiz Barbosa
Data de Publicação: 2005
Outros Autores: Pozza, Daniel Humberto, Oliveira, Marília Gerhardt de, Weismann, Ruben, Ramalho, Luciana Maria Pedreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/4784
Resumo: p. 485 - 492
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spelling Pinheiro, Antonio Luiz BarbosaPozza, Daniel HumbertoOliveira, Marília Gerhardt deWeismann, RubenRamalho, Luciana Maria PedreiraPinheiro, Antonio Luiz BarbosaPozza, Daniel HumbertoOliveira, Marília Gerhardt deWeismann, RubenRamalho, Luciana Maria Pedreira2011-12-06T13:57:19Z2011-12-06T13:57:19Z20051044-5471http://www.repositorio.ufba.br/ri/handle/ri/4784v. 23, n. 5.p. 485 - 492OBJECTIVE: This study aimed to describe, through morphologic and cytochemical analysis, the healing process of wounds submitted (or not) to laser therapy (lambda685 nm) or polarized light (lambda400-2000 nm). BACKGROUND DATA: There are many reports on different effects of several types of phototherapies on the treatment of distinct conditions, amongst them, on wound healing. Laser therapy and the use of polarized light are still controversial despite successive reports on their positive effects on several biological processes. METHODS: Thirty male Wistar rats, approximately 4 months old, were used, and standardized excisional wounds were created on their dorsum. The wounds were irradiated in four equidistant points with laser light or illuminated with polarized light, both with doses of 20 or 40 J/cm2. Group 1 acted as untreated controls. Animals were irradiated every 48 h during 7 days, starting immediately after surgery, and were humanely killed on the 8th post-operative day. Specimens were taken and routinely processed and stained with H&E, and for descriptive analysis of myofibroblasts and collagen fibers, the specimens were imunnomarked by smooth muscle alpha-actin and picrosirius stain. RESULTS: Control specimens showed the presence of ulceration, hyperemia, discrete edema, intense, and diffuse inflammation, collagen deposition was irregular, and myofibroblasts were seen parallel to the wound margins. Wounds treated by laser therapy with a dose of 20 J/cm2 showed mild hyperemia, inflammation varied from moderate to intense, the number of fibroblasts was large, and the distribution of collagen fibers was more regular. Increasing the dose to 40 J/cm2 evidenced exuberant neovascularization, severe hyperemia, moderate to severe inflammation, large collagen deposition, and fewer myofibroblasts. On subjects illuminated with polarized light with a dose of 20 J/cm2, mild to moderate hyperemia was detectable, and collagen matrix was expressive and unevenly distributed; a larger number of myofibroblasts was present and no re-epithelialization was seen. Increasing the dose resulted in mild to moderate hyperemia, no re-epithelialization was seen, edema was discrete, and inflammation was moderate. CONCLUSION: The use of 685-nm laser light or polarized light with a dose of 20 J/cm2 resulted in increased collagen deposition and better organization on healing wounds, and the number of myofibroblast was increased when polarized light is used.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2011-12-06T13:57:19Z No. of bitstreams: 1 Polarized_Light.pdf: 1649216 bytes, checksum: bcb262cb882efa338ad0141af0e96fb0 (MD5)Made available in DSpace on 2011-12-06T13:57:19Z (GMT). No. of bitstreams: 1 Polarized_Light.pdf: 1649216 bytes, checksum: bcb262cb882efa338ad0141af0e96fb0 (MD5) Previous issue date: 2005Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysisJournal of Clinical Laser Medicine & Surgeryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALPolarized_Light.pdfPolarized_Light.pdfapplication/pdf1649216https://repositorio.ufba.br/bitstream/ri/4784/1/Polarized_Light.pdfbcb262cb882efa338ad0141af0e96fb0MD51LICENSElicense.txtlicense.txttext/plain1748https://repositorio.ufba.br/bitstream/ri/4784/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTPolarized_Light.pdf.txtPolarized_Light.pdf.txtExtracted texttext/plain34324https://repositorio.ufba.br/bitstream/ri/4784/3/Polarized_Light.pdf.txt8bc81ce809af797520f09bfcf2e14adeMD53ri/47842022-07-05 14:03:17.604oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:17Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
dc.title.alternative.pt_BR.fl_str_mv Journal of Clinical Laser Medicine & Surgery
title Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
spellingShingle Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
Pinheiro, Antonio Luiz Barbosa
title_short Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
title_full Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
title_fullStr Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
title_full_unstemmed Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
title_sort Polarized light (400-2000nm) and non-ablative Laser (685nm): a description of the wound healing process using immunohistochemical analysis
author Pinheiro, Antonio Luiz Barbosa
author_facet Pinheiro, Antonio Luiz Barbosa
Pozza, Daniel Humberto
Oliveira, Marília Gerhardt de
Weismann, Ruben
Ramalho, Luciana Maria Pedreira
author_role author
author2 Pozza, Daniel Humberto
Oliveira, Marília Gerhardt de
Weismann, Ruben
Ramalho, Luciana Maria Pedreira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, Antonio Luiz Barbosa
Pozza, Daniel Humberto
Oliveira, Marília Gerhardt de
Weismann, Ruben
Ramalho, Luciana Maria Pedreira
Pinheiro, Antonio Luiz Barbosa
Pozza, Daniel Humberto
Oliveira, Marília Gerhardt de
Weismann, Ruben
Ramalho, Luciana Maria Pedreira
description p. 485 - 492
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2011-12-06T13:57:19Z
dc.date.available.fl_str_mv 2011-12-06T13:57:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/4784
dc.identifier.issn.none.fl_str_mv 1044-5471
dc.identifier.number.pt_BR.fl_str_mv v. 23, n. 5.
identifier_str_mv 1044-5471
v. 23, n. 5.
url http://www.repositorio.ufba.br/ri/handle/ri/4784
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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collection Repositório Institucional da UFBA
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