A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12

Detalhes bibliográficos
Autor(a) principal: Skeiky, Yasir A. W.
Data de Publicação: 1995
Outros Autores: Guderian, Jeffrey A., Benson, Darin R., Bacelar, Olivia, Carvalho Filho, Edgar Marcelino de, Kubin, Marek, Badaró, Roberto José da Silva, Trinchieri, Giorgio, Reed, Steven G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13538
Resumo: p. 1527-1537
id UFBA-2_e9ebca620e7d398c06e7c44eea0850f0
oai_identifier_str oai:repositorio.ufba.br:ri/13538
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Skeiky, Yasir A. W.Guderian, Jeffrey A.Benson, Darin R.Bacelar, OliviaCarvalho Filho, Edgar Marcelino deKubin, MarekBadaró, Roberto José da SilvaTrinchieri, GiorgioReed, Steven G.Skeiky, Yasir A. W.Guderian, Jeffrey A.Benson, Darin R.Bacelar, OliviaCarvalho Filho, Edgar Marcelino deKubin, MarekBadaró, Roberto José da SilvaTrinchieri, GiorgioReed, Steven G.2013-11-03T15:02:35Z2013-11-03T15:02:35Z19950022-1007http://repositorio.ufba.br/ri/handle/ri/13538v. 181p. 1527-1537Leishmania braziliensis causes cutaneous and mucosal leishmaniasis in humans. Most patients with cutaneous leishmaniasis heal spontaneously and may therefore have developed protective immunity. There appears to be a mixed cytokine profile associated with active cutaneous or mucosal disease, and a dominant T helper (Th)l-type response associated with healing. Leishmanial antigens that elicit these potent proliferative and cytokine responses from peripheral blood mononuclear cells (PBMC) are now being identified. Herein, we report on the cloning and expression of a L. braziliensis gene homologous to the eukaryotic ribosomal protein elF4A (LelF) and patient PBMC responses to rLelF. Patients with mucosal and self-healing cutaneous disease had significantly higher proliferative responses than those with cutaneous lesions. Whereas the parasite lysate stimulated patient PBMC to produce a mixed Thl/Th2-type cytokine profile, LelF stimulated the production of interferon ~/(IFN-3'), interleukin 2 (IL-2), and tumor necrosis factor ot but not IL-4 or IL-10. Recombinant 1.elF (rLelF) downregulated both IL-10 mlLNA in the "resting" PBMC of leishmaniasis patients and LPS-induced IL-10 production by patient PBMC. rLelF also stimulated the production of IL-12 in cultured PBMC from both patients and uninfected individuals. The production of IFN-3' by patient PBMC stimulated with either rLelF or parasite lysate was IL-12 dependent, whereas anti-IFN-3, monoclonal antibody only partially blocked the LelF-induced production of IL-12. In vitro production of both IFN-'y and IL-12 was abrogated by exogenous human recombinant IL-10. Therefore, we have identified a recombinant leishmanial antigen that elicits IL-12 production and Thl-type responses in patients as well as IL-12 production in normal human PBMC.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-09-16T17:27:13Z No. of bitstreams: 1 11111aaw.pdf: 2505648 bytes, checksum: 5976238837c366626c39bab0545a89c0 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-03T15:02:35Z (GMT) No. of bitstreams: 1 11111aaw.pdf: 2505648 bytes, checksum: 5976238837c366626c39bab0545a89c0 (MD5)Made available in DSpace on 2013-11-03T15:02:35Z (GMT). No. of bitstreams: 1 11111aaw.pdf: 2505648 bytes, checksum: 5976238837c366626c39bab0545a89c0 (MD5) Previous issue date: 1995SalvadorJournal of Experimental Medicinehttp://jem.rupress.org/content/181/4/1527.full.pdf+htmlreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAA recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12Journal of Experimental Medicineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL11111aaw.pdf11111aaw.pdfapplication/pdf2505648https://repositorio.ufba.br/bitstream/ri/13538/1/11111aaw.pdf5976238837c366626c39bab0545a89c0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13538/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT11111aaw.pdf.txt11111aaw.pdf.txtExtracted texttext/plain913https://repositorio.ufba.br/bitstream/ri/13538/3/11111aaw.pdf.txte2b46b88b4cbbfe8c83111af228b7fefMD53ri/135382022-07-05 14:03:20.704oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:20Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
dc.title.alternative.pt_BR.fl_str_mv Journal of Experimental Medicine
title A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
spellingShingle A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
Skeiky, Yasir A. W.
title_short A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
title_full A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
title_fullStr A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
title_full_unstemmed A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
title_sort A recombinant leishmania antigen that stimulates human peripheral blood mononuclear cells to express a th1-type cytokine profile and to produce interleukin 12
author Skeiky, Yasir A. W.
author_facet Skeiky, Yasir A. W.
Guderian, Jeffrey A.
Benson, Darin R.
Bacelar, Olivia
Carvalho Filho, Edgar Marcelino de
Kubin, Marek
Badaró, Roberto José da Silva
Trinchieri, Giorgio
Reed, Steven G.
author_role author
author2 Guderian, Jeffrey A.
Benson, Darin R.
Bacelar, Olivia
Carvalho Filho, Edgar Marcelino de
Kubin, Marek
Badaró, Roberto José da Silva
Trinchieri, Giorgio
Reed, Steven G.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Skeiky, Yasir A. W.
Guderian, Jeffrey A.
Benson, Darin R.
Bacelar, Olivia
Carvalho Filho, Edgar Marcelino de
Kubin, Marek
Badaró, Roberto José da Silva
Trinchieri, Giorgio
Reed, Steven G.
Skeiky, Yasir A. W.
Guderian, Jeffrey A.
Benson, Darin R.
Bacelar, Olivia
Carvalho Filho, Edgar Marcelino de
Kubin, Marek
Badaró, Roberto José da Silva
Trinchieri, Giorgio
Reed, Steven G.
description p. 1527-1537
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2013-11-03T15:02:35Z
dc.date.available.fl_str_mv 2013-11-03T15:02:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13538
dc.identifier.issn.none.fl_str_mv 0022-1007
dc.identifier.number.pt_BR.fl_str_mv v. 181
identifier_str_mv 0022-1007
v. 181
url http://repositorio.ufba.br/ri/handle/ri/13538
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Journal of Experimental Medicine
publisher.none.fl_str_mv Journal of Experimental Medicine
dc.source.pt_BR.fl_str_mv http://jem.rupress.org/content/181/4/1527.full.pdf+html
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/13538/1/11111aaw.pdf
https://repositorio.ufba.br/bitstream/ri/13538/2/license.txt
https://repositorio.ufba.br/bitstream/ri/13538/3/11111aaw.pdf.txt
bitstream.checksum.fl_str_mv 5976238837c366626c39bab0545a89c0
1b89a9a0548218172d7c829f87a0eab9
e2b46b88b4cbbfe8c83111af228b7fef
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459459003940864

Registros relacionados