Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/17866 |
Resumo: | Texto completo: acesso restrito. p. 575-582 |
| id |
UFBA-2_f2f87ac1272a21a4c0defe050bdb7757 |
|---|---|
| oai_identifier_str |
oai:repositorio.ufba.br:ri/17866 |
| network_acronym_str |
UFBA-2 |
| network_name_str |
Repositório Institucional da UFBA |
| repository_id_str |
1932 |
| spelling |
Santos, Théo AraújoLuz, Nívea FariasAndrade, Bruno BezerrilCosta, Jaqueline FrançaAfonso, LilianClarêncio, JorgeMiranda, José CarlosBozza, Patrícia TorresReis, George A. dosBrodskyn, Claudia IdaBarral-Netto, ManoelBorges, Valéria de MatosBarral, Aldina Maria Prado2015-06-10T18:42:43Z20110741-5400http://repositorio.ufba.br/ri/handle/ri/17866v. 90, n. 3Texto completo: acesso restrito. p. 575-582Neutrophils are considered the host's first line of defense against infections and have been implicated in the immunopathogenesis of Leishmaniasis. Leishmania parasites are inoculated alongside vectors' saliva, which is a rich source of pharmacologically active substances that interfere with host immune response. In the present study, we tested the hypothesis that salivary components from Lutzomyia longipalpis, an important vector of visceral Leishmaniasis, enhance neutrophil apoptosis. Murine inflammatory peritoneal neutrophils cultured in the presence of SGS presented increased surface expression of FasL and underwent caspase-dependent and FasL-mediated apoptosis. This proapoptosis effect of SGS on neutrophils was abrogated by pretreatment with protease as well as preincubation with antisaliva antibodies. Furthermore, in the presence of Leishmania chagasi, SGS also increased apoptosis on neutrophils and increased PGE2 release and decreased ROS production by neutrophils, while enhancing parasite viability inside these cells. The increased parasite burden was abrogated by treatment with z-VAD, a pan caspase inhibitor, and NS-398, a COX-2 inhibitor. In the presence of SGS, Leishmania-infected neutrophils produced higher levels of MCP-1 and attracted a high number of macrophages by chemotaxis in vitro assays. Both of these events were abrogated by pretreatment of neutrophils with bindarit, an inhibitor of CCL2/MCP-1 expression. Taken together, our data support the hypothesis that vector salivary proteins trigger caspase-dependent and FasL-mediated apoptosis, thereby favoring Leishmania survival inside neutrophils, which may represent an important mechanism for the establishment of Leishmania infection.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-15T13:32:29Z No. of bitstreams: 1 Théo Araújo Santos.pdf: 1136176 bytes, checksum: af97d4d5bb6968e2f71f1a300b84d15d (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2015-06-10T18:42:43Z (GMT) No. of bitstreams: 1 Théo Araújo Santos.pdf: 1136176 bytes, checksum: af97d4d5bb6968e2f71f1a300b84d15d (MD5)Made available in DSpace on 2015-06-10T18:42:43Z (GMT). No. of bitstreams: 1 Théo Araújo Santos.pdf: 1136176 bytes, checksum: af97d4d5bb6968e2f71f1a300b84d15d (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1189/jlb.0211105reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania chagasiSand flyCell deathFasLChemotaxisLutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophilsJournal of Leukocyte Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALThéo Araújo Santos.pdfThéo Araújo Santos.pdfapplication/pdf1136176https://repositorio.ufba.br/bitstream/ri/17866/1/Th%c3%a9o%20Ara%c3%bajo%20Santos.pdfaf97d4d5bb6968e2f71f1a300b84d15dMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/17866/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTThéo Araújo Santos.pdf.txtThéo Araújo Santos.pdf.txtExtracted texttext/plain47921https://repositorio.ufba.br/bitstream/ri/17866/3/Th%c3%a9o%20Ara%c3%bajo%20Santos.pdf.txtd607eeeb2d7f6b753bdc7a97a2923b8fMD53ri/178662022-07-01 10:46:54.412oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-01T13:46:54Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Leukocyte Biology |
| title |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| spellingShingle |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils Santos, Théo Araújo Leishmania chagasi Sand fly Cell death FasL Chemotaxis |
| title_short |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| title_full |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| title_fullStr |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| title_full_unstemmed |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| title_sort |
Lutzomyia longipalpis saliva drives apoptosis and enhances parasite burden in neutrophils |
| author |
Santos, Théo Araújo |
| author_facet |
Santos, Théo Araújo Luz, Nívea Farias Andrade, Bruno Bezerril Costa, Jaqueline França Afonso, Lilian Clarêncio, Jorge Miranda, José Carlos Bozza, Patrícia Torres Reis, George A. dos Brodskyn, Claudia Ida Barral-Netto, Manoel Borges, Valéria de Matos Barral, Aldina Maria Prado |
| author_role |
author |
| author2 |
Luz, Nívea Farias Andrade, Bruno Bezerril Costa, Jaqueline França Afonso, Lilian Clarêncio, Jorge Miranda, José Carlos Bozza, Patrícia Torres Reis, George A. dos Brodskyn, Claudia Ida Barral-Netto, Manoel Borges, Valéria de Matos Barral, Aldina Maria Prado |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Théo Araújo Luz, Nívea Farias Andrade, Bruno Bezerril Costa, Jaqueline França Afonso, Lilian Clarêncio, Jorge Miranda, José Carlos Bozza, Patrícia Torres Reis, George A. dos Brodskyn, Claudia Ida Barral-Netto, Manoel Borges, Valéria de Matos Barral, Aldina Maria Prado |
| dc.subject.por.fl_str_mv |
Leishmania chagasi Sand fly Cell death FasL Chemotaxis |
| topic |
Leishmania chagasi Sand fly Cell death FasL Chemotaxis |
| description |
Texto completo: acesso restrito. p. 575-582 |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011 |
| dc.date.accessioned.fl_str_mv |
2015-06-10T18:42:43Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/17866 |
| dc.identifier.issn.none.fl_str_mv |
0741-5400 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 90, n. 3 |
| identifier_str_mv |
0741-5400 v. 90, n. 3 |
| url |
http://repositorio.ufba.br/ri/handle/ri/17866 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/ 10.1189/jlb.0211105 |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
| instname_str |
Universidade Federal da Bahia (UFBA) |
| instacron_str |
UFBA |
| institution |
UFBA |
| reponame_str |
Repositório Institucional da UFBA |
| collection |
Repositório Institucional da UFBA |
| bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/17866/1/Th%c3%a9o%20Ara%c3%bajo%20Santos.pdf https://repositorio.ufba.br/bitstream/ri/17866/2/license.txt https://repositorio.ufba.br/bitstream/ri/17866/3/Th%c3%a9o%20Ara%c3%bajo%20Santos.pdf.txt |
| bitstream.checksum.fl_str_mv |
af97d4d5bb6968e2f71f1a300b84d15d 0d4b811ef71182510d2015daa7c8a900 d607eeeb2d7f6b753bdc7a97a2923b8f |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
| repository.mail.fl_str_mv |
|
| _version_ |
1801502532468473856 |