Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 2000
Outros Autores: Beverley, S. M., Titus, R. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/12449
Resumo: Texto completo. Acesso restrito. p. 299-304
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spelling Brodskyn, Claudia IdaBeverley, S. M.Titus, R. G.Brodskyn, Claudia IdaBeverley, S. M.Titus, R. G.2013-08-05T13:12:28Z20000009-9104http://www.repositorio.ufba.br/ri/handle/ri/12449Texto completo. Acesso restrito. p. 299-304In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-tsÿ) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-tsÿ did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the ®rst stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-30T17:10:30Z No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5)Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-08-05T13:12:28Z (GMT) No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5)Made available in DSpace on 2013-08-05T13:12:28Z (GMT). No. of bitstreams: 1 22222222222222222.pdf: 170228 bytes, checksum: 0c51c356e4df61ea77e5d3b8677bc3d5 (MD5) Previous issue date: 2000SalvadorClinical and Experimental Immunology10.1046/j.1365-2249.2000.01122.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania majorMacrophagesTh1Th2IL-12Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitroClinical and Experimental Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL22222222222222222.pdf22222222222222222.pdfapplication/pdf170228https://repositorio.ufba.br/bitstream/ri/12449/1/22222222222222222.pdf0c51c356e4df61ea77e5d3b8677bc3d5MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12449/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT22222222222222222.pdf.txt22222222222222222.pdf.txtExtracted texttext/plain30865https://repositorio.ufba.br/bitstream/ri/12449/3/22222222222222222.pdf.txt2da37ce0a02c5b70b29e9f75687833d9MD53ri/124492022-08-08 13:17:40.23oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:40Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
dc.title.alternative.pt_BR.fl_str_mv Clinical and Experimental Immunology
title Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
spellingShingle Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
Brodskyn, Claudia Ida
Leishmania major
Macrophages
Th1
Th2
IL-12
title_short Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
title_full Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
title_fullStr Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
title_full_unstemmed Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
title_sort Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
Beverley, S. M.
Titus, R. G.
author_role author
author2 Beverley, S. M.
Titus, R. G.
author2_role author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
Beverley, S. M.
Titus, R. G.
Brodskyn, Claudia Ida
Beverley, S. M.
Titus, R. G.
dc.subject.por.fl_str_mv Leishmania major
Macrophages
Th1
Th2
IL-12
topic Leishmania major
Macrophages
Th1
Th2
IL-12
description Texto completo. Acesso restrito. p. 299-304
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-08-05T13:12:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12449
dc.identifier.issn.none.fl_str_mv 0009-9104
identifier_str_mv 0009-9104
url http://www.repositorio.ufba.br/ri/handle/ri/12449
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Clinical and Experimental Immunology
publisher.none.fl_str_mv Clinical and Experimental Immunology
dc.source.pt_BR.fl_str_mv 10.1046/j.1365-2249.2000.01122.x
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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