Up-regulation of th1-type responses in mucosal leishmaniasis patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2002 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7437 |
Resumo: | Texto completo: acesso restrito. p. 6734–6740 |
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Bacellar, Maria Olívia Amado RamosLessa, Hélio AndradeSchriefer, Nicolaus Albert BorgesMachado, Paulo Roberto LimaJesus, Amélia Ribeiro deDutra, Walderez OrnelasGollob, Kenneth JohnCarvalho Filho, Edgar Marcelino deBacellar, Maria Olívia Amado RamosLessa, Hélio AndradeSchriefer, Nicolaus Albert BorgesMachado, Paulo Roberto LimaJesus, Amélia Ribeiro deDutra, Walderez OrnelasGollob, Kenneth JohnCarvalho Filho, Edgar Marcelino de2012-12-12T10:46:55Z20020019-9567http://www.repositorio.ufba.br/ri/handle/ri/7437v. 70, n. 12Texto completo: acesso restrito. p. 6734–6740The cytokine profile produced by peripheral blood mononuclear cells (PBMC) in response to leishmania antigens and the ability of interleukin-10 (IL-10) and transforming growth factor β (TGF-β) to modulate the immune response were evaluated in 21 mucosal leishmaniasis patients. Patients with mucosal disease exhibited increased gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion and decreased IL-10 secretion compared to patients with classical cutaneous leishmaniasis. CD4+ Th1 cells were the main source of IFN-γ and TNF-α production in mucosal leishmaniasis patients. Evaluation of cytokine gene expression in PBMC of these patients showed that there was strong up-regulation of IFN-γ transcripts upon stimulation with leishmania antigen, in contrast to the baseline levels of IL-10 mRNA. IL-10 suppressed IFN-γ production by 48% in cell cultures from cutaneous leishmaniasis patients and by 86% in cell cultures from healthy subjects stimulated with purified protein derivative, whereas in similar conditions IL-10 suppressed IFN-γ production by 19% in cell cultures from mucosal leishmaniasis patients stimulated with leishmania antigen. TGF-β suppressed IFN-γ levels to a greater extent in healthy subjects than in mucosal leishmaniasis and cutaneous leishmaniasis patients. These data indicate that a poorly modulated T-cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines, such as IFN-γ and TNF-α, as well as a decreased ability of IL-10 and TGF-β to modulate this response. These abnormalities may be the basis for the pathological findings observed in this disease.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-12T10:46:55Z No. of bitstreams: 1 6734.full.pdf: 263447 bytes, checksum: 38f2fe85c1a8292561d80c5c68130e2c (MD5)Made available in DSpace on 2012-12-12T10:46:55Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| dc.title.alternative.pt_BR.fl_str_mv |
Infection and Immunity |
| title |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| spellingShingle |
Up-regulation of th1-type responses in mucosal leishmaniasis patients Bacellar, Maria Olívia Amado Ramos |
| title_short |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| title_full |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| title_fullStr |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| title_full_unstemmed |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| title_sort |
Up-regulation of th1-type responses in mucosal leishmaniasis patients |
| author |
Bacellar, Maria Olívia Amado Ramos |
| author_facet |
Bacellar, Maria Olívia Amado Ramos Lessa, Hélio Andrade Schriefer, Nicolaus Albert Borges Machado, Paulo Roberto Lima Jesus, Amélia Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
| author_role |
author |
| author2 |
Lessa, Hélio Andrade Schriefer, Nicolaus Albert Borges Machado, Paulo Roberto Lima Jesus, Amélia Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
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author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bacellar, Maria Olívia Amado Ramos Lessa, Hélio Andrade Schriefer, Nicolaus Albert Borges Machado, Paulo Roberto Lima Jesus, Amélia Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de Bacellar, Maria Olívia Amado Ramos Lessa, Hélio Andrade Schriefer, Nicolaus Albert Borges Machado, Paulo Roberto Lima Jesus, Amélia Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
| description |
Texto completo: acesso restrito. p. 6734–6740 |
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2002 |
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2002 |
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2012-12-12T10:46:55Z |
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info:eu-repo/semantics/publishedVersion |
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0019-9567 |
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v. 70, n. 12 |
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0019-9567 v. 70, n. 12 |
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http://www.repositorio.ufba.br/ri/handle/ri/7437 |
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