Up-regulation of Th1-type responses in mucosal leishmaniasis patients
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/918 |
Resumo: | The cytokine profile produced by peripheral blood mononuclear cells (PBMC) in response to leishmania antigens and the ability of interleukin-10 (IL-10) and transforming growth factor β (TGF-β) to modulate the immune response were evaluated in 21 mucosal leishmaniasis patients. Patients with mucosal disease exhibited increased gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion and decreased IL-10 secretion compared to patients with classical cutaneous leishmaniasis. CD4+ Th1 cells were the main source of IFN-γ and TNF-α production in mucosal leishmaniasis patients. Evaluation of cytokine gene expression in PBMC of these patients showed that there was strong up-regulation of IFN-γ transcripts upon stimulation with leishmania antigen, in contrast to the baseline levels of IL-10 mRNA. IL-10 suppressed IFN-γ production by 48% in cell cultures from cutaneous leishmaniasis patients and by 86% in cell cultures from healthy subjects stimulated with purified protein derivative, whereas in similar conditions IL-10 suppressed IFN-γ production by 19% in cell cultures from mucosal leishmaniasis patients stimulated with leishmania antigen. TGF-β suppressed IFN-γ levels to a greater extent in healthy subjects than in mucosal leishmaniasis and cutaneous leishmaniasis patients. These data indicate that a poorly modulated T-cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines, such as IFN-γ and TNF-α, as well as a decreased ability of IL-10 and TGF-β to modulate this response. These abnormalities may be the basis for the pathological findings observed in this disease. |
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Bacellar, OlíviaLessa, HélioSchriefer, AlbertMachado, Paulo Roberto LimaJesus, Amélia Maria Ribeiro deDutra, Walderez OrnelasGollob, Kenneth JohnCarvalho Filho, Edgar Marcelino de2014-02-19T22:38:40Z2014-02-19T22:38:40Z2002-12BACELLAR, O et al. Up-regulation of Th1-type responses in mucosal leishmaniasis patients. Infection and Immunity, Washington, v. 70, n. 12, dez. 2002. Disponível em: <http://iai.asm.org/content/70/12/6734.long>. Acesso em: 19 fev. 2014.1098-5522https://ri.ufs.br/handle/riufs/918Copyright © by the American Society for MicrobiologyThe cytokine profile produced by peripheral blood mononuclear cells (PBMC) in response to leishmania antigens and the ability of interleukin-10 (IL-10) and transforming growth factor β (TGF-β) to modulate the immune response were evaluated in 21 mucosal leishmaniasis patients. Patients with mucosal disease exhibited increased gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion and decreased IL-10 secretion compared to patients with classical cutaneous leishmaniasis. CD4+ Th1 cells were the main source of IFN-γ and TNF-α production in mucosal leishmaniasis patients. Evaluation of cytokine gene expression in PBMC of these patients showed that there was strong up-regulation of IFN-γ transcripts upon stimulation with leishmania antigen, in contrast to the baseline levels of IL-10 mRNA. IL-10 suppressed IFN-γ production by 48% in cell cultures from cutaneous leishmaniasis patients and by 86% in cell cultures from healthy subjects stimulated with purified protein derivative, whereas in similar conditions IL-10 suppressed IFN-γ production by 19% in cell cultures from mucosal leishmaniasis patients stimulated with leishmania antigen. TGF-β suppressed IFN-γ levels to a greater extent in healthy subjects than in mucosal leishmaniasis and cutaneous leishmaniasis patients. These data indicate that a poorly modulated T-cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines, such as IFN-γ and TNF-α, as well as a decreased ability of IL-10 and TGF-β to modulate this response. These abnormalities may be the basis for the pathological findings observed in this disease.American Society for MicrobiologyLeishmaniose mucosaCitocinasInterleucinaInterleucina 10Up-regulation of Th1-type responses in mucosal leishmaniasis patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILUpRegulationTh1.pdf.jpgUpRegulationTh1.pdf.jpgGenerated Thumbnailimage/jpeg1770https://ri.ufs.br/jspui/bitstream/riufs/918/6/UpRegulationTh1.pdf.jpge90586210695813105d50a3ef824e702MD56ORIGINALUpRegulationTh1.pdfUpRegulationTh1.pdfapplication/pdf203620https://ri.ufs.br/jspui/bitstream/riufs/918/4/UpRegulationTh1.pdf678dbc0113d3679b9845f7278f113ddaMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/918/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTUp-RegulationLeishmaniasis.pdf.txtUp-RegulationLeishmaniasis.pdf.txtExtracted texttext/plain38838https://ri.ufs.br/jspui/bitstream/riufs/918/3/Up-RegulationLeishmaniasis.pdf.txt3ba2a2c663119a00ee145150688e2f07MD53UpRegulationTh1.pdf.txtUpRegulationTh1.pdf.txtExtracted texttext/plain40133https://ri.ufs.br/jspui/bitstream/riufs/918/5/UpRegulationTh1.pdf.txt726f7b9bede8e9ac630bab283a36cb4bMD55riufs/9182018-02-09 19:34:28.267oai:ufs.br:riufs/918Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-02-09T22:34:28Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
title |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
spellingShingle |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients Bacellar, Olívia Leishmaniose mucosa Citocinas Interleucina Interleucina 10 |
title_short |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
title_full |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
title_fullStr |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
title_full_unstemmed |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
title_sort |
Up-regulation of Th1-type responses in mucosal leishmaniasis patients |
author |
Bacellar, Olívia |
author_facet |
Bacellar, Olívia Lessa, Hélio Schriefer, Albert Machado, Paulo Roberto Lima Jesus, Amélia Maria Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
author_role |
author |
author2 |
Lessa, Hélio Schriefer, Albert Machado, Paulo Roberto Lima Jesus, Amélia Maria Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bacellar, Olívia Lessa, Hélio Schriefer, Albert Machado, Paulo Roberto Lima Jesus, Amélia Maria Ribeiro de Dutra, Walderez Ornelas Gollob, Kenneth John Carvalho Filho, Edgar Marcelino de |
dc.subject.por.fl_str_mv |
Leishmaniose mucosa Citocinas Interleucina Interleucina 10 |
topic |
Leishmaniose mucosa Citocinas Interleucina Interleucina 10 |
description |
The cytokine profile produced by peripheral blood mononuclear cells (PBMC) in response to leishmania antigens and the ability of interleukin-10 (IL-10) and transforming growth factor β (TGF-β) to modulate the immune response were evaluated in 21 mucosal leishmaniasis patients. Patients with mucosal disease exhibited increased gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion and decreased IL-10 secretion compared to patients with classical cutaneous leishmaniasis. CD4+ Th1 cells were the main source of IFN-γ and TNF-α production in mucosal leishmaniasis patients. Evaluation of cytokine gene expression in PBMC of these patients showed that there was strong up-regulation of IFN-γ transcripts upon stimulation with leishmania antigen, in contrast to the baseline levels of IL-10 mRNA. IL-10 suppressed IFN-γ production by 48% in cell cultures from cutaneous leishmaniasis patients and by 86% in cell cultures from healthy subjects stimulated with purified protein derivative, whereas in similar conditions IL-10 suppressed IFN-γ production by 19% in cell cultures from mucosal leishmaniasis patients stimulated with leishmania antigen. TGF-β suppressed IFN-γ levels to a greater extent in healthy subjects than in mucosal leishmaniasis and cutaneous leishmaniasis patients. These data indicate that a poorly modulated T-cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines, such as IFN-γ and TNF-α, as well as a decreased ability of IL-10 and TGF-β to modulate this response. These abnormalities may be the basis for the pathological findings observed in this disease. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002-12 |
dc.date.accessioned.fl_str_mv |
2014-02-19T22:38:40Z |
dc.date.available.fl_str_mv |
2014-02-19T22:38:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BACELLAR, O et al. Up-regulation of Th1-type responses in mucosal leishmaniasis patients. Infection and Immunity, Washington, v. 70, n. 12, dez. 2002. Disponível em: <http://iai.asm.org/content/70/12/6734.long>. Acesso em: 19 fev. 2014. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/918 |
dc.identifier.issn.none.fl_str_mv |
1098-5522 |
dc.identifier.license.pt_BR.fl_str_mv |
Copyright © by the American Society for Microbiology |
identifier_str_mv |
BACELLAR, O et al. Up-regulation of Th1-type responses in mucosal leishmaniasis patients. Infection and Immunity, Washington, v. 70, n. 12, dez. 2002. Disponível em: <http://iai.asm.org/content/70/12/6734.long>. Acesso em: 19 fev. 2014. 1098-5522 Copyright © by the American Society for Microbiology |
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https://ri.ufs.br/handle/riufs/918 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
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Universidade Federal de Sergipe (UFS) |
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UFS |
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