Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista ciência agronômica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100090 |
Resumo: | ABSTRACT The aim of this study was to evaluate different concentrations of two cytokinins in the in vitro propagation of the medicinal plant Hyptis marrubioides Epl. and to analyse the volatile fraction of the compounds. Nodal segments of plants were inoculated onto Murashige and Skoog (MS) solid medium with different concentrations of benzylaminopurine (BAP) or tidiazuron (TDZ) under a 16/8 h light/dark cycle at 25 ± 2 °C. After 45 days growth, the plants were evaluated for the number and length of the shoots and for shoot dry matter. The volatile constituents were analysed by headspace-GC/MS. The greatest number of shoots was obtained with 1.0 mg L-1 TDZ in the MS medium. The greatest values for shoot length and dry matter were obtained with BAP. Twenty-seven compounds were characterised as constituents of the essential oil of H. marrubioides. The major compounds of the volatile fraction were sabinene, α-thujone, β- thujone, α-copaene, β-caryophyllene, γ-gurjunene and γ-himachalene. The types and concentrations of the growth regulators influenced accumulation of the volatile fraction. Quantitative changes in the monoterpenes and sesquiterpenes in the volatile fraction of the plants were also seen in response to the type of growth regulator added to the culture medium. |
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Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioidesGrowth regulatorsProliferationTerpenesMedicinal plantHyptis smarrubioidesABSTRACT The aim of this study was to evaluate different concentrations of two cytokinins in the in vitro propagation of the medicinal plant Hyptis marrubioides Epl. and to analyse the volatile fraction of the compounds. Nodal segments of plants were inoculated onto Murashige and Skoog (MS) solid medium with different concentrations of benzylaminopurine (BAP) or tidiazuron (TDZ) under a 16/8 h light/dark cycle at 25 ± 2 °C. After 45 days growth, the plants were evaluated for the number and length of the shoots and for shoot dry matter. The volatile constituents were analysed by headspace-GC/MS. The greatest number of shoots was obtained with 1.0 mg L-1 TDZ in the MS medium. The greatest values for shoot length and dry matter were obtained with BAP. Twenty-seven compounds were characterised as constituents of the essential oil of H. marrubioides. The major compounds of the volatile fraction were sabinene, α-thujone, β- thujone, α-copaene, β-caryophyllene, γ-gurjunene and γ-himachalene. The types and concentrations of the growth regulators influenced accumulation of the volatile fraction. Quantitative changes in the monoterpenes and sesquiterpenes in the volatile fraction of the plants were also seen in response to the type of growth regulator added to the culture medium.Universidade Federal do Ceará2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100090Revista Ciência Agronômica v.50 n.1 2019reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.5935/1806-6690.20190011info:eu-repo/semantics/openAccessRodrigues,Mariane AparecidaBertolucci,Suzan Kelly VilelaAlvarenga,Ivan Caldeira AlmeidaSilva,Sâmia TorresCarvalho,Alexandre Alves dePinto,José Eduardo Brasil Pereiraeng2018-11-07T00:00:00Zoai:scielo:S1806-66902019000100090Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2018-11-07T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
title |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
spellingShingle |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides Rodrigues,Mariane Aparecida Growth regulators Proliferation Terpenes Medicinal plant Hyptis smarrubioides |
title_short |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
title_full |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
title_fullStr |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
title_full_unstemmed |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
title_sort |
Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides |
author |
Rodrigues,Mariane Aparecida |
author_facet |
Rodrigues,Mariane Aparecida Bertolucci,Suzan Kelly Vilela Alvarenga,Ivan Caldeira Almeida Silva,Sâmia Torres Carvalho,Alexandre Alves de Pinto,José Eduardo Brasil Pereira |
author_role |
author |
author2 |
Bertolucci,Suzan Kelly Vilela Alvarenga,Ivan Caldeira Almeida Silva,Sâmia Torres Carvalho,Alexandre Alves de Pinto,José Eduardo Brasil Pereira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Mariane Aparecida Bertolucci,Suzan Kelly Vilela Alvarenga,Ivan Caldeira Almeida Silva,Sâmia Torres Carvalho,Alexandre Alves de Pinto,José Eduardo Brasil Pereira |
dc.subject.por.fl_str_mv |
Growth regulators Proliferation Terpenes Medicinal plant Hyptis smarrubioides |
topic |
Growth regulators Proliferation Terpenes Medicinal plant Hyptis smarrubioides |
description |
ABSTRACT The aim of this study was to evaluate different concentrations of two cytokinins in the in vitro propagation of the medicinal plant Hyptis marrubioides Epl. and to analyse the volatile fraction of the compounds. Nodal segments of plants were inoculated onto Murashige and Skoog (MS) solid medium with different concentrations of benzylaminopurine (BAP) or tidiazuron (TDZ) under a 16/8 h light/dark cycle at 25 ± 2 °C. After 45 days growth, the plants were evaluated for the number and length of the shoots and for shoot dry matter. The volatile constituents were analysed by headspace-GC/MS. The greatest number of shoots was obtained with 1.0 mg L-1 TDZ in the MS medium. The greatest values for shoot length and dry matter were obtained with BAP. Twenty-seven compounds were characterised as constituents of the essential oil of H. marrubioides. The major compounds of the volatile fraction were sabinene, α-thujone, β- thujone, α-copaene, β-caryophyllene, γ-gurjunene and γ-himachalene. The types and concentrations of the growth regulators influenced accumulation of the volatile fraction. Quantitative changes in the monoterpenes and sesquiterpenes in the volatile fraction of the plants were also seen in response to the type of growth regulator added to the culture medium. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100090 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1806-6690.20190011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal do Ceará |
publisher.none.fl_str_mv |
Universidade Federal do Ceará |
dc.source.none.fl_str_mv |
Revista Ciência Agronômica v.50 n.1 2019 reponame:Revista ciência agronômica (Online) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista ciência agronômica (Online) |
collection |
Revista ciência agronômica (Online) |
repository.name.fl_str_mv |
Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
||alekdutra@ufc.br|| ccarev@ufc.br |
_version_ |
1750297489387290624 |