Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Pinho, Flaviane Alves de; UNIR/EMBRAPA
Data de Publicação: 2020
Outros Autores: Alves, Geórgia Brenda Barros; Universidade Federal do Piauí, Cruz, Maria do Socorro Pires e; Universidade Federal do Piaui
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista brasileira de higiene e sanidade animal
Texto Completo: http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/568
Resumo: Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.
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spelling Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasisLeishmania, canine, CD4, CD8.Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.Revista Brasileira de Higiene e Sanidade Animal2020-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/56810.5935/rbhsa.v14i2.568Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 142-157reponame:Revista brasileira de higiene e sanidade animalinstname:Universidade Federal do Ceará (UFC)instacron:UFCporhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/568/2849Pinho, Flaviane Alves de; UNIR/EMBRAPAAlves, Geórgia Brenda Barros; Universidade Federal do PiauíCruz, Maria do Socorro Pires e; Universidade Federal do Piauiinfo:eu-repo/semantics/openAccess2021-03-24T14:18:45Zoai:ojs.www.higieneanimal.ufc.br:article/568Revistahttp://www.higieneanimal.ufc.br/PUBhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/oaiwesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br1981-29651981-2965opendoar:2021-03-24T14:18:45Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
spellingShingle Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
Pinho, Flaviane Alves de; UNIR/EMBRAPA
Leishmania, canine, CD4, CD8.
title_short Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title_full Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title_fullStr Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title_full_unstemmed Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title_sort Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
author Pinho, Flaviane Alves de; UNIR/EMBRAPA
author_facet Pinho, Flaviane Alves de; UNIR/EMBRAPA
Alves, Geórgia Brenda Barros; Universidade Federal do Piauí
Cruz, Maria do Socorro Pires e; Universidade Federal do Piaui
author_role author
author2 Alves, Geórgia Brenda Barros; Universidade Federal do Piauí
Cruz, Maria do Socorro Pires e; Universidade Federal do Piaui
author2_role author
author
dc.contributor.author.fl_str_mv Pinho, Flaviane Alves de; UNIR/EMBRAPA
Alves, Geórgia Brenda Barros; Universidade Federal do Piauí
Cruz, Maria do Socorro Pires e; Universidade Federal do Piaui
dc.subject.por.fl_str_mv Leishmania, canine, CD4, CD8.
topic Leishmania, canine, CD4, CD8.
description Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-24
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dc.identifier.uri.fl_str_mv http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/568
10.5935/rbhsa.v14i2.568
url http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/568
identifier_str_mv 10.5935/rbhsa.v14i2.568
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/568/2849
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dc.publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
dc.source.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 142-157
reponame:Revista brasileira de higiene e sanidade animal
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
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reponame_str Revista brasileira de higiene e sanidade animal
collection Revista brasileira de higiene e sanidade animal
repository.name.fl_str_mv Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv wesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br
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