Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral

Detalhes bibliográficos
Autor(a) principal: Pinho, Flaviane Alves de
Data de Publicação: 2020
Outros Autores: Alves, Geórgia Brenda Barros, Cruz, Maria do Socorro Pires e
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/53627
Resumo: Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.
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spelling Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceralCellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasisLeishmaniaCaninoCD4CD8Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.Leishmaniose visceral canina (LVC) é uma zoonose causada por Leishmania (Leishmania) infantum chagasi no continente Americano. Poucos estudos demonstraram as características imunopatológicas em linfonodo poplíteo e fígado de cães infectados em área endêmica de Teresina, Piauí, Brasil. Assim, nós avaliamos as alterações histopatológicas e a presença de células T CD4+ e CD8+ em linfonodo poplíteo e fígado de nove cães com manifestações clínicas (G1) e sete cães sem manifestações clínicas (G2) naturalmente infectados com L. (L.) infantum chagasi. Fragmentos de linfonodo poplíteo e fígado foram obtidos para análise histopatológica e imunoistoquímica. As principais manifestações clínicas foram linfadenopatia (80%) e lesões de pele (70%). Na análise histopatológica do linfonodo foi observada uma depleção de células da região cortical. Não havia diferença significante entre os grupos entre células T CD4+ e CD8+ mas havia uma correlação positiva entre essas células em G1 e G2. No fígado, o infiltrado inflamatório era focal consistindo de células mononucleares nas regiões periportal e intralobular, em ambos os grupos. O infiltrado inflamatório era positivo na imunomarcação para células T CD4 e CD8, mas sem diferença significante entre os grupos. Entretanto, havia uma correlação positiva entre as células T CD4+ e CD8+ no G1. Na análise da imunomarcação também mostrou mais amastigotas e antígenos de Leishmania no fígado e linfonodo poplíteo em G1 em relação ao G2. O papel das células T CD4+ e CD8+ parece não estar relacionada ao estado clínico dos cães com LVC.Revista Brasileira de Higiene e Sanidade Animal2020-08-25T12:09:10Z2020-08-25T12:09:10Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPINHO, Flaviane Alves de; ALVES, Geórgia Brenda Barros; CRUZ, Maria do Socorro Pires e. Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral. Revista Brasileira de Higiene e Sanidade Animal, Fortaleza, v. 14, n. 2, p. 142-157, 2020.1981-2965http://www.repositorio.ufc.br/handle/riufc/53627Pinho, Flaviane Alves deAlves, Geórgia Brenda BarrosCruz, Maria do Socorro Pires einfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-06T12:24:39Zoai:repositorio.ufc.br:riufc/53627Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:22:24.886753Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
Cellular immune response in popliteal lymph nodes and liver of dogs with visceral leishmaniasis
title Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
spellingShingle Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
Pinho, Flaviane Alves de
Leishmania
Canino
CD4
CD8
title_short Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
title_full Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
title_fullStr Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
title_full_unstemmed Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
title_sort Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral
author Pinho, Flaviane Alves de
author_facet Pinho, Flaviane Alves de
Alves, Geórgia Brenda Barros
Cruz, Maria do Socorro Pires e
author_role author
author2 Alves, Geórgia Brenda Barros
Cruz, Maria do Socorro Pires e
author2_role author
author
dc.contributor.author.fl_str_mv Pinho, Flaviane Alves de
Alves, Geórgia Brenda Barros
Cruz, Maria do Socorro Pires e
dc.subject.por.fl_str_mv Leishmania
Canino
CD4
CD8
topic Leishmania
Canino
CD4
CD8
description Canine visceral leishmaniasis (CVL) is a zoonosis caused by Leishmania (Leishmania) infantum chagasi in the American continent. There are few studies detailing the immunopathological characteristics in lymph nodes and liver in infected dogs in endemic area from Teresina city, Piauí State, Brazil. Thus, we have evaluated histopathological changes and the presence of CD4+ and CD8+ T cells in popliteal lymph nodes and liver of nine dogs with clinical manifestation (G1) and seven dogs without clinical manifestation (G2) naturally infected with L. (L.) infantum chagasi. Histopathological and immunohistochemical analysis were performed on samples liver and lymph nodes tissues. The important clinical changes were lymphadenopathy (80%) and skin lesions (70%). Histopathological analysis of the popliteal lymph node revealed higher depletion cell of the paracortical region. The number of CD4+ and CD8+ T cells was no significant between the groups but there was positive correlation between CD4+ and CD8+ T cells in G1 and G2. In the liver, the inflammatory infiltrate was focal consisting of mononuclear cell in the periportal and intralobular regions, in both groups. Inflammatory infiltrate of mononuclear cells showed positive staining for CD4 and CD8 T cells but there was no significant difference between groups. However, there was a positive correlation between CD4+ and CD8+ T cells in the G1. Immunoperoxidase analysis also revealed more amastigotes and antigen of Leishmania in G1 in liver and lymph nodes than in G2. The role of CD4+ and CD8+ T cells seems is not related to the clinical status of dogs with CVL.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-25T12:09:10Z
2020-08-25T12:09:10Z
2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PINHO, Flaviane Alves de; ALVES, Geórgia Brenda Barros; CRUZ, Maria do Socorro Pires e. Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral. Revista Brasileira de Higiene e Sanidade Animal, Fortaleza, v. 14, n. 2, p. 142-157, 2020.
1981-2965
http://www.repositorio.ufc.br/handle/riufc/53627
identifier_str_mv PINHO, Flaviane Alves de; ALVES, Geórgia Brenda Barros; CRUZ, Maria do Socorro Pires e. Resposta imunológica celular em linfonodo poplíteo e fígado de cães com leishmaniose visceral. Revista Brasileira de Higiene e Sanidade Animal, Fortaleza, v. 14, n. 2, p. 142-157, 2020.
1981-2965
url http://www.repositorio.ufc.br/handle/riufc/53627
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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