L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis

Detalhes bibliográficos
Autor(a) principal: Morais, Gustavo J. S.
Data de Publicação: 2015
Outros Autores: Orzáez, M., Jorge, Roberta J. B., Bincoletto, Claudia, Toyama, Marcos H., Monteiro, Helena S. A., Smaili, Soraya S., Pérez-Payá, Enrique, Martins, Alice M. C., Pereira, Gustavo J. S.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
dARK ID: ark:/83112/001300001d8bf
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/17768
Resumo: Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.
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spelling L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosisBothropsNecroseNefropatiasAcute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.Plos One2016-06-17T12:51:12Z2016-06-17T12:51:12Z2015-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015.1932-6203http://www.repositorio.ufc.br/handle/riufc/17768ark:/83112/001300001d8bfMorais, Gustavo J. S.Orzáez, M.Jorge, Roberta J. B.Bincoletto, ClaudiaToyama, Marcos H.Monteiro, Helena S. A.Smaili, Soraya S.Pérez-Payá, EnriqueMartins, Alice M. C.Pereira, Gustavo J. S.porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-18T17:29:00Zoai:repositorio.ufc.br:riufc/17768Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:44:21.228845Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
title L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
spellingShingle L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
Morais, Gustavo J. S.
Bothrops
Necrose
Nefropatias
title_short L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
title_full L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
title_fullStr L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
title_full_unstemmed L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
title_sort L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
author Morais, Gustavo J. S.
author_facet Morais, Gustavo J. S.
Orzáez, M.
Jorge, Roberta J. B.
Bincoletto, Claudia
Toyama, Marcos H.
Monteiro, Helena S. A.
Smaili, Soraya S.
Pérez-Payá, Enrique
Martins, Alice M. C.
Pereira, Gustavo J. S.
author_role author
author2 Orzáez, M.
Jorge, Roberta J. B.
Bincoletto, Claudia
Toyama, Marcos H.
Monteiro, Helena S. A.
Smaili, Soraya S.
Pérez-Payá, Enrique
Martins, Alice M. C.
Pereira, Gustavo J. S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais, Gustavo J. S.
Orzáez, M.
Jorge, Roberta J. B.
Bincoletto, Claudia
Toyama, Marcos H.
Monteiro, Helena S. A.
Smaili, Soraya S.
Pérez-Payá, Enrique
Martins, Alice M. C.
Pereira, Gustavo J. S.
dc.subject.por.fl_str_mv Bothrops
Necrose
Nefropatias
topic Bothrops
Necrose
Nefropatias
description Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2016-06-17T12:51:12Z
2016-06-17T12:51:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015.
1932-6203
http://www.repositorio.ufc.br/handle/riufc/17768
dc.identifier.dark.fl_str_mv ark:/83112/001300001d8bf
identifier_str_mv MORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015.
1932-6203
ark:/83112/001300001d8bf
url http://www.repositorio.ufc.br/handle/riufc/17768
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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