L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
dARK ID: | ark:/83112/001300001d8bf |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/17768 |
Resumo: | Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney. |
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L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosisBothropsNecroseNefropatiasAcute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.Plos One2016-06-17T12:51:12Z2016-06-17T12:51:12Z2015-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015.1932-6203http://www.repositorio.ufc.br/handle/riufc/17768ark:/83112/001300001d8bfMorais, Gustavo J. S.Orzáez, M.Jorge, Roberta J. B.Bincoletto, ClaudiaToyama, Marcos H.Monteiro, Helena S. A.Smaili, Soraya S.Pérez-Payá, EnriqueMartins, Alice M. C.Pereira, Gustavo J. S.porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-18T17:29:00Zoai:repositorio.ufc.br:riufc/17768Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:44:21.228845Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
title |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
spellingShingle |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis Morais, Gustavo J. S. Bothrops Necrose Nefropatias |
title_short |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
title_full |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
title_fullStr |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
title_full_unstemmed |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
title_sort |
L-Aminoacid oxidase from Bothropsleucurus Venom induces nephrotoxicity via apoptosis and necrosis |
author |
Morais, Gustavo J. S. |
author_facet |
Morais, Gustavo J. S. Orzáez, M. Jorge, Roberta J. B. Bincoletto, Claudia Toyama, Marcos H. Monteiro, Helena S. A. Smaili, Soraya S. Pérez-Payá, Enrique Martins, Alice M. C. Pereira, Gustavo J. S. |
author_role |
author |
author2 |
Orzáez, M. Jorge, Roberta J. B. Bincoletto, Claudia Toyama, Marcos H. Monteiro, Helena S. A. Smaili, Soraya S. Pérez-Payá, Enrique Martins, Alice M. C. Pereira, Gustavo J. S. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Morais, Gustavo J. S. Orzáez, M. Jorge, Roberta J. B. Bincoletto, Claudia Toyama, Marcos H. Monteiro, Helena S. A. Smaili, Soraya S. Pérez-Payá, Enrique Martins, Alice M. C. Pereira, Gustavo J. S. |
dc.subject.por.fl_str_mv |
Bothrops Necrose Nefropatias |
topic |
Bothrops Necrose Nefropatias |
description |
Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B . leucurus venom called L-aminoacid oxidase (LAAO- Bl ) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO- Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induc- tion was accompanied by Ca 2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO- Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO- Bl (10 μ g/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO- Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO- Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07 2016-06-17T12:51:12Z 2016-06-17T12:51:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015. 1932-6203 http://www.repositorio.ufc.br/handle/riufc/17768 |
dc.identifier.dark.fl_str_mv |
ark:/83112/001300001d8bf |
identifier_str_mv |
MORAIS, I. C. O. et al. L-Aminoacid oxidase from Bothropsleucurus venom induces nephrotoxicity via apoptosis and necrosis. Plos One, San Francisco, v. 10, p. 1-14, jul. 2015. 1932-6203 ark:/83112/001300001d8bf |
url |
http://www.repositorio.ufc.br/handle/riufc/17768 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Plos One |
publisher.none.fl_str_mv |
Plos One |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1818373947088961536 |