Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria

Detalhes bibliográficos
Autor(a) principal: Lima, Hilania Valéria Doudou
Data de Publicação: 2020
Outros Autores: Cavalcante, Carolina Sidrim de Paula, Rádis-Baptista, Gandhi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/59683
Resumo: D. quadriceps comprises a rich blend of bioactive peptides that includes structures related to at least five classes of antimicrobial peptides. In the present study, two representative synthetic peptides, sDq-2562 and sDq-3162, belonging to the ponericin-like dinoponeratoxin family, were evaluated for their microbicide activity against antibiotic-resistant bacteria. The most effective peptide, the 28-residue sDq-3162 displayed a significant bacteriostatic and bactericidal effect with minimal inhibitory concentrations (MICs) between 5 μM and 10 μM (15.6 μg mL 1 and 31.2 μg mL 1), according to the strain of drug-resistant bacteria tested. In combination with conventional antibiotics, sDq-3162 displayed in vitro synergistic effects, reducing the MICs of antibiotics for more than 2-log against clinical isolates of carbapenem-resistant Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa, with low cytotoxicity to human erythrocytes, in vitro. Since the development of molecules to circumvent the spread of antibiotic-resistant bacteria is demanding, ant venom peptides arise as useful molecular resources to contribute with the antimicrobial arsenal and therapeutic strategies to fight clinically relevant microbial infections.
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spelling Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactériaAntimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactériaPeptídeosCitotóxicosAntimicrobianosD. quadriceps comprises a rich blend of bioactive peptides that includes structures related to at least five classes of antimicrobial peptides. In the present study, two representative synthetic peptides, sDq-2562 and sDq-3162, belonging to the ponericin-like dinoponeratoxin family, were evaluated for their microbicide activity against antibiotic-resistant bacteria. The most effective peptide, the 28-residue sDq-3162 displayed a significant bacteriostatic and bactericidal effect with minimal inhibitory concentrations (MICs) between 5 μM and 10 μM (15.6 μg mL 1 and 31.2 μg mL 1), according to the strain of drug-resistant bacteria tested. In combination with conventional antibiotics, sDq-3162 displayed in vitro synergistic effects, reducing the MICs of antibiotics for more than 2-log against clinical isolates of carbapenem-resistant Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa, with low cytotoxicity to human erythrocytes, in vitro. Since the development of molecules to circumvent the spread of antibiotic-resistant bacteria is demanding, ant venom peptides arise as useful molecular resources to contribute with the antimicrobial arsenal and therapeutic strategies to fight clinically relevant microbial infections.Toxicon2021-07-21T19:26:43Z2021-07-21T19:26:43Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLIMA, Hilania Valéria Doudou; CAVALCANTE, Carolina Sidrim de Paula; RÁDIS-BAPTISTA, Gandhi. Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria.Toxicon, v. 187, 2020.Disponível em: https://doi.org/10.1016/j.toxicon.2020.08.015. Acesso em: 21 jul. 20210041-0101http://www.repositorio.ufc.br/handle/riufc/59683Lima, Hilania Valéria DoudouCavalcante, Carolina Sidrim de PaulaRádis-Baptista, Gandhiengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-12-17T11:49:20Zoai:repositorio.ufc.br:riufc/59683Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:34:56.574527Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
title Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
spellingShingle Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
Lima, Hilania Valéria Doudou
Peptídeos
Citotóxicos
Antimicrobianos
title_short Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
title_full Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
title_fullStr Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
title_full_unstemmed Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
title_sort Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria
author Lima, Hilania Valéria Doudou
author_facet Lima, Hilania Valéria Doudou
Cavalcante, Carolina Sidrim de Paula
Rádis-Baptista, Gandhi
author_role author
author2 Cavalcante, Carolina Sidrim de Paula
Rádis-Baptista, Gandhi
author2_role author
author
dc.contributor.author.fl_str_mv Lima, Hilania Valéria Doudou
Cavalcante, Carolina Sidrim de Paula
Rádis-Baptista, Gandhi
dc.subject.por.fl_str_mv Peptídeos
Citotóxicos
Antimicrobianos
topic Peptídeos
Citotóxicos
Antimicrobianos
description D. quadriceps comprises a rich blend of bioactive peptides that includes structures related to at least five classes of antimicrobial peptides. In the present study, two representative synthetic peptides, sDq-2562 and sDq-3162, belonging to the ponericin-like dinoponeratoxin family, were evaluated for their microbicide activity against antibiotic-resistant bacteria. The most effective peptide, the 28-residue sDq-3162 displayed a significant bacteriostatic and bactericidal effect with minimal inhibitory concentrations (MICs) between 5 μM and 10 μM (15.6 μg mL 1 and 31.2 μg mL 1), according to the strain of drug-resistant bacteria tested. In combination with conventional antibiotics, sDq-3162 displayed in vitro synergistic effects, reducing the MICs of antibiotics for more than 2-log against clinical isolates of carbapenem-resistant Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa, with low cytotoxicity to human erythrocytes, in vitro. Since the development of molecules to circumvent the spread of antibiotic-resistant bacteria is demanding, ant venom peptides arise as useful molecular resources to contribute with the antimicrobial arsenal and therapeutic strategies to fight clinically relevant microbial infections.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-07-21T19:26:43Z
2021-07-21T19:26:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LIMA, Hilania Valéria Doudou; CAVALCANTE, Carolina Sidrim de Paula; RÁDIS-BAPTISTA, Gandhi. Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria.Toxicon, v. 187, 2020.Disponível em: https://doi.org/10.1016/j.toxicon.2020.08.015. Acesso em: 21 jul. 2021
0041-0101
http://www.repositorio.ufc.br/handle/riufc/59683
identifier_str_mv LIMA, Hilania Valéria Doudou; CAVALCANTE, Carolina Sidrim de Paula; RÁDIS-BAPTISTA, Gandhi. Antimicrobial activity of synthetic Dq-3162, a 28-residue ponericin G-like dinoponeratoxin from the giant ant Dinoponera quadriceps venom, against carbapenem-resistant bactéria.Toxicon, v. 187, 2020.Disponível em: https://doi.org/10.1016/j.toxicon.2020.08.015. Acesso em: 21 jul. 2021
0041-0101
url http://www.repositorio.ufc.br/handle/riufc/59683
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Toxicon
publisher.none.fl_str_mv Toxicon
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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