Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=5285 |
Resumo: | Dental Caries is an infectious disease naturally multi-factorial that begins in the childhood, able to spread its intensity and prevalence according to the mouth ambient conditions of each host. The risk of the patients to develop, in some time of their lives, carious lesions can be measured through a complex analysis using health determinant factors and other specific factors of the health-disease process. Since the dental caries forward diagnostic as well as the review of the auxiliary determinant factors of the dental caries danger evaluation, the treatment becomes simpler, less invasive and costing lower. It has been quite broad-casted lately the use of lectins to diagnose and to prevent some diseases. When dealing with dental caries disease, through many years this strategy has been explored still inceptively. Looking through this perspective, the goal of this trial was to investigate the biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry. It is about an explanatory-experimental study of quantitative approach through the health questionnaire and data statistics analysis. The research makes reference to some comparative analysis of salivary tests and the cariogenic activity in twelve year-old students and it searches to evaluate the biotechnological potentialities of the lectin from the red marine alga Hypnea musciformis (HML) in the diagnose and prevention of the dental caries. The concentration area of this trial was the macromolecules/ lectin biotechnological application. In the present trial were compared obtained results through the application of the conventional clinical exams to dental caries and salivary tests of dental caries activity pointed by the CARIOGRAMÂ program in association with lab analysis using specific lectins. From this trial, it has been concluded that, about lectins as a biotechnological potential to prevention and diagnose of the dental caries, it has been noticed that there is recognition of resident glycoconjugate on pacifier saliva of high and low risk observed in immunofluorescence pattern. The results showed that the tested lectin has the capacity to intervene on the bacterial growth in a negative way, it is being distinguished statistically (p<0,01) in relation to control group of saline and BSA. However, inhibition studies of the microbial biofilms using the HML are being programmed to evaluate the using possibility of these protein as biotech input to be used for dental caries prevention. It could be said also that the obtained results by the use of HML in the antimicrobial activity were promising, showing that this lectin has presented antimicrobial activity even on very small amounts. As to the use of these protein in the biofilm formation and developing there was no characteristic pattern. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBiotechnological potential of the red marine alga Hypnea musciformis (HML) applied to DentistryAvaliaÃÃo das potencialidades biotecnolÃgicas de lectinas vegetais no diagnÃstico e prevenÃÃo de cÃrie dentÃria2010-02-25Edson Holanda Teixeira71755985304http://lattes.cnpq.br/8800103074322967 Euclides Gomes Parente Filho 26338440300http://lattes.cnpq.br/8405955314901291AndrÃa Silvia Walter de Aguiar31761500325http://lattes.cnpq.br/0341117842153741 75733463368http://lattes.cnpq.br/1556885629243172 FÃbio Solon TajraUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Biotecnologia (Campus da UFC em Sobral-CE)UFCBRCÃrie DentÃria Lectinas Potencial BiotecnolÃgicoDental Caries Lectins Biotechnological PotentialitiesBIOQUIMICADental Caries is an infectious disease naturally multi-factorial that begins in the childhood, able to spread its intensity and prevalence according to the mouth ambient conditions of each host. The risk of the patients to develop, in some time of their lives, carious lesions can be measured through a complex analysis using health determinant factors and other specific factors of the health-disease process. Since the dental caries forward diagnostic as well as the review of the auxiliary determinant factors of the dental caries danger evaluation, the treatment becomes simpler, less invasive and costing lower. It has been quite broad-casted lately the use of lectins to diagnose and to prevent some diseases. When dealing with dental caries disease, through many years this strategy has been explored still inceptively. Looking through this perspective, the goal of this trial was to investigate the biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry. It is about an explanatory-experimental study of quantitative approach through the health questionnaire and data statistics analysis. The research makes reference to some comparative analysis of salivary tests and the cariogenic activity in twelve year-old students and it searches to evaluate the biotechnological potentialities of the lectin from the red marine alga Hypnea musciformis (HML) in the diagnose and prevention of the dental caries. The concentration area of this trial was the macromolecules/ lectin biotechnological application. In the present trial were compared obtained results through the application of the conventional clinical exams to dental caries and salivary tests of dental caries activity pointed by the CARIOGRAM program in association with lab analysis using specific lectins. From this trial, it has been concluded that, about lectins as a biotechnological potential to prevention and diagnose of the dental caries, it has been noticed that there is recognition of resident glycoconjugate on pacifier saliva of high and low risk observed in immunofluorescence pattern. The results showed that the tested lectin has the capacity to intervene on the bacterial growth in a negative way, it is being distinguished statistically (p<0,01) in relation to control group of saline and BSA. However, inhibition studies of the microbial biofilms using the HML are being programmed to evaluate the using possibility of these protein as biotech input to be used for dental caries prevention. It could be said also that the obtained results by the use of HML in the antimicrobial activity were promising, showing that this lectin has presented antimicrobial activity even on very small amounts. As to the use of these protein in the biofilm formation and developing there was no characteristic pattern. A cÃrie à uma doenÃa infecciosa de natureza multifatorial que se inicia na infÃncia, podendo aumentar de intensidade e prevalÃncia de acordo com as condiÃÃes do ambiente bucal de cada hospedeiro. O risco do paciente em desenvolver, em algum perÃodo de seu ciclo de vida, lesÃes cariosas pode ser medido atravÃs de uma anÃlise complexa que utiliza os fatores determinantes da saÃde e outros fatores especÃficos do processo saÃde-doenÃa. A partir do diagnÃstico precoce da doenÃa cÃrie, assim como anÃlise dos fatores determinantes auxiliares da avaliaÃÃo do risco de cÃrie, o tratamento torna-se mais simples, menos invasivo e de menor custo. Tem sido bastante difundida ultimamente a utilizaÃÃo de lectinas para diagnÃstico e prevenÃÃo de algumas doenÃas. Em se tratando da doenÃa cÃrie, ao longo de muitos anos, essa estratÃgia tem sido explorada ainda de forma incipiente. Nesta perspectiva, o objetivo deste trabalho foi investigar as potencialidades biotecnolÃgicas da lectina de alga marinha da espÃcie de Hypnea musciformis (HML) aplicadas à odontologia. Trata-se de um estudo explicativo-experimental de abordagem quantitativa atravÃs de questionÃrio de saÃde e anÃlises estatÃsticas de dados. A pesquisa faz referÃncia à uma anÃlise comparativa de testes salivares e atividade cariogÃnica em escolares de 12 anos de idade e a busca avaliar as potencialidades biotecnolÃgicas da lectina de alga marinha da espÃcie de Hypnea musciformis (HML) no diagnÃstico e prevenÃÃo de cÃrie dentÃria. A Ãrea de concentraÃÃo deste estudo MacromolÃtulas / AplicaÃÃo BiotecnolÃgica de Lectinas. No presente estudo, foram comparados os resultados obtidos atravÃs da aplicaÃÃo de exame clÃnico convencional para cÃrie e testes salivares de atividade cariogÃnica apontados pelo programa CARIOGRAM em associaÃÃo com anÃlises laboratoriais com a utilizaÃÃo de lectinas especÃficas. A partir deste estudo, concluiu-se que, em se tratando do uso de lectinas como potencial biotecnolÃgico para a prevenÃÃo e diagnÃstico de cÃrie dentÃria, percebeu-se que hà reconhecimento de glicoconjugados presentes na saliva de pacientes de alto e baixo risco observados em padrÃo de fluorescÃncia. Os resultados demonstram que a lectina testada tem a capacidade de interferir no crescimento bacteriano de forma negativa, sendo estatisticamente significante (p<0,01) em relaÃÃo aos grupos controle de salina e BSA. Entretanto estudos de inibiÃÃo da formaÃÃo de biofilmes microbianos utilizando a HML estÃo sendo programados, para assim avaliar a possibilidade de utilizaÃÃo dessas proteÃnas como insumos biotecnolÃgicos a ser utilizados para prevenÃÃo da cÃrie dentÃria. Pode-se ainda afirmar que os resultados obtidos pelo uso de HML na atividade antibacteriana foram promissores, mostrando que esta lectina apresentou atividade antibacteriana mesmo em quantidades muito pequenas. Quanto ao uso destas proteÃnas na formaÃÃo e desenvolvimento de biofilmes, nÃo houve padrÃo caracterÃstico.FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicohttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=5285application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:18:22Zmail@mail.com - |
dc.title.en.fl_str_mv |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
dc.title.alternative.pt.fl_str_mv |
AvaliaÃÃo das potencialidades biotecnolÃgicas de lectinas vegetais no diagnÃstico e prevenÃÃo de cÃrie dentÃria |
title |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
spellingShingle |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry FÃbio Solon Tajra CÃrie DentÃria Lectinas Potencial BiotecnolÃgico Dental Caries Lectins Biotechnological Potentialities BIOQUIMICA |
title_short |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
title_full |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
title_fullStr |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
title_full_unstemmed |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
title_sort |
Biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry |
author |
FÃbio Solon Tajra |
author_facet |
FÃbio Solon Tajra |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Edson Holanda Teixeira |
dc.contributor.advisor1ID.fl_str_mv |
71755985304 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8800103074322967 |
dc.contributor.referee1.fl_str_mv |
Euclides Gomes Parente Filho |
dc.contributor.referee1ID.fl_str_mv |
26338440300 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8405955314901291 |
dc.contributor.referee2.fl_str_mv |
AndrÃa Silvia Walter de Aguiar |
dc.contributor.referee2ID.fl_str_mv |
31761500325 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0341117842153741 |
dc.contributor.authorID.fl_str_mv |
75733463368 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1556885629243172 |
dc.contributor.author.fl_str_mv |
FÃbio Solon Tajra |
contributor_str_mv |
Edson Holanda Teixeira Euclides Gomes Parente Filho AndrÃa Silvia Walter de Aguiar |
dc.subject.por.fl_str_mv |
CÃrie DentÃria Lectinas Potencial BiotecnolÃgico |
topic |
CÃrie DentÃria Lectinas Potencial BiotecnolÃgico Dental Caries Lectins Biotechnological Potentialities BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Dental Caries Lectins Biotechnological Potentialities |
dc.subject.cnpq.fl_str_mv |
BIOQUIMICA |
dc.description.sponsorship.fl_txt_mv |
FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico |
dc.description.abstract.por.fl_txt_mv |
Dental Caries is an infectious disease naturally multi-factorial that begins in the childhood, able to spread its intensity and prevalence according to the mouth ambient conditions of each host. The risk of the patients to develop, in some time of their lives, carious lesions can be measured through a complex analysis using health determinant factors and other specific factors of the health-disease process. Since the dental caries forward diagnostic as well as the review of the auxiliary determinant factors of the dental caries danger evaluation, the treatment becomes simpler, less invasive and costing lower. It has been quite broad-casted lately the use of lectins to diagnose and to prevent some diseases. When dealing with dental caries disease, through many years this strategy has been explored still inceptively. Looking through this perspective, the goal of this trial was to investigate the biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry. It is about an explanatory-experimental study of quantitative approach through the health questionnaire and data statistics analysis. The research makes reference to some comparative analysis of salivary tests and the cariogenic activity in twelve year-old students and it searches to evaluate the biotechnological potentialities of the lectin from the red marine alga Hypnea musciformis (HML) in the diagnose and prevention of the dental caries. The concentration area of this trial was the macromolecules/ lectin biotechnological application. In the present trial were compared obtained results through the application of the conventional clinical exams to dental caries and salivary tests of dental caries activity pointed by the CARIOGRAM program in association with lab analysis using specific lectins. From this trial, it has been concluded that, about lectins as a biotechnological potential to prevention and diagnose of the dental caries, it has been noticed that there is recognition of resident glycoconjugate on pacifier saliva of high and low risk observed in immunofluorescence pattern. The results showed that the tested lectin has the capacity to intervene on the bacterial growth in a negative way, it is being distinguished statistically (p<0,01) in relation to control group of saline and BSA. However, inhibition studies of the microbial biofilms using the HML are being programmed to evaluate the using possibility of these protein as biotech input to be used for dental caries prevention. It could be said also that the obtained results by the use of HML in the antimicrobial activity were promising, showing that this lectin has presented antimicrobial activity even on very small amounts. As to the use of these protein in the biofilm formation and developing there was no characteristic pattern. A cÃrie à uma doenÃa infecciosa de natureza multifatorial que se inicia na infÃncia, podendo aumentar de intensidade e prevalÃncia de acordo com as condiÃÃes do ambiente bucal de cada hospedeiro. O risco do paciente em desenvolver, em algum perÃodo de seu ciclo de vida, lesÃes cariosas pode ser medido atravÃs de uma anÃlise complexa que utiliza os fatores determinantes da saÃde e outros fatores especÃficos do processo saÃde-doenÃa. A partir do diagnÃstico precoce da doenÃa cÃrie, assim como anÃlise dos fatores determinantes auxiliares da avaliaÃÃo do risco de cÃrie, o tratamento torna-se mais simples, menos invasivo e de menor custo. Tem sido bastante difundida ultimamente a utilizaÃÃo de lectinas para diagnÃstico e prevenÃÃo de algumas doenÃas. Em se tratando da doenÃa cÃrie, ao longo de muitos anos, essa estratÃgia tem sido explorada ainda de forma incipiente. Nesta perspectiva, o objetivo deste trabalho foi investigar as potencialidades biotecnolÃgicas da lectina de alga marinha da espÃcie de Hypnea musciformis (HML) aplicadas à odontologia. Trata-se de um estudo explicativo-experimental de abordagem quantitativa atravÃs de questionÃrio de saÃde e anÃlises estatÃsticas de dados. A pesquisa faz referÃncia à uma anÃlise comparativa de testes salivares e atividade cariogÃnica em escolares de 12 anos de idade e a busca avaliar as potencialidades biotecnolÃgicas da lectina de alga marinha da espÃcie de Hypnea musciformis (HML) no diagnÃstico e prevenÃÃo de cÃrie dentÃria. A Ãrea de concentraÃÃo deste estudo MacromolÃtulas / AplicaÃÃo BiotecnolÃgica de Lectinas. No presente estudo, foram comparados os resultados obtidos atravÃs da aplicaÃÃo de exame clÃnico convencional para cÃrie e testes salivares de atividade cariogÃnica apontados pelo programa CARIOGRAM em associaÃÃo com anÃlises laboratoriais com a utilizaÃÃo de lectinas especÃficas. A partir deste estudo, concluiu-se que, em se tratando do uso de lectinas como potencial biotecnolÃgico para a prevenÃÃo e diagnÃstico de cÃrie dentÃria, percebeu-se que hà reconhecimento de glicoconjugados presentes na saliva de pacientes de alto e baixo risco observados em padrÃo de fluorescÃncia. Os resultados demonstram que a lectina testada tem a capacidade de interferir no crescimento bacteriano de forma negativa, sendo estatisticamente significante (p<0,01) em relaÃÃo aos grupos controle de salina e BSA. Entretanto estudos de inibiÃÃo da formaÃÃo de biofilmes microbianos utilizando a HML estÃo sendo programados, para assim avaliar a possibilidade de utilizaÃÃo dessas proteÃnas como insumos biotecnolÃgicos a ser utilizados para prevenÃÃo da cÃrie dentÃria. Pode-se ainda afirmar que os resultados obtidos pelo uso de HML na atividade antibacteriana foram promissores, mostrando que esta lectina apresentou atividade antibacteriana mesmo em quantidades muito pequenas. Quanto ao uso destas proteÃnas na formaÃÃo e desenvolvimento de biofilmes, nÃo houve padrÃo caracterÃstico. |
description |
Dental Caries is an infectious disease naturally multi-factorial that begins in the childhood, able to spread its intensity and prevalence according to the mouth ambient conditions of each host. The risk of the patients to develop, in some time of their lives, carious lesions can be measured through a complex analysis using health determinant factors and other specific factors of the health-disease process. Since the dental caries forward diagnostic as well as the review of the auxiliary determinant factors of the dental caries danger evaluation, the treatment becomes simpler, less invasive and costing lower. It has been quite broad-casted lately the use of lectins to diagnose and to prevent some diseases. When dealing with dental caries disease, through many years this strategy has been explored still inceptively. Looking through this perspective, the goal of this trial was to investigate the biotechnological potential of the red marine alga Hypnea musciformis (HML) applied to Dentistry. It is about an explanatory-experimental study of quantitative approach through the health questionnaire and data statistics analysis. The research makes reference to some comparative analysis of salivary tests and the cariogenic activity in twelve year-old students and it searches to evaluate the biotechnological potentialities of the lectin from the red marine alga Hypnea musciformis (HML) in the diagnose and prevention of the dental caries. The concentration area of this trial was the macromolecules/ lectin biotechnological application. In the present trial were compared obtained results through the application of the conventional clinical exams to dental caries and salivary tests of dental caries activity pointed by the CARIOGRAMÂ program in association with lab analysis using specific lectins. From this trial, it has been concluded that, about lectins as a biotechnological potential to prevention and diagnose of the dental caries, it has been noticed that there is recognition of resident glycoconjugate on pacifier saliva of high and low risk observed in immunofluorescence pattern. The results showed that the tested lectin has the capacity to intervene on the bacterial growth in a negative way, it is being distinguished statistically (p<0,01) in relation to control group of saline and BSA. However, inhibition studies of the microbial biofilms using the HML are being programmed to evaluate the using possibility of these protein as biotech input to be used for dental caries prevention. It could be said also that the obtained results by the use of HML in the antimicrobial activity were promising, showing that this lectin has presented antimicrobial activity even on very small amounts. As to the use of these protein in the biofilm formation and developing there was no characteristic pattern. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.uri.fl_str_mv |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=5285 |
url |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=5285 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Biotecnologia (Campus da UFC em Sobral-CE) |
dc.publisher.initials.fl_str_mv |
UFC |
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BR |
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Universidade Federal do Cearà |
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reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Universidade Federal do Ceará |
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UFC |
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UFC |
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mail@mail.com |
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