Cultivo de Arthrospira platensis em meio orgÃnico: extraÃÃo de polissacarÃdeos sulfatados e sua administraÃÃo a camarÃes Penaeus vannamei

• Autor(a) principal: Renato Teixeira Moreira
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
• Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16577
• topic: Penaeus vannamei; Arthrospira platensis; polissacarÃdeos sulfatados; imunoestimulaÃÃo; Penaeus vannamei; Arthrospira platensis; sulphated polysaccharides; Immunoestimulation.; AQUICULTURA

Aquaculture takes up more and more space on the world stage, as a result of technological advances and increased demand for animal protein, consolidating itself as the fastest growing activity in agribusiness. On the other hand, there is the possibility of the development of illnesses over the cultivation, as well as impact on the environment, with the release of organic compounds in water, eutrophicating aquatic ecosystem. Given this scenario, microalgae Arthrospira platensis, as to appear as a strong ally of the producers, whether for absorption of organic compounds and treatment of effluents, is for the production of compounds that exhibit biological activity, promoting the welfare of farmed animals. Thus, the objective of this study was to produce. platensis cultivation sewage Nile tilapia (Oreochromis niloticus), extract and evaluate the immunomodulatory effect of sulfated polysaccharide (PS) in juvenile Penaeus vannamei. The PS were obtained from a hot aqueous extract and administered through immersion baths in juveniles of P. vannamei, at concentrations of 0, 60, 120 and 240 mg L-1. After soaking, the total count of hemocytes (THC) and checked the concentration of total proteins (CP) and activity of enzymes fenoloxidase (PO) and α2-macroglobulina. After the evaluation of the imunoparÃmetros, it was found that the concentration of 120 mg L-1 the best immunostimulating activity. This concentration was used to observe the reaction of immunological parameters when exposed to the weather (no exposure) 0, 45, 90, 135 and 180 minutes, as well as the antimicrobial activity against Vibrio alginolyticus. The microalgae showed a good development within the organic production system. Were extracted 19,85 g PS, with a yield of 8,5% of the biomass used. During the fractionation of PS were observed two fractions with 0,5 and 1,2 M majority of NaCl. After three hours of immersion in different concentrations of PS, the imunoparÃmetros showed an increase gradually as amounting to concentration of PS. Still, stimulation of the immune system of shrimp took place with 90 and 135 minutes of exposure to the PS. The fenoloxidase enzyme reached its maximum activity (72,33 Umin-1 mg-1) with 135 minutes of exposure, followed by an increase of the α2-macroglobulina enzyme activity with 180 minutes. The serum of animals collected with 90 and 135 minutes was more effective in inhibiting the development of V. alginolyticus, and needed only 1,51 Â 0,08 and 1,59 Â 0,02 mg mL-1 of proteins to fight it. The results obtained in other times did not differ from time zero. Thus, it is concluded that the PS of A. platensis were able to activate the immune system in shrimp shortly after 90 minutes of exposure, but also contributed to increase the antibacterial activity of the serum of animals.