Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Caatinga |
Texto Completo: | https://periodicos.ufersa.edu.br/caatinga/article/view/11415 |
Resumo: | Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops. |
id |
UFERSA-1_7addd3040c95cb5df40a53a1245cd2ac |
---|---|
oai_identifier_str |
oai:ojs.periodicos.ufersa.edu.br:article/11415 |
network_acronym_str |
UFERSA-1 |
network_name_str |
Revista Caatinga |
repository_id_str |
|
spelling |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plantsAvaliação da diversidade morfofisiológica e genotípica de bactérias endofíticas isoladas de arroz (Oryza sativa L.)Arroz. Promoção de crescimento de plantas. PCR 16S-23S rDNA. Enzimas extracelulares.ice. Plant growth promotion. PCR 16S-23S rDNA. Extracellular enzymes.Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops.O arroz apresenta altos custos de produção no Brasil devido ao elevado uso de agroquímicos. Algumas rizobactérias promotoras de crescimento de plantas (PGPR) podem ser utilizadas como alternativa aos fertilizantes e produtos fitossanitários. Este trabalho teve como objetivo caracterizar bactérias endofíticas obtidas de raízes de arroz. A caracterização dos isolados foi baseada na morfologia da colônia, resistência a antibióticos, uso de fontes de carbono, atividade enzimática (catalase, amilase, protease, celulase e lipase), solubilização de fosfato inorgânico e região intergênica do 16S-23S rDNA. Com base na morfologia, 68% dos isolados apresentaram crescimento rápido, 46% apresentaram produção abundante de muco e 77% apresentaram colônia viscosa. Todos os isolados foram resistentes ao ácido nalidíxico e 16% deles à estreptomicina. A maioria dos isolados (90%) utilizou monossacarídeos e dissacarídeos no ensaio da fonte de carbono. A maioria dos isolados (95%) foi positiva para catalase e 63,6% foram positivas para amilase, protease, lipase e celulase. Além disso, 59% foram capazes de solubilizar o fosfato. A média do índice enzimático para amilase, celulase e protease foi de 2,8, 3,5 e 1,7, respectivamente. A análise de similaridade revelou alta diversidade entre os isolados, com índice de similaridade de 70% (baseado nas características morfológicas) e 60% (baseado na análise da região intergênica 16S-23S rDNA). Com base nas características morfofisiológicas e genotípicas, três isolados promissores devem ser avaliados em estudos futuros em condições de campo, podendo gerar bioprodutos destinados a substituir insumos industrializados na cultura do arroz.Universidade Federal Rural do Semi-Árido2023-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1141510.1590/1983-21252023v36n405rcREVISTA CAATINGA; Vol. 36 No. 4 (2023); 775-784Revista Caatinga; v. 36 n. 4 (2023); 775-7841983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/11415/11319Copyright (c) 2023 Revista Caatingainfo:eu-repo/semantics/openAccessLeonardo-Silva, LucasSilva, Larissa Batista da Ferreira, Enderson Petrônio de BritoSantos, Karina Freire d'Eça NogueiraNaves, Plinio Lázaro FaleiroMartin-Didonet (In memoriam), Claudia Cristina Garcia2023-09-28T13:12:02Zoai:ojs.periodicos.ufersa.edu.br:article/11415Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:47:03.548189Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true |
dc.title.none.fl_str_mv |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants Avaliação da diversidade morfofisiológica e genotípica de bactérias endofíticas isoladas de arroz (Oryza sativa L.) |
title |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
spellingShingle |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants Leonardo-Silva, Lucas Arroz. Promoção de crescimento de plantas. PCR 16S-23S rDNA. Enzimas extracelulares. ice. Plant growth promotion. PCR 16S-23S rDNA. Extracellular enzymes. |
title_short |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
title_full |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
title_fullStr |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
title_full_unstemmed |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
title_sort |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants |
author |
Leonardo-Silva, Lucas |
author_facet |
Leonardo-Silva, Lucas Silva, Larissa Batista da Ferreira, Enderson Petrônio de Brito Santos, Karina Freire d'Eça Nogueira Naves, Plinio Lázaro Faleiro Martin-Didonet (In memoriam), Claudia Cristina Garcia |
author_role |
author |
author2 |
Silva, Larissa Batista da Ferreira, Enderson Petrônio de Brito Santos, Karina Freire d'Eça Nogueira Naves, Plinio Lázaro Faleiro Martin-Didonet (In memoriam), Claudia Cristina Garcia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Leonardo-Silva, Lucas Silva, Larissa Batista da Ferreira, Enderson Petrônio de Brito Santos, Karina Freire d'Eça Nogueira Naves, Plinio Lázaro Faleiro Martin-Didonet (In memoriam), Claudia Cristina Garcia |
dc.subject.por.fl_str_mv |
Arroz. Promoção de crescimento de plantas. PCR 16S-23S rDNA. Enzimas extracelulares. ice. Plant growth promotion. PCR 16S-23S rDNA. Extracellular enzymes. |
topic |
Arroz. Promoção de crescimento de plantas. PCR 16S-23S rDNA. Enzimas extracelulares. ice. Plant growth promotion. PCR 16S-23S rDNA. Extracellular enzymes. |
description |
Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11415 10.1590/1983-21252023v36n405rc |
url |
https://periodicos.ufersa.edu.br/caatinga/article/view/11415 |
identifier_str_mv |
10.1590/1983-21252023v36n405rc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufersa.edu.br/caatinga/article/view/11415/11319 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Revista Caatinga info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Revista Caatinga |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
dc.source.none.fl_str_mv |
REVISTA CAATINGA; Vol. 36 No. 4 (2023); 775-784 Revista Caatinga; v. 36 n. 4 (2023); 775-784 1983-2125 0100-316X reponame:Revista Caatinga instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Revista Caatinga |
collection |
Revista Caatinga |
repository.name.fl_str_mv |
Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
patricio@ufersa.edu.br|| caatinga@ufersa.edu.br |
_version_ |
1797674030058176512 |