Remoção do herbicida atrazina por fungos de decomposição branca

Detalhes bibliográficos
Autor(a) principal: Monteiro, Keyle Borges e Silva
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/3714
Resumo: Given the deterioration of water resources and the growing need to meet water quality standards, alternatives to remove micropollutants from aquatic environments have become necessary. Among micropollutants, we highlight atrazine (ATZ), from the class of triazine herbicides, classified as moderately toxic to humans and highly toxic to aquatic organisms, a potent endocrine disruptor and possibly a carcinogenic. Biotechnological processes have been utilized to minimize the toxic effects of pollutants on both humans and the environment, promoting the interaction between micro-organisms and their by-products, such as enzymes. This study aimed to experimentally describe the removal of ATZ from a final concentration of 100 mg.L-1 in liquid culture medium, utilizing rot-white fungi as a future contributor in the treatment of wastewater or public water supplies. Initially assessed the tolerance of Trametes villosa, Lentinus edodes, Pycnosporus sanguineus, Pleurotus ostreatus and Pleurotus sp., in PDA (Potato Dextrose Agar) culture with nutritional variations (PDA test standard, PDA 40% and PDA 0%) in the presence of atrazine at a concentration of 100 mg. L-1 and above an application value of 500 mg.L-1. Next conducted a test 2.2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium 0.02% (ABTS) to assess the production of ligninolytic enzymes. In these stages, the microorganisms showed both tolerance to atrazine toxicity and growth through each nutritional condition assessed, displaying decrease in growth rate at a concentration of ATZ500 mg.L-1.Morphology and mycelial growth were unsatisfactory at a low nutrient concentration (PDA 0%) when compared with PDA test standard and PDA 40%. Following a 24 hour culture period, enzymatic activity was detected through a colored halo around the microorganisms inoculant across all assessed conditions, except for Lentinus edodes and Trametes villosa, which showed activity after 96 hours, the latter only in PDA 0% culture in the presence and absence of herbicide, therefore selected for the ATZ degradation experiments in liquid culture medium. Pleurotus ostreatus and Pleurotus sp. in PDA 40% static condition yielded the highest removal concentration indexes.ATZ concentration decrease was obtained through enzymatic activity, especially that of laccase and manganese peroxidase, by means of adsorption in the biomass of the microorganisms. Atrazine concentration was determined by means of gas chromatography/high resolution mass spectrometry methods. Study results regarding Pleurotus sp., Pleurotus ostreatus and Pycnosporus sanguineus, under two distinct conditions (agitation and statics), showed their great potential in the bioremediation of aquatic environments contaminated by atrazine.
id UFG-2_12376f9c1a251ff0630478f4d573c470
oai_identifier_str oai:repositorio.bc.ufg.br:tede/3714
network_acronym_str UFG-2
network_name_str Repositório Institucional da UFG
repository_id_str
spelling Santiago, Mariângela Fonteshttp://lattes.cnpq.br/7143224488081563Santiago, Mariângela FontesAlmeida, Rogério de AraújoPonezi, Alexandre NunesMonteiro, Keyle Borges e Silva2014-12-01T20:04:46Z2013-08-12MONTEIRO, Keyle Borges e Silva. Remoção do herbicida atrazina por fungos de decomposição branca. 2013. 140 f. Dissertação (Mestrado em Engenharia do Meio Ambiente) - Universidade Federal de Goiás, Goiânia, 2013.http://repositorio.bc.ufg.br/tede/handle/tede/3714Given the deterioration of water resources and the growing need to meet water quality standards, alternatives to remove micropollutants from aquatic environments have become necessary. Among micropollutants, we highlight atrazine (ATZ), from the class of triazine herbicides, classified as moderately toxic to humans and highly toxic to aquatic organisms, a potent endocrine disruptor and possibly a carcinogenic. Biotechnological processes have been utilized to minimize the toxic effects of pollutants on both humans and the environment, promoting the interaction between micro-organisms and their by-products, such as enzymes. This study aimed to experimentally describe the removal of ATZ from a final concentration of 100 mg.L-1 in liquid culture medium, utilizing rot-white fungi as a future contributor in the treatment of wastewater or public water supplies. Initially assessed the tolerance of Trametes villosa, Lentinus edodes, Pycnosporus sanguineus, Pleurotus ostreatus and Pleurotus sp., in PDA (Potato Dextrose Agar) culture with nutritional variations (PDA test standard, PDA 40% and PDA 0%) in the presence of atrazine at a concentration of 100 mg. L-1 and above an application value of 500 mg.L-1. Next conducted a test 2.2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium 0.02% (ABTS) to assess the production of ligninolytic enzymes. In these stages, the microorganisms showed both tolerance to atrazine toxicity and growth through each nutritional condition assessed, displaying decrease in growth rate at a concentration of ATZ500 mg.L-1.Morphology and mycelial growth were unsatisfactory at a low nutrient concentration (PDA 0%) when compared with PDA test standard and PDA 40%. Following a 24 hour culture period, enzymatic activity was detected through a colored halo around the microorganisms inoculant across all assessed conditions, except for Lentinus edodes and Trametes villosa, which showed activity after 96 hours, the latter only in PDA 0% culture in the presence and absence of herbicide, therefore selected for the ATZ degradation experiments in liquid culture medium. Pleurotus ostreatus and Pleurotus sp. in PDA 40% static condition yielded the highest removal concentration indexes.ATZ concentration decrease was obtained through enzymatic activity, especially that of laccase and manganese peroxidase, by means of adsorption in the biomass of the microorganisms. Atrazine concentration was determined by means of gas chromatography/high resolution mass spectrometry methods. Study results regarding Pleurotus sp., Pleurotus ostreatus and Pycnosporus sanguineus, under two distinct conditions (agitation and statics), showed their great potential in the bioremediation of aquatic environments contaminated by atrazine.Diante da deterioração dos recursos hídricos e da crescente necessidade de atender aos padrões de qualidade da água, são necessárias alternativas para a remoção de micropoluentes de ambientes aquáticos. Dentre os micropoluentes, destaca-se a atrazina (ATZ), herbicida da classe dos triazínicos, classificado como medianamente tóxico para o ser humano e altamente tóxico para organismos aquáticos, além de ser um potente disruptor endócrino e possível agente carcinogênico. Processos biotecnológicos têm sido utilizados para minimizar os efeitos tóxicos dos poluentes para o ser humano e meio ambiente, cuja finalidade é interação entre os micro-organismos e os produtos produzidos por eles, como enzimas. O presente estudo descreve de forma experimental, a remoção da ATZ na concentração de 100 mg.L-1 em meio líquido, utilizando fungos de decomposição branca, com vistas a futuras aplicações em tratamento de águas residuárias ou de abastecimento. Inicialmente, foi testada a tolerância das espécies Trametes villosa, Lentinus edodes, Pycnosporus sanguineus, Pleurotus ostreatus e Pleurotus sp., em meio de cultura sólido contendo Batata, Dextrose e Agar (BDA) com variações nutricionais (BDA padrão, BDA 40% e BDA 0%) e atrazina nas concentrações de 100 mg.L-1 e 500 mg.L-1. Posteriormente, foi realizado ensaio com 2,2 –Azino-bis (3- etilbenzotiazolina-6 – ácido sulfônico) diamônia 0,02% (ABTS), para avaliação da produção de enzimas ligninolíticas. Nessas etapas, os micro-organismos apresentaram tolerância à toxicidade da atrazina, cresceram em cada condição nutricional avaliada com redução na velocidade de crescimento na concentração ATZ 500 mg.L-1. A morfologia e o desenvolvimento micelial não foram satisfatórios na baixa concentração de nutrientes (BDA 0%) quando comparados com os meios BDA padrão e BDA 40%. Após o período de 24 horas de cultivo, foi observada atividade enzimática com aparecimento do halo de coloração em torno do inóculo dos micro-organismos em todas as condições avaliadas, exceto as espécies Lentinus edodes e Trametes villosa que apresentaram atividade após 96 horas, e este último somente em meio de cultivo BDA 0%, na ausência e presença do herbicida, por isso não foram selecionados para os experimentos da degradação da ATZ em meios de cultura líquido. Os maiores índices de remoção foram obtidos com as espécies Pleurotus ostreatus e Pleurotus sp., no meio BD 40% em condição estática (87,5% e 87,3%), respectivamente. Os menores valores de remoção foram de 47,2% pela espécie Pycnosporus sanguineus e 53,8% pelo fungo Pleurotus sp. em BD 0%, também na condição estática. A redução da concentração da atrazina foi obtida pela atividade enzimática, com destaque para a lacase e manganês peroxidase, e pelo mecanismo de adsorção na biomassa dos micro-organimos. A concentração da atrazina foi determinada por meio do cromatógrafo gasoso com detector de massa. Os resultados dos estudos com as espécies Pleurotus sp., Pleurotus ostreatus e Pycnosporus sanguineus, sob duas condições (agitação e estática), apresentaram grande potencial para serem utilizados na biorremediação de meios líquidos contaminados com atrazina.Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-12-01T20:04:13Z No. of bitstreams: 2 Dissertação - Keyle Borges e Silva Monteiro - 2013.pdf: 4439277 bytes, checksum: f4c76f010f6dd19a6ec56bcf424fcc5f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2014-12-01T20:04:46Z (GMT) No. of bitstreams: 2 Dissertação - Keyle Borges e Silva Monteiro - 2013.pdf: 4439277 bytes, checksum: f4c76f010f6dd19a6ec56bcf424fcc5f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-12-01T20:04:46Z (GMT). No. of bitstreams: 2 Dissertação - Keyle Borges e Silva Monteiro - 2013.pdf: 4439277 bytes, checksum: f4c76f010f6dd19a6ec56bcf424fcc5f (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2013-08-12application/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/13122/Disserta%c3%a7%c3%a3o%20-%20Keyle%20Borges%20e%20Silva%20Monteiro%20-%202013.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Engenharia do Meio Ambiente (EEC)UFGBrasilEscola de Engenharia Civil - EEC (RG)http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBiorremediaçãoAtrazinaEnzimas ligninolíticasBioremediationAtrazineLigninolytic enzymesENGENHARIA SANITARIA::SANEAMENTO AMBIENTALRemoção do herbicida atrazina por fungos de decomposição brancaRemoval of herbicide atrazine through rot-white fungiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis10097020919728748476006006007240872516263155858018064043757118887reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGORIGINALDissertação - Keyle Borges e Silva Monteiro - 2013.pdfDissertação - Keyle Borges e Silva Monteiro - 2013.pdfapplication/pdf4439277http://repositorio.bc.ufg.br/tede/bitstreams/88556a73-427b-4112-9177-e04c97f20345/downloadf4c76f010f6dd19a6ec56bcf424fcc5fMD55LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://repositorio.bc.ufg.br/tede/bitstreams/a513bc8c-d4e9-49fd-9f1d-291e496c1dd9/downloadbd3efa91386c1718a7f26a329fdcb468MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.bc.ufg.br/tede/bitstreams/8c141430-055d-4b2d-bb06-94f5b8da5cba/download4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822302http://repositorio.bc.ufg.br/tede/bitstreams/cbf70582-6cd6-4ecc-9351-6ee88d5b452f/download1e0094e9d8adcf16b18effef4ce7ed83MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.bc.ufg.br/tede/bitstreams/592838d3-cb5b-4260-8bae-0913a97f3df7/download9da0b6dfac957114c6a7714714b86306MD54TEXTDissertação - Keyle Borges e Silva Monteiro - 2013.pdf.txtDissertação - Keyle Borges e Silva Monteiro - 2013.pdf.txtExtracted Texttext/plain212978http://repositorio.bc.ufg.br/tede/bitstreams/f0299db9-e2c3-429b-a51c-424ef57165a5/download23f552b160cd1b3ce546cf32bc4b2a7bMD56THUMBNAILDissertação - Keyle Borges e Silva Monteiro - 2013.pdf.jpgDissertação - Keyle Borges e Silva Monteiro - 2013.pdf.jpgGenerated Thumbnailimage/jpeg1943http://repositorio.bc.ufg.br/tede/bitstreams/a29697bb-eb5a-483c-8f29-3f24f4a60bbf/downloadcc73c4c239a4c332d642ba1e7c7a9fb2MD57tede/37142014-12-02 03:01:15.55http://creativecommons.org/licenses/by-nc-nd/4.0/Acesso Abertoopen.accessoai:repositorio.bc.ufg.br:tede/3714http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2014-12-02T05:01:15Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)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
dc.title.por.fl_str_mv Remoção do herbicida atrazina por fungos de decomposição branca
dc.title.alternative.eng.fl_str_mv Removal of herbicide atrazine through rot-white fungi
title Remoção do herbicida atrazina por fungos de decomposição branca
spellingShingle Remoção do herbicida atrazina por fungos de decomposição branca
Monteiro, Keyle Borges e Silva
Biorremediação
Atrazina
Enzimas ligninolíticas
Bioremediation
Atrazine
Ligninolytic enzymes
ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL
title_short Remoção do herbicida atrazina por fungos de decomposição branca
title_full Remoção do herbicida atrazina por fungos de decomposição branca
title_fullStr Remoção do herbicida atrazina por fungos de decomposição branca
title_full_unstemmed Remoção do herbicida atrazina por fungos de decomposição branca
title_sort Remoção do herbicida atrazina por fungos de decomposição branca
author Monteiro, Keyle Borges e Silva
author_facet Monteiro, Keyle Borges e Silva
author_role author
dc.contributor.advisor1.fl_str_mv Santiago, Mariângela Fontes
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7143224488081563
dc.contributor.referee1.fl_str_mv Santiago, Mariângela Fontes
dc.contributor.referee2.fl_str_mv Almeida, Rogério de Araújo
dc.contributor.referee3.fl_str_mv Ponezi, Alexandre Nunes
dc.contributor.author.fl_str_mv Monteiro, Keyle Borges e Silva
contributor_str_mv Santiago, Mariângela Fontes
Santiago, Mariângela Fontes
Almeida, Rogério de Araújo
Ponezi, Alexandre Nunes
dc.subject.por.fl_str_mv Biorremediação
Atrazina
Enzimas ligninolíticas
topic Biorremediação
Atrazina
Enzimas ligninolíticas
Bioremediation
Atrazine
Ligninolytic enzymes
ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL
dc.subject.eng.fl_str_mv Bioremediation
Atrazine
Ligninolytic enzymes
dc.subject.cnpq.fl_str_mv ENGENHARIA SANITARIA::SANEAMENTO AMBIENTAL
description Given the deterioration of water resources and the growing need to meet water quality standards, alternatives to remove micropollutants from aquatic environments have become necessary. Among micropollutants, we highlight atrazine (ATZ), from the class of triazine herbicides, classified as moderately toxic to humans and highly toxic to aquatic organisms, a potent endocrine disruptor and possibly a carcinogenic. Biotechnological processes have been utilized to minimize the toxic effects of pollutants on both humans and the environment, promoting the interaction between micro-organisms and their by-products, such as enzymes. This study aimed to experimentally describe the removal of ATZ from a final concentration of 100 mg.L-1 in liquid culture medium, utilizing rot-white fungi as a future contributor in the treatment of wastewater or public water supplies. Initially assessed the tolerance of Trametes villosa, Lentinus edodes, Pycnosporus sanguineus, Pleurotus ostreatus and Pleurotus sp., in PDA (Potato Dextrose Agar) culture with nutritional variations (PDA test standard, PDA 40% and PDA 0%) in the presence of atrazine at a concentration of 100 mg. L-1 and above an application value of 500 mg.L-1. Next conducted a test 2.2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium 0.02% (ABTS) to assess the production of ligninolytic enzymes. In these stages, the microorganisms showed both tolerance to atrazine toxicity and growth through each nutritional condition assessed, displaying decrease in growth rate at a concentration of ATZ500 mg.L-1.Morphology and mycelial growth were unsatisfactory at a low nutrient concentration (PDA 0%) when compared with PDA test standard and PDA 40%. Following a 24 hour culture period, enzymatic activity was detected through a colored halo around the microorganisms inoculant across all assessed conditions, except for Lentinus edodes and Trametes villosa, which showed activity after 96 hours, the latter only in PDA 0% culture in the presence and absence of herbicide, therefore selected for the ATZ degradation experiments in liquid culture medium. Pleurotus ostreatus and Pleurotus sp. in PDA 40% static condition yielded the highest removal concentration indexes.ATZ concentration decrease was obtained through enzymatic activity, especially that of laccase and manganese peroxidase, by means of adsorption in the biomass of the microorganisms. Atrazine concentration was determined by means of gas chromatography/high resolution mass spectrometry methods. Study results regarding Pleurotus sp., Pleurotus ostreatus and Pycnosporus sanguineus, under two distinct conditions (agitation and statics), showed their great potential in the bioremediation of aquatic environments contaminated by atrazine.
publishDate 2013
dc.date.issued.fl_str_mv 2013-08-12
dc.date.accessioned.fl_str_mv 2014-12-01T20:04:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTEIRO, Keyle Borges e Silva. Remoção do herbicida atrazina por fungos de decomposição branca. 2013. 140 f. Dissertação (Mestrado em Engenharia do Meio Ambiente) - Universidade Federal de Goiás, Goiânia, 2013.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/3714
identifier_str_mv MONTEIRO, Keyle Borges e Silva. Remoção do herbicida atrazina por fungos de decomposição branca. 2013. 140 f. Dissertação (Mestrado em Engenharia do Meio Ambiente) - Universidade Federal de Goiás, Goiânia, 2013.
url http://repositorio.bc.ufg.br/tede/handle/tede/3714
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 1009702091972874847
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv 724087251626315585
dc.relation.cnpq.fl_str_mv 8018064043757118887
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Engenharia do Meio Ambiente (EEC)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Escola de Engenharia Civil - EEC (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Repositório Institucional da UFG
collection Repositório Institucional da UFG
bitstream.url.fl_str_mv http://repositorio.bc.ufg.br/tede/bitstreams/88556a73-427b-4112-9177-e04c97f20345/download
http://repositorio.bc.ufg.br/tede/bitstreams/a513bc8c-d4e9-49fd-9f1d-291e496c1dd9/download
http://repositorio.bc.ufg.br/tede/bitstreams/8c141430-055d-4b2d-bb06-94f5b8da5cba/download
http://repositorio.bc.ufg.br/tede/bitstreams/cbf70582-6cd6-4ecc-9351-6ee88d5b452f/download
http://repositorio.bc.ufg.br/tede/bitstreams/592838d3-cb5b-4260-8bae-0913a97f3df7/download
http://repositorio.bc.ufg.br/tede/bitstreams/f0299db9-e2c3-429b-a51c-424ef57165a5/download
http://repositorio.bc.ufg.br/tede/bitstreams/a29697bb-eb5a-483c-8f29-3f24f4a60bbf/download
bitstream.checksum.fl_str_mv f4c76f010f6dd19a6ec56bcf424fcc5f
bd3efa91386c1718a7f26a329fdcb468
4afdbb8c545fd630ea7db775da747b2f
1e0094e9d8adcf16b18effef4ce7ed83
9da0b6dfac957114c6a7714714b86306
23f552b160cd1b3ce546cf32bc4b2a7b
cc73c4c239a4c332d642ba1e7c7a9fb2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
_version_ 1798044372271366144