Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga

• Autor(a) principal: Martins, Marcos Pereira
• Data de Publicação: 2022
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/12099
• topic: Cnidoscolus; Flavonoides glicosilados; Esteroides; Molecular networking; Global natural products social molecular networking; Cnidoscolus; Flavonoids glycosides; Steroids; Molecular networking; Global natural products social molecular networking; CIENCIAS EXATAS E DA TERRA::QUIMICA

The genus Cnidoscolus belongs to the Euphorbiaceae family, is popularly known as cansanção or favela, and is distributed in several countries in America. In Brazil, this genus is mainly present in the Caatinga phytogeographic domain and has several species widely used by traditional communities. However, there are few chemical and pharmacological studies on this genus and there are no works description involving the annotation of compounds by Molecular Networking. In order to expand the chemical knowledge about the species C. bahianus, C. urniger and C. vitifolius, Nuclear Magnetic Resonance (NMR) and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS), principal component analysis (PCA), analyzes were performed involving extracts from leaves and stems, and some metabolites were isolated by filter column chromatography. The antimicrobial activities of C. bahianus, C. urniger and C. vitifolius extracts against Escherichia coli and Staphylococcus aureus strains were evaluated to determine the Minimum Inhibitory Concentration (MIC). The PCA of the 1H NMR data of the extracts revealed that the chemical profile of the species is similar. The analysis of the metabolic classes of the compounds from Cnidoscolus species by HPLC-IES-MS/MS (positive and negative modes) with consequent construction of molecular networking of the studied species led to the dereplication of phenolic compounds with the annotation of 40 flavonoids (class of flavonols, flavanols, flavones and flavanones) and 4 hydroxycinnamic acids. The metabolites isolated by filter chromatography were characterized by NMR as belonging to the steroid class (sitosterol, stigmasterol, campesterol and fucosterol). The ethanolic extract of C. bahianus leaves showed moderate antimicrobial activity and the others showed no activity for the tested strains.