Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/12099 |
Resumo: | The genus Cnidoscolus belongs to the Euphorbiaceae family, is popularly known as cansanção or favela, and is distributed in several countries in America. In Brazil, this genus is mainly present in the Caatinga phytogeographic domain and has several species widely used by traditional communities. However, there are few chemical and pharmacological studies on this genus and there are no works description involving the annotation of compounds by Molecular Networking. In order to expand the chemical knowledge about the species C. bahianus, C. urniger and C. vitifolius, Nuclear Magnetic Resonance (NMR) and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS), principal component analysis (PCA), analyzes were performed involving extracts from leaves and stems, and some metabolites were isolated by filter column chromatography. The antimicrobial activities of C. bahianus, C. urniger and C. vitifolius extracts against Escherichia coli and Staphylococcus aureus strains were evaluated to determine the Minimum Inhibitory Concentration (MIC). The PCA of the 1H NMR data of the extracts revealed that the chemical profile of the species is similar. The analysis of the metabolic classes of the compounds from Cnidoscolus species by HPLC-IES-MS/MS (positive and negative modes) with consequent construction of molecular networking of the studied species led to the dereplication of phenolic compounds with the annotation of 40 flavonoids (class of flavonols, flavanols, flavones and flavanones) and 4 hydroxycinnamic acids. The metabolites isolated by filter chromatography were characterized by NMR as belonging to the steroid class (sitosterol, stigmasterol, campesterol and fucosterol). The ethanolic extract of C. bahianus leaves showed moderate antimicrobial activity and the others showed no activity for the tested strains. |
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Kato, Luciliahttp://lattes.cnpq.br/1362891438631386Kato, LuciliaLião, Luciano MoraisSeverino, Vanessa Gisele PasqualottoLemes, Geralda de FátimaPaiva, Joseilson Alves dehttp://lattes.cnpq.br/2175015895028692Martins, Marcos Pereira2022-06-06T12:01:48Z2022-06-06T12:01:48Z2022-04-06MARTINS, M. P. Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga. 2022. 160 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2022.http://repositorio.bc.ufg.br/tede/handle/tede/12099ark:/38995/0013000006b2fThe genus Cnidoscolus belongs to the Euphorbiaceae family, is popularly known as cansanção or favela, and is distributed in several countries in America. In Brazil, this genus is mainly present in the Caatinga phytogeographic domain and has several species widely used by traditional communities. However, there are few chemical and pharmacological studies on this genus and there are no works description involving the annotation of compounds by Molecular Networking. In order to expand the chemical knowledge about the species C. bahianus, C. urniger and C. vitifolius, Nuclear Magnetic Resonance (NMR) and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS), principal component analysis (PCA), analyzes were performed involving extracts from leaves and stems, and some metabolites were isolated by filter column chromatography. The antimicrobial activities of C. bahianus, C. urniger and C. vitifolius extracts against Escherichia coli and Staphylococcus aureus strains were evaluated to determine the Minimum Inhibitory Concentration (MIC). The PCA of the 1H NMR data of the extracts revealed that the chemical profile of the species is similar. The analysis of the metabolic classes of the compounds from Cnidoscolus species by HPLC-IES-MS/MS (positive and negative modes) with consequent construction of molecular networking of the studied species led to the dereplication of phenolic compounds with the annotation of 40 flavonoids (class of flavonols, flavanols, flavones and flavanones) and 4 hydroxycinnamic acids. The metabolites isolated by filter chromatography were characterized by NMR as belonging to the steroid class (sitosterol, stigmasterol, campesterol and fucosterol). The ethanolic extract of C. bahianus leaves showed moderate antimicrobial activity and the others showed no activity for the tested strains.O gênero Cnidoscolus, pertencente à família Euphorbiaceae, é conhecido popularmente como cansanção ou favela e está distribuído em diversos países da América. No Brasil, está presente principalmente no domínio fitogeográfico Caatinga e possui várias espécies amplamente utilizadas por comunidades tradicionais. No entanto, há poucos estudos químicos e farmacológicos sobre o gênero e não há a descrição de trabalhos envolvendo a anotação de compostos por Molecular Networking. Visando ampliar o conhecimento químico sobre as espécies C. bahianus, C. urniger e C. vitifolius foram realizadas análises, envolvendo os extratos das folhas e caules, de Ressonância Magnética Nuclear (RMN), de cromatografia líquida de alta eficiência acoplada à espectrometria de massas com ionização por eletrospray (CLAE-IESEM/EM), análise de componentes principais (ACP) e o isolamento de alguns metabólitos por cromatografia em coluna filtrante. A atividade microbiana dos extratos de C. bahianus, C. urniger e C. vitifolius frente às cepas Escherichia coli e Staphylococcus aureus foram avaliadas para a determinação da Concentração Inibitória Mínima (CIM). A ACP dos dados de RMN de hidrogênio dos extratos revelou que o perfil químico das espécies é semelhante. A análise das classes metabólicas presentes nas espécies de Cnidoscolus por CLAE-IES-EM/EM (modos positivo e negativo) com consequente construção de redes moleculares das espécies estudadas, levou à desreplicação de compostos fenólicos com a anotação de 40 flavonoides (da classe dos flavonois, flavanois, flavonas e flavanonas) e 4 ácidos hidroxicinâmicos. Os metabólitos isolados por cromatografia filtrante foram caracterizados por RMN como pertencentes à classe dos esteroides (sitosterol, estigmasterol, campesterol e fucosterol). O extrato etanólico das folhas de C. bahianus apresentou atividade antimicrobiana moderada e os demais não apresentaram atividade para as cepas testadas.Submitted by Leandro Machado (leandromachado@ufg.br) on 2022-06-02T18:32:21Z No. of bitstreams: 2 Tese - Marcos Pereira Martins - 2022.pdf: 6416198 bytes, checksum: abc40b0fe05eb935280fd2bc17f0aa1d (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2022-06-06T12:01:48Z (GMT) No. of bitstreams: 2 Tese - Marcos Pereira Martins - 2022.pdf: 6416198 bytes, checksum: abc40b0fe05eb935280fd2bc17f0aa1d (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5)Made available in DSpace on 2022-06-06T12:01:48Z (GMT). No. of bitstreams: 2 Tese - Marcos Pereira Martins - 2022.pdf: 6416198 bytes, checksum: abc40b0fe05eb935280fd2bc17f0aa1d (MD5) license_rdf: 805 bytes, checksum: 4460e5956bc1d1639be9ae6146a50347 (MD5) Previous issue date: 2022-04-06Fundação de Amparo à Pesquisa do Estado de GoiásporUniversidade Federal de GoiásPrograma de Pós-graduação em Química (IQ)UFGBrasilInstituto de Química - IQ (RG)Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCnidoscolusFlavonoides glicosiladosEsteroidesMolecular networkingGlobal natural products social molecular networkingCnidoscolusFlavonoids glycosidesSteroidsMolecular networkingGlobal natural products social molecular networkingCIENCIAS EXATAS E DA TERRA::QUIMICAContribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da CaatingaContribution to the metabolomic study of species of the genus Cnidoscolus (Euphorbiaceae) from the Cerrado and Caatinga regionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis83500500500500291903reponame:Repositório Institucional da UFGinstname:Universidade Federal de Goiás (UFG)instacron:UFGLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.bc.ufg.br/tede/bitstreams/acea9bb8-7daf-4f54-b19c-8278a81db68b/download8a4605be74aa9ea9d79846c1fba20a33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.bc.ufg.br/tede/bitstreams/e630f3e4-3375-4f61-ad81-6f6c21d89bb0/download4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTese - Marcos Pereira Martins - 2022.pdfTese - Marcos Pereira Martins - 2022.pdfapplication/pdf6416198http://repositorio.bc.ufg.br/tede/bitstreams/7190262e-9947-40d3-8e93-a9d05a1868f5/downloadabc40b0fe05eb935280fd2bc17f0aa1dMD53tede/120992022-06-06 09:01:48.482http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internationalopen.accessoai:repositorio.bc.ufg.br:tede/12099http://repositorio.bc.ufg.br/tedeRepositório InstitucionalPUBhttp://repositorio.bc.ufg.br/oai/requesttasesdissertacoes.bc@ufg.bropendoar:2022-06-06T12:01:48Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)falseTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |
dc.title.pt_BR.fl_str_mv |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
dc.title.alternative.eng.fl_str_mv |
Contribution to the metabolomic study of species of the genus Cnidoscolus (Euphorbiaceae) from the Cerrado and Caatinga regions |
title |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
spellingShingle |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga Martins, Marcos Pereira Cnidoscolus Flavonoides glicosilados Esteroides Molecular networking Global natural products social molecular networking Cnidoscolus Flavonoids glycosides Steroids Molecular networking Global natural products social molecular networking CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
title_full |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
title_fullStr |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
title_full_unstemmed |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
title_sort |
Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga |
author |
Martins, Marcos Pereira |
author_facet |
Martins, Marcos Pereira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Kato, Lucilia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1362891438631386 |
dc.contributor.referee1.fl_str_mv |
Kato, Lucilia |
dc.contributor.referee2.fl_str_mv |
Lião, Luciano Morais |
dc.contributor.referee3.fl_str_mv |
Severino, Vanessa Gisele Pasqualotto |
dc.contributor.referee4.fl_str_mv |
Lemes, Geralda de Fátima |
dc.contributor.referee5.fl_str_mv |
Paiva, Joseilson Alves de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2175015895028692 |
dc.contributor.author.fl_str_mv |
Martins, Marcos Pereira |
contributor_str_mv |
Kato, Lucilia Kato, Lucilia Lião, Luciano Morais Severino, Vanessa Gisele Pasqualotto Lemes, Geralda de Fátima Paiva, Joseilson Alves de |
dc.subject.por.fl_str_mv |
Cnidoscolus Flavonoides glicosilados Esteroides |
topic |
Cnidoscolus Flavonoides glicosilados Esteroides Molecular networking Global natural products social molecular networking Cnidoscolus Flavonoids glycosides Steroids Molecular networking Global natural products social molecular networking CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Molecular networking Global natural products social molecular networking Cnidoscolus Flavonoids glycosides Steroids Molecular networking Global natural products social molecular networking |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The genus Cnidoscolus belongs to the Euphorbiaceae family, is popularly known as cansanção or favela, and is distributed in several countries in America. In Brazil, this genus is mainly present in the Caatinga phytogeographic domain and has several species widely used by traditional communities. However, there are few chemical and pharmacological studies on this genus and there are no works description involving the annotation of compounds by Molecular Networking. In order to expand the chemical knowledge about the species C. bahianus, C. urniger and C. vitifolius, Nuclear Magnetic Resonance (NMR) and high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS/MS), principal component analysis (PCA), analyzes were performed involving extracts from leaves and stems, and some metabolites were isolated by filter column chromatography. The antimicrobial activities of C. bahianus, C. urniger and C. vitifolius extracts against Escherichia coli and Staphylococcus aureus strains were evaluated to determine the Minimum Inhibitory Concentration (MIC). The PCA of the 1H NMR data of the extracts revealed that the chemical profile of the species is similar. The analysis of the metabolic classes of the compounds from Cnidoscolus species by HPLC-IES-MS/MS (positive and negative modes) with consequent construction of molecular networking of the studied species led to the dereplication of phenolic compounds with the annotation of 40 flavonoids (class of flavonols, flavanols, flavones and flavanones) and 4 hydroxycinnamic acids. The metabolites isolated by filter chromatography were characterized by NMR as belonging to the steroid class (sitosterol, stigmasterol, campesterol and fucosterol). The ethanolic extract of C. bahianus leaves showed moderate antimicrobial activity and the others showed no activity for the tested strains. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-06T12:01:48Z |
dc.date.available.fl_str_mv |
2022-06-06T12:01:48Z |
dc.date.issued.fl_str_mv |
2022-04-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, M. P. Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga. 2022. 160 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2022. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/12099 |
dc.identifier.dark.fl_str_mv |
ark:/38995/0013000006b2f |
identifier_str_mv |
MARTINS, M. P. Contribuição ao estudo metabolômico de espécies do gênero Cnidoscolus (Euphorbiaceae) da região do Cerrado e da Caatinga. 2022. 160 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2022. ark:/38995/0013000006b2f |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/12099 |
dc.language.iso.fl_str_mv |
por |
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por |
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500 500 500 500 |
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29 |
dc.relation.cnpq.fl_str_mv |
190 |
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3 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Química (IQ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Instituto de Química - IQ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
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reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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