Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti

Detalhes bibliográficos
Autor(a) principal: Romano, Camila Aline
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFG
Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tede/6230
Resumo: Aedes aegypti is the major vector of dengue, chikungunya and zika fever. Currently, with the lack of specific therapeutic and prophylactic measures, the main way to prevent these arboviruses is vector control. This control is performed mainly with the application of insecticides, but continuous use of these substances favored the emergence of resistant populations, making it necessary to develop alternative strategies for vector control. An example is the survey of vegetable substances which may interfere with the development of the mosquito. In this sense, the Cerrado plants the may be a promising way. In this study, was investigated the insecticidal activity of Cashew Nut Shell Liquid (CNSL) produced by the species Anacardium humile on Aedes aegypti, the dengue vector, Chikungunya and Zika virus. Fruits of A. humile were collected and kept in greenhouse of forced ventilation at 40º Celsius during seven days for the extraction of CNSL. Were performed mortality tests of third instar larvae (L3), pupa and adult, besides the observation of the oviposition behavior of females and viability of eggs and observation of the residual effect of solution. The CNSL was diluted in decreasing gradual concentrations from 1000 to 3ppm, to obtain the Lethal Concentrations (LC). In oviposition the LC99 was used for larvae. The tests of residual effect and oviposition were given in two stages, a pilot test of 100ppm and a test with the LC99. Was obtained LC50 and LC90, respectively, 6.63 and 11.23ppm for larvae; 276 and 728.62ppm for pupae, not being observed mortality in adults. The residual effect of the solution was seven days in the pilot test and five days in the LC99. In the pilot test for oviposition was observed repellent effect of CNSL on females with a significant reduction of eggs number (p = 0.0001 and p=0.0348) and change in standards of stratification of the eggs on oviposition substrate. The larval hatching rate of the eggs exposed to moistened substrate with CNSL was significantly lower (p = 0.0102) when compared to the control. The results presented evidence the insecticidal activity of the CNSL of A. humile, indicating it as a promising product in the research for new botanical insecticides. This way, further studies should be conducted in order to verify possible toxicity of the liquid to other animals.
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spelling Silva, Ionizete Garcia dahttp://lattes.cnpq.br/5021551669347602Arruda, WalquíriaSantos, Adelair Helena dosSilva, Ionizete Garcia daSilva, Heloisa Helena Garcia daCavasin, Glaucia Mariahttp://lattes.cnpq.br/9426933645023752Romano, Camila Aline2016-09-19T11:15:53Z2016-04-07ROMANO, C. A. Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti. 2016. 49 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2016.http://repositorio.bc.ufg.br/tede/handle/tede/6230Aedes aegypti is the major vector of dengue, chikungunya and zika fever. Currently, with the lack of specific therapeutic and prophylactic measures, the main way to prevent these arboviruses is vector control. This control is performed mainly with the application of insecticides, but continuous use of these substances favored the emergence of resistant populations, making it necessary to develop alternative strategies for vector control. An example is the survey of vegetable substances which may interfere with the development of the mosquito. In this sense, the Cerrado plants the may be a promising way. In this study, was investigated the insecticidal activity of Cashew Nut Shell Liquid (CNSL) produced by the species Anacardium humile on Aedes aegypti, the dengue vector, Chikungunya and Zika virus. Fruits of A. humile were collected and kept in greenhouse of forced ventilation at 40º Celsius during seven days for the extraction of CNSL. Were performed mortality tests of third instar larvae (L3), pupa and adult, besides the observation of the oviposition behavior of females and viability of eggs and observation of the residual effect of solution. The CNSL was diluted in decreasing gradual concentrations from 1000 to 3ppm, to obtain the Lethal Concentrations (LC). In oviposition the LC99 was used for larvae. The tests of residual effect and oviposition were given in two stages, a pilot test of 100ppm and a test with the LC99. Was obtained LC50 and LC90, respectively, 6.63 and 11.23ppm for larvae; 276 and 728.62ppm for pupae, not being observed mortality in adults. The residual effect of the solution was seven days in the pilot test and five days in the LC99. In the pilot test for oviposition was observed repellent effect of CNSL on females with a significant reduction of eggs number (p = 0.0001 and p=0.0348) and change in standards of stratification of the eggs on oviposition substrate. The larval hatching rate of the eggs exposed to moistened substrate with CNSL was significantly lower (p = 0.0102) when compared to the control. The results presented evidence the insecticidal activity of the CNSL of A. humile, indicating it as a promising product in the research for new botanical insecticides. This way, further studies should be conducted in order to verify possible toxicity of the liquid to other animals.Aedes aegypti é o principal vetor do dengue e das febres chikungunya e zika. Até o momento, devido à inexistência de medidas terapêuticas e profiláticas específicas, a principal forma de prevenção dessas arboviroses é o controle do vetor. Esse controle é realizado principalmente com aplicação de inseticidas, porém o uso contínuo dessas substâncias favoreceu o aparecimento de populações resistentes, tornando necessário o desenvolvimento de estratégias alternativas para o controle vetorial. Um exemplo é a pesquisa de substâncias vegetais que possam interferir no desenvolvimento do mosquito. Nesse sentido, as plantas do Cerrado podem ser um caminho promissor. Este trabalho investigou a atividade inseticida do líquido da castanha de caju (LCC) extraído da espécie Anacardium humile, planta nativa do Cerrado. Frutos de A. humile foram coletados e mantidos em estufa de ventilação forçada a 40ºC por sete dias para a extração do LCC. Foram realizados testes de mortalidade de larvas de terceiro estádio (L3), pupa e adulto. Além disso, observou-se o comportamento de oviposição das fêmeas, viabilidade dos ovos e o efeito residual da solução sobre L3. O LCC foi diluído em concentrações gradativas decrescentes de 1000 a 3ppm, para obtenção das concentrações letais (CL). Na oviposição foi empregada a CL99 para larvas. Os testes de efeito residual e oviposição se deram em duas etapas, um teste-piloto a 100ppm e um teste com a CL99. Obtiveram-se as CL50 e CL90 respectivamente, 6,63 e 11,23ppm para larvas; 276 e 728,62ppm para pupas. Não foi observada a mortalidade em adultos. O efeito residual da solução foi de sete dias no teste piloto e cinco dias na CL99. No teste piloto para oviposição observou-se efeito repelente do LCC sobre as fêmeas havendo redução significativa do número de ovos (p=0,0001 e p=0,0348) e mudança nos padrões de estratificação dos ovos no substrato de oviposição. Contudo, a taxa de eclosão larval dos ovos expostos ao substrato umedecido com LCC foi significativamente menor (p=0,0102) quando comparada ao controle. Os resultados apresentados evidenciam a atividade inseticida do LCC de A. humile, sugerindo-o como produto promissor na pesquisa por novos inseticidas botânicos. 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dc.title.por.fl_str_mv Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
dc.title.alternative.eng.fl_str_mv Insecticidal activity of nut shell liquid anacardium humile (anacardiaceae) against aedes aegypti
title Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
spellingShingle Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
Romano, Camila Aline
Controle vetorial
Bioinseticida
Cerrado
Anacardium humile
Vector control
Bioinsecticide
Cerrado
Anacardium humile
CIENCIAS BIOLOGICAS
title_short Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
title_full Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
title_fullStr Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
title_full_unstemmed Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
title_sort Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti
author Romano, Camila Aline
author_facet Romano, Camila Aline
author_role author
dc.contributor.advisor1.fl_str_mv Silva, Ionizete Garcia da
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5021551669347602
dc.contributor.referee1.fl_str_mv Arruda, Walquíria
dc.contributor.referee2.fl_str_mv Santos, Adelair Helena dos
dc.contributor.referee3.fl_str_mv Silva, Ionizete Garcia da
dc.contributor.referee4.fl_str_mv Silva, Heloisa Helena Garcia da
dc.contributor.referee5.fl_str_mv Cavasin, Glaucia Maria
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9426933645023752
dc.contributor.author.fl_str_mv Romano, Camila Aline
contributor_str_mv Silva, Ionizete Garcia da
Arruda, Walquíria
Santos, Adelair Helena dos
Silva, Ionizete Garcia da
Silva, Heloisa Helena Garcia da
Cavasin, Glaucia Maria
dc.subject.por.fl_str_mv Controle vetorial
Bioinseticida
Cerrado
Anacardium humile
topic Controle vetorial
Bioinseticida
Cerrado
Anacardium humile
Vector control
Bioinsecticide
Cerrado
Anacardium humile
CIENCIAS BIOLOGICAS
dc.subject.eng.fl_str_mv Vector control
Bioinsecticide
Cerrado
Anacardium humile
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS
description Aedes aegypti is the major vector of dengue, chikungunya and zika fever. Currently, with the lack of specific therapeutic and prophylactic measures, the main way to prevent these arboviruses is vector control. This control is performed mainly with the application of insecticides, but continuous use of these substances favored the emergence of resistant populations, making it necessary to develop alternative strategies for vector control. An example is the survey of vegetable substances which may interfere with the development of the mosquito. In this sense, the Cerrado plants the may be a promising way. In this study, was investigated the insecticidal activity of Cashew Nut Shell Liquid (CNSL) produced by the species Anacardium humile on Aedes aegypti, the dengue vector, Chikungunya and Zika virus. Fruits of A. humile were collected and kept in greenhouse of forced ventilation at 40º Celsius during seven days for the extraction of CNSL. Were performed mortality tests of third instar larvae (L3), pupa and adult, besides the observation of the oviposition behavior of females and viability of eggs and observation of the residual effect of solution. The CNSL was diluted in decreasing gradual concentrations from 1000 to 3ppm, to obtain the Lethal Concentrations (LC). In oviposition the LC99 was used for larvae. The tests of residual effect and oviposition were given in two stages, a pilot test of 100ppm and a test with the LC99. Was obtained LC50 and LC90, respectively, 6.63 and 11.23ppm for larvae; 276 and 728.62ppm for pupae, not being observed mortality in adults. The residual effect of the solution was seven days in the pilot test and five days in the LC99. In the pilot test for oviposition was observed repellent effect of CNSL on females with a significant reduction of eggs number (p = 0.0001 and p=0.0348) and change in standards of stratification of the eggs on oviposition substrate. The larval hatching rate of the eggs exposed to moistened substrate with CNSL was significantly lower (p = 0.0102) when compared to the control. The results presented evidence the insecticidal activity of the CNSL of A. humile, indicating it as a promising product in the research for new botanical insecticides. This way, further studies should be conducted in order to verify possible toxicity of the liquid to other animals.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-09-19T11:15:53Z
dc.date.issued.fl_str_mv 2016-04-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMANO, C. A. Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti. 2016. 49 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2016.
dc.identifier.uri.fl_str_mv http://repositorio.bc.ufg.br/tede/handle/tede/6230
identifier_str_mv ROMANO, C. A. Atividade inseticida do líquido da castanha de anacardium humile (anacardiaceae) sobre aedes aegypti. 2016. 49 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2016.
url http://repositorio.bc.ufg.br/tede/handle/tede/6230
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language por
dc.relation.program.fl_str_mv 5228863982809406209
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv -7769011444564556288
dc.relation.cnpq.fl_str_mv -3439178843068202161
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dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
dc.publisher.program.fl_str_mv Programa de Pós-graduação em Biologia das Interações Parasito-Hospedeiro (IPTSP)
dc.publisher.initials.fl_str_mv UFG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFG
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institution UFG
reponame_str Repositório Institucional da UFG
collection Repositório Institucional da UFG
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http://repositorio.bc.ufg.br/tede/bitstreams/b956cca4-6a55-4052-97c4-c818e0dd905a/download
http://repositorio.bc.ufg.br/tede/bitstreams/bc3d60b5-945a-4fbf-9b49-775ca6e3eb63/download
bitstream.checksum.fl_str_mv bd3efa91386c1718a7f26a329fdcb468
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bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFG - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv tasesdissertacoes.bc@ufg.br
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