Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/8345 |
Resumo: | Two experiments were carried out to evaluate the effect of the addition of 0.02% protease enzyme (Ronozyme ProAct) on diets based on corn and soybean meal for broilers. In both experiments, the same treatments used: T1- control ration, T2- a value of the nutritional matrix proposed by the enzyme but without the additive, T3- twice the value proposed in T2 without enzyme, T4- T2 with enzyme, T5- T3 with enzyme. In the first experiment, to determine the performance and yield of carcass and cuts were housed 1200 broiler chicks of 1 day of age, distributed in a completely randomized design, totaling 5 treatments, with 8 replicates and 30 birds per box. In the second experiment for the determination of metabolizable coefficients and intestinal histomorphometry, the method of total collection of excreta and intestinal tract collection was used in 300 chicks housed in galvanized steel cages at 1 day of age, distributed in a completely randomized design , submitted to 5 treatments and 6 replicates of 10 birds each. In both experiments, data analysis was perform using analysis of variance (ANOVA). In the first experiment, when the data were statistically different at 10% probability, they were compared by the Scott Knott Test and in the second experiment, when the data were statistically different at 5%, they were compared by the Scott Knott Test. In the second experiment we also performed a multivariate analysis of the main components and estimated the Pearson correlations. Chickens fed the one valued with enzyme treatment obtained the same final weight and weight gain as the control until 35 days of age.The birds that received the control treatment presented higher carcass yield, and in the weight of the cuts, there were no statistical differences between the protease treatments. Diets with protease showed higher dry matter metabolizable coefficient and nitrogen metabolizable coefficient, demonstrating that enzyme supplementation was effective. There was higher villus height and villus ratio: ileus crypt in broilers fed diets supplemented with protease at 7 days of age, however, at 21 days protease inclusion was not superior to the control treatment. A strong positive and significant correlation was observe between metabolizable and intestinal histomorphometry variables. Therefore, recommended to use protease in broiler diets. |
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Stringhini, José Henriquehttp://lattes.cnpq.br/8505634095383289Café, Marcos Barcelloshttp://lattes.cnpq.br/9860968235125158Stringhini, José HenriqueCarvalho, Fabyola Barros deJardim Filho, Roberto de Moareshttp://lattes.cnpq.br/6576089991436166Gomides, Larissa Paula Silva2018-04-18T11:05:47Z2018-03-28GOMIDES, L. P. S. Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte. 2018. 68 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2018.http://repositorio.bc.ufg.br/tede/handle/tede/8345ark:/38995/001300000f5xwTwo experiments were carried out to evaluate the effect of the addition of 0.02% protease enzyme (Ronozyme ProAct) on diets based on corn and soybean meal for broilers. In both experiments, the same treatments used: T1- control ration, T2- a value of the nutritional matrix proposed by the enzyme but without the additive, T3- twice the value proposed in T2 without enzyme, T4- T2 with enzyme, T5- T3 with enzyme. In the first experiment, to determine the performance and yield of carcass and cuts were housed 1200 broiler chicks of 1 day of age, distributed in a completely randomized design, totaling 5 treatments, with 8 replicates and 30 birds per box. In the second experiment for the determination of metabolizable coefficients and intestinal histomorphometry, the method of total collection of excreta and intestinal tract collection was used in 300 chicks housed in galvanized steel cages at 1 day of age, distributed in a completely randomized design , submitted to 5 treatments and 6 replicates of 10 birds each. In both experiments, data analysis was perform using analysis of variance (ANOVA). In the first experiment, when the data were statistically different at 10% probability, they were compared by the Scott Knott Test and in the second experiment, when the data were statistically different at 5%, they were compared by the Scott Knott Test. In the second experiment we also performed a multivariate analysis of the main components and estimated the Pearson correlations. Chickens fed the one valued with enzyme treatment obtained the same final weight and weight gain as the control until 35 days of age.The birds that received the control treatment presented higher carcass yield, and in the weight of the cuts, there were no statistical differences between the protease treatments. Diets with protease showed higher dry matter metabolizable coefficient and nitrogen metabolizable coefficient, demonstrating that enzyme supplementation was effective. There was higher villus height and villus ratio: ileus crypt in broilers fed diets supplemented with protease at 7 days of age, however, at 21 days protease inclusion was not superior to the control treatment. A strong positive and significant correlation was observe between metabolizable and intestinal histomorphometry variables. Therefore, recommended to use protease in broiler diets.Foram realizados dois experimentos com o objetivo de avaliar o efeito da adição da enzima protease 0,02% (Ronozyme ProAct) em dietas valorizadas a base de milho e farelo de soja para frangos de corte. Em ambos os experimentos foram utilizados os mesmos tratamentos: T1- ração controle, T2- uma valorização da matriz nutricional proposta pela enzima mas sem o aditivo, T3- o dobro da valorização proposta em T2 sem enzima, T4- T2 com enzima, T5- T3 com enzima. No primeiro experimento, para a determinação do desempenho e rendimento de carcaça e cortes, foram alojados 1200 pintos de corte de 1 dia de idade, distribuídos em delineamento inteiramente casualizado, totalizando 5 tratamentos, com 8 repetições e 30 aves por box. No segundo experimento para a determinação dos coeficientes de metabolizabilidade e histomorfometria intestinal, foi utilizado o método de coleta total de excretas e coleta dos seguimentos intestinais em 300 pintos de corte alojados em gaiolas de aço galvanizado com 1 dia de idade, distribuídos em delineamento inteiramente casualizado, submetidos à 5 tratamentos e 6 repetições de 10 aves cada. Em ambos experimentos, a análise de dados foi realizada utilizando-se a análise de variância (ANOVA). No primeiro experimento, quando os dados foram estatisticamente diferentes a 10% de probabilidade, foram comparados pelo Teste de Scott Knott e no segundo experimento, quando os dados foram estatisticamente diferentes a 5% foram comparados pelo Teste de Scott Knott. No segundo experimento também foi realizada análise multivariada de componentes principais e estimadas as correlações de Pearson. Frangos alimentados com o tratamento de uma valorização e inclusão da protease obtiveram o mesmo peso final e ganho de peso do tratamento controle até os 35 dias de idade. As aves que receberam o tratamento controle apresentaram maior rendimento de carcaça, e no peso dos cortes, não houve diferenças estatísticas entre os tratamentos com protease. As dietas que continham protease apresentaram maior coeficiente de metabolizabilidade da matéria seca (CMMS) e coeficiente de metabolizabilidade do nitrogênio (CMN), demonstrando que a suplementação da enzima foi eficaz. Houve maior altura de vilo e relação vilo:cripta do íleo em frangos alimentados com dietas suplementadas com protease aos 7 dias de idade, entretanto, aos 21 dias a inclusão da protease não foi superior ao tratamento controle. Foi observado forte correlação positiva e significativa entre as variáveis de metabolizabilidade e histomorfometria intestinal. Portanto, recomenda-se a utilização da protease em dietas para frangos de corte.Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2018-04-17T11:02:13Z No. of bitstreams: 2 Dissertação - Larissa Paula Silva Gomides - 2018.pdf: 1989079 bytes, checksum: 3bf945430c1e7aa48082c34c2ae9d96c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-04-18T11:05:47Z (GMT) No. of bitstreams: 2 Dissertação - Larissa Paula Silva Gomides - 2018.pdf: 1989079 bytes, checksum: 3bf945430c1e7aa48082c34c2ae9d96c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-04-18T11:05:47Z (GMT). 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dc.title.eng.fl_str_mv |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
dc.title.alternative.eng.fl_str_mv |
Protease monocomponent of Bacillus licheniformis in broiler diets |
title |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
spellingShingle |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte Gomides, Larissa Paula Silva Digestibilidade Desempenho Enzima exógena Nutrição Digestibility Exogenous enzyme Nutrition Performance ZOOTECNIA::PRODUCAO ANIMAL |
title_short |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
title_full |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
title_fullStr |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
title_full_unstemmed |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
title_sort |
Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte |
author |
Gomides, Larissa Paula Silva |
author_facet |
Gomides, Larissa Paula Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Stringhini, José Henrique |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8505634095383289 |
dc.contributor.advisor-co1.fl_str_mv |
Café, Marcos Barcellos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9860968235125158 |
dc.contributor.referee1.fl_str_mv |
Stringhini, José Henrique |
dc.contributor.referee2.fl_str_mv |
Carvalho, Fabyola Barros de |
dc.contributor.referee3.fl_str_mv |
Jardim Filho, Roberto de Moares |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6576089991436166 |
dc.contributor.author.fl_str_mv |
Gomides, Larissa Paula Silva |
contributor_str_mv |
Stringhini, José Henrique Café, Marcos Barcellos Stringhini, José Henrique Carvalho, Fabyola Barros de Jardim Filho, Roberto de Moares |
dc.subject.por.fl_str_mv |
Digestibilidade Desempenho Enzima exógena Nutrição |
topic |
Digestibilidade Desempenho Enzima exógena Nutrição Digestibility Exogenous enzyme Nutrition Performance ZOOTECNIA::PRODUCAO ANIMAL |
dc.subject.eng.fl_str_mv |
Digestibility Exogenous enzyme Nutrition Performance |
dc.subject.cnpq.fl_str_mv |
ZOOTECNIA::PRODUCAO ANIMAL |
description |
Two experiments were carried out to evaluate the effect of the addition of 0.02% protease enzyme (Ronozyme ProAct) on diets based on corn and soybean meal for broilers. In both experiments, the same treatments used: T1- control ration, T2- a value of the nutritional matrix proposed by the enzyme but without the additive, T3- twice the value proposed in T2 without enzyme, T4- T2 with enzyme, T5- T3 with enzyme. In the first experiment, to determine the performance and yield of carcass and cuts were housed 1200 broiler chicks of 1 day of age, distributed in a completely randomized design, totaling 5 treatments, with 8 replicates and 30 birds per box. In the second experiment for the determination of metabolizable coefficients and intestinal histomorphometry, the method of total collection of excreta and intestinal tract collection was used in 300 chicks housed in galvanized steel cages at 1 day of age, distributed in a completely randomized design , submitted to 5 treatments and 6 replicates of 10 birds each. In both experiments, data analysis was perform using analysis of variance (ANOVA). In the first experiment, when the data were statistically different at 10% probability, they were compared by the Scott Knott Test and in the second experiment, when the data were statistically different at 5%, they were compared by the Scott Knott Test. In the second experiment we also performed a multivariate analysis of the main components and estimated the Pearson correlations. Chickens fed the one valued with enzyme treatment obtained the same final weight and weight gain as the control until 35 days of age.The birds that received the control treatment presented higher carcass yield, and in the weight of the cuts, there were no statistical differences between the protease treatments. Diets with protease showed higher dry matter metabolizable coefficient and nitrogen metabolizable coefficient, demonstrating that enzyme supplementation was effective. There was higher villus height and villus ratio: ileus crypt in broilers fed diets supplemented with protease at 7 days of age, however, at 21 days protease inclusion was not superior to the control treatment. A strong positive and significant correlation was observe between metabolizable and intestinal histomorphometry variables. Therefore, recommended to use protease in broiler diets. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-18T11:05:47Z |
dc.date.issued.fl_str_mv |
2018-03-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOMIDES, L. P. S. Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte. 2018. 68 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2018. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/8345 |
dc.identifier.dark.fl_str_mv |
ark:/38995/001300000f5xw |
identifier_str_mv |
GOMIDES, L. P. S. Protease monocomponente de Bacillus liqueniformis nas dietas de frangos de corte. 2018. 68 f. Dissertação (Mestrado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2018. ark:/38995/001300000f5xw |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/8345 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
-8695879823182088786 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 |
dc.relation.department.fl_str_mv |
-6217552114249094582 |
dc.relation.cnpq.fl_str_mv |
-7513779660372594992 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.publisher.program.fl_str_mv |
Programa de Pós-graduação em Zootecnia (EVZ) |
dc.publisher.initials.fl_str_mv |
UFG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Veterinária e Zootecnia - EVZ (RG) |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFG instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
reponame_str |
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bitstream.checksum.fl_str_mv |
bd3efa91386c1718a7f26a329fdcb468 4afdbb8c545fd630ea7db775da747b2f d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e 3bf945430c1e7aa48082c34c2ae9d96c |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFG - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
tasesdissertacoes.bc@ufg.br |
_version_ |
1811721534028382208 |