Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFG |
Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/3066 |
Resumo: | Ruta graveolens has been successfully applied for many human diseases treatment and promises a well succeed alternative for plant diseases control because it has also phytoalexins in its composition. The aim of this study was to standardise the R. graveolens liquid extract and evaluate its potential for controlling rice (Oryza sativa) leaf blast (Magnaporthe oryzae). The drug has been characterized, the liquid extract obtained and the methodology for quantifying the standards components, furanocoumarins psoralen and bergapten, validated by high performance liquide chromatography. The components of essential oil obtained from standardized liquid extract were characterized by gas chromatography-mass spectrometry. In a completely randomized design, conducted in artificial hydrophobic surface with three replications and eleven treatments composed of M. oryzae conidial suspension (105 con.ml-1) mixed with R. graveolens standardized extract (0.01 to 0.10 g.mL-1), or furocoumarins psoralen (0.18 to 1.82 μg. mL-1), or bergapten (0.29 to 2.91 μg. mL-1), or water (control). It was evaluated the inhibition of conidial germination and appressorium formation and the median lethal dose (LD50). A second assay, in a completely randomized design in three replications was conducted in greenhouse conditions. It was composed of 21 days old rice plants, which were sprayed with a mixture containing M. oryzae conidial suspension (3x105 con.ml-1) and R. graveolens extract, without dilution, or furocoumarins psoralen (18.26 μg. mL-1), or bergapten (29.14 μg. mL-1), or water (control). Nine days after inoculation, leaf blast severity was scored with a diagrammatic scale, the data were statistically analyzed and means compared. The standardized plant extract inhibited M. oryzae conidial germination (LD50=0.237mg) and appressorium formation (LD50=0.121 mg) up to 100% and reduced 80.84% of leaf blast. By fluorescence microscopy it was possible to observe that standardized plant extract did not damage M. oryzae cell wall and plasma membrane, indicating another type of interaction to inhibit conidia development. Isolated standards furanocoumarins psoralen and bergapten did not inhibit conidial germination and appressorium formation and reduce leaf blast severity proportionally, suggesting that synergistic interactions between extract and essential oil components were responsible for the success of R. graveolens in suppressing rice disease, making it an alternative to compose rice blast management. |
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Conceição, Edemilson Cardoso dahttp://lattes.cnpq.br/7193007113950510Filippi, Marta Cristina Corsi dehttp://lattes.cnpq.br/0029536556461484Bresolin, Tania Mari Belléhttp://lattes.cnpq.br/2248201275641475Fonseca, Maria Jose Vieirahttp://lattes.cnpq.br/9252516331552117http://lattes.cnpq.br/0465499320826828Reis, Karinna Bannach2014-09-15T20:06:45Z2013-12-18REIS, Karinna Bannach. Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz. 2013.146 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2013.http://repositorio.bc.ufg.br/tede/handle/tede/3066Ruta graveolens has been successfully applied for many human diseases treatment and promises a well succeed alternative for plant diseases control because it has also phytoalexins in its composition. The aim of this study was to standardise the R. graveolens liquid extract and evaluate its potential for controlling rice (Oryza sativa) leaf blast (Magnaporthe oryzae). The drug has been characterized, the liquid extract obtained and the methodology for quantifying the standards components, furanocoumarins psoralen and bergapten, validated by high performance liquide chromatography. The components of essential oil obtained from standardized liquid extract were characterized by gas chromatography-mass spectrometry. In a completely randomized design, conducted in artificial hydrophobic surface with three replications and eleven treatments composed of M. oryzae conidial suspension (105 con.ml-1) mixed with R. graveolens standardized extract (0.01 to 0.10 g.mL-1), or furocoumarins psoralen (0.18 to 1.82 μg. mL-1), or bergapten (0.29 to 2.91 μg. mL-1), or water (control). It was evaluated the inhibition of conidial germination and appressorium formation and the median lethal dose (LD50). A second assay, in a completely randomized design in three replications was conducted in greenhouse conditions. It was composed of 21 days old rice plants, which were sprayed with a mixture containing M. oryzae conidial suspension (3x105 con.ml-1) and R. graveolens extract, without dilution, or furocoumarins psoralen (18.26 μg. mL-1), or bergapten (29.14 μg. mL-1), or water (control). Nine days after inoculation, leaf blast severity was scored with a diagrammatic scale, the data were statistically analyzed and means compared. The standardized plant extract inhibited M. oryzae conidial germination (LD50=0.237mg) and appressorium formation (LD50=0.121 mg) up to 100% and reduced 80.84% of leaf blast. By fluorescence microscopy it was possible to observe that standardized plant extract did not damage M. oryzae cell wall and plasma membrane, indicating another type of interaction to inhibit conidia development. Isolated standards furanocoumarins psoralen and bergapten did not inhibit conidial germination and appressorium formation and reduce leaf blast severity proportionally, suggesting that synergistic interactions between extract and essential oil components were responsible for the success of R. graveolens in suppressing rice disease, making it an alternative to compose rice blast management.Ruta graveolens é utilizada com sucesso no tratamento de diversas patologias humanas e possui potencial para o controle alternativo de fitopatógenos, pois também apresenta fitoalexinas em sua composição. O objetivo deste estudo foi padronizar o extrato vegetal líquido de R. graveolens e avaliar seu potencial para o controle da brusone foliar. O material vegetal foi caracterizado, o extrato líquido obtido e a metodologia para a quantificação dos padrões psoraleno e bergapteno foi validada por cromatografia líquida de alta eficiência. Os componentes do óleo essencial obtidos a partir do extrato líquido padronizado foram caracterizados por cromatografia gasosa acoplada à espectrometria de massas. No ensaio conduzido em superfície hidrofóbica artificial, com três repetições e onze tratamentos, 10 μL da suspensão de conídios de Magnaporthe oryzae (105 con/mL) foram misturadas com o extrato vegetal padronizado (0,01 a 0,10 g/mL), ou as furanocumarinas psoraleno (0,18 a 1,82 μg/mL) e bergapteno (0,29 a 2,91 μg/mL), ou água (controle). Avaliou-se a inibição da germinação conidial e da formação dos apressórios determinando-se a dose letal capaz de inibir 50% dos indivíduos (DL50). No experimento conduzido em casa de vegetação e com três repetições, plantas de arroz com 21 dias após plantio foram pulverizadas com uma suspensão de conídios de M. oryzae (3x105 com/mL) misturada com extrato vegetal padronizado sem diluição, ou psoraleno (18,26 μg/mL), ou bergapteno (29,14 μg/mL), ou água (controle). Após nove dias da inoculação, avaliou-se a severidade de brusone foliar com uma escala de 10 graus, os dados foram analisados estatisticamente e as médias comparadas. O extrato vegetal padronizado inibiu a formação de tubo germinativo (DL50= 0,237 mg) e a formação de apressório (DL50= 0,121 mg) de M. oryzae em até 100%, e reduziu a severidade de brusone nas folhas em 80,84%. Através de microscopia de fluorescência, não foi observada ação do extrato padronizado em membrana plasmática e parede celular de M. oryzae, o que indica outro tipo de interação para inibir o desenvolvimento do conídio. Os padrões psoraleno e bergapteno não inibiram proporcionalmente a germinação e a formação de apressório, como também não reduziram a severidade de brusone nas folhas em sua forma isolada, o que sugere que interações sinérgicas entre os diversos componentes do extrato e do óleo essencial de R. graveolens foram responsáveis pelo sucesso do extrato padronizado em suprimir a brusone foliar, tornando-o uma alternativa para compor o manejo de brusone em arroz.Submitted by Jaqueline Silva (jtas29@gmail.com) on 2014-09-15T20:06:22Z No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação Karinna Bannach Reis.pdf: 3955087 bytes, checksum: fbfd1023491588a9a5495cd281d72a41 (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-09-15T20:06:45Z (GMT) No. of bitstreams: 2 license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Dissertação Karinna Bannach Reis.pdf: 3955087 bytes, checksum: fbfd1023491588a9a5495cd281d72a41 (MD5)Made available in DSpace on 2014-09-15T20:06:45Z (GMT). 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dc.title.por.fl_str_mv |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
title |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
spellingShingle |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz Reis, Karinna Bannach Padronização Furanocumarina Psoraleno Bergapteno Óleo essencial Fitopatógeno Magnaporthe oryzae Standardization Furanocoumarin Psoralen Bergapten Essential oil Plant pathogen FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
title_short |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
title_full |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
title_fullStr |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
title_full_unstemmed |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
title_sort |
Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz |
author |
Reis, Karinna Bannach |
author_facet |
Reis, Karinna Bannach |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Conceição, Edemilson Cardoso da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7193007113950510 |
dc.contributor.advisor-co1.fl_str_mv |
Filippi, Marta Cristina Corsi de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0029536556461484 |
dc.contributor.referee1.fl_str_mv |
Bresolin, Tania Mari Bellé |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2248201275641475 |
dc.contributor.referee2.fl_str_mv |
Fonseca, Maria Jose Vieira |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9252516331552117 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0465499320826828 |
dc.contributor.author.fl_str_mv |
Reis, Karinna Bannach |
contributor_str_mv |
Conceição, Edemilson Cardoso da Filippi, Marta Cristina Corsi de Bresolin, Tania Mari Bellé Fonseca, Maria Jose Vieira |
dc.subject.por.fl_str_mv |
Padronização Furanocumarina Psoraleno Bergapteno Óleo essencial Fitopatógeno Magnaporthe oryzae |
topic |
Padronização Furanocumarina Psoraleno Bergapteno Óleo essencial Fitopatógeno Magnaporthe oryzae Standardization Furanocoumarin Psoralen Bergapten Essential oil Plant pathogen FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
dc.subject.eng.fl_str_mv |
Standardization Furanocoumarin Psoralen Bergapten Essential oil Plant pathogen |
dc.subject.cnpq.fl_str_mv |
FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS |
description |
Ruta graveolens has been successfully applied for many human diseases treatment and promises a well succeed alternative for plant diseases control because it has also phytoalexins in its composition. The aim of this study was to standardise the R. graveolens liquid extract and evaluate its potential for controlling rice (Oryza sativa) leaf blast (Magnaporthe oryzae). The drug has been characterized, the liquid extract obtained and the methodology for quantifying the standards components, furanocoumarins psoralen and bergapten, validated by high performance liquide chromatography. The components of essential oil obtained from standardized liquid extract were characterized by gas chromatography-mass spectrometry. In a completely randomized design, conducted in artificial hydrophobic surface with three replications and eleven treatments composed of M. oryzae conidial suspension (105 con.ml-1) mixed with R. graveolens standardized extract (0.01 to 0.10 g.mL-1), or furocoumarins psoralen (0.18 to 1.82 μg. mL-1), or bergapten (0.29 to 2.91 μg. mL-1), or water (control). It was evaluated the inhibition of conidial germination and appressorium formation and the median lethal dose (LD50). A second assay, in a completely randomized design in three replications was conducted in greenhouse conditions. It was composed of 21 days old rice plants, which were sprayed with a mixture containing M. oryzae conidial suspension (3x105 con.ml-1) and R. graveolens extract, without dilution, or furocoumarins psoralen (18.26 μg. mL-1), or bergapten (29.14 μg. mL-1), or water (control). Nine days after inoculation, leaf blast severity was scored with a diagrammatic scale, the data were statistically analyzed and means compared. The standardized plant extract inhibited M. oryzae conidial germination (LD50=0.237mg) and appressorium formation (LD50=0.121 mg) up to 100% and reduced 80.84% of leaf blast. By fluorescence microscopy it was possible to observe that standardized plant extract did not damage M. oryzae cell wall and plasma membrane, indicating another type of interaction to inhibit conidia development. Isolated standards furanocoumarins psoralen and bergapten did not inhibit conidial germination and appressorium formation and reduce leaf blast severity proportionally, suggesting that synergistic interactions between extract and essential oil components were responsible for the success of R. graveolens in suppressing rice disease, making it an alternative to compose rice blast management. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-18 |
dc.date.accessioned.fl_str_mv |
2014-09-15T20:06:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
REIS, Karinna Bannach. Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz. 2013.146 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/3066 |
identifier_str_mv |
REIS, Karinna Bannach. Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz. 2013.146 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2013. |
url |
http://repositorio.bc.ufg.br/tede/handle/tede/3066 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
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