Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni
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| Data de Publicação: | 2014 |
| Tipo de documento: | Tese |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFG |
| dARK ID: | ark:/38995/001300000153c |
| Texto Completo: | http://repositorio.bc.ufg.br/tede/handle/tede/3111 |
Resumo: | Biomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed that the calcium concentration in the hemolymph did not present significant difference (p>0.05) with regard to control as well as to the concentrations assessed. The glucose concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations increased; propionate in turn had its concentration decreased set against control in exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease. The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible to conclude that snails exposed to different concentrations of CaCO3 had their major metabolic alterations occurring from the 14th day of exposure. |
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Bezerra, José Clecildo Barretohttp://lattes.cnpq.br/9491755585617846Castro, Ana Maria deMello-Silva, Clélia Christina Corrê deBezerra, José Clecildo BarretoCastro, Ana maria deMello-Silva, Clélia Christina Corrêa deVinaud, Mariana ClareBarcelos, Rejane da Silva Senahttp://lattes.cnpq.br/3800305083164308Silva, Luciana Damacena2014-09-19T21:28:29Z2014-03-26Silva, Luciana Damacena. Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni. 2014. 90 f. Tese (Doutorado em Medicina Tropical e Saúde Pública) - Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) - Universidade Federal de Goiás, Goiânia, 2014.http://repositorio.bc.ufg.br/tede/handle/tede/3111ark:/38995/001300000153cBiomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed that the calcium concentration in the hemolymph did not present significant difference (p>0.05) with regard to control as well as to the concentrations assessed. The glucose concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations increased; propionate in turn had its concentration decreased set against control in exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease. The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible to conclude that snails exposed to different concentrations of CaCO3 had their major metabolic alterations occurring from the 14th day of exposure.Moluscos do gênero Biomphalaria são conhecidos pelo seu papel como hospedeiro intermediário do parasito Schistosoma mansoni, causador da doença esquistossomose mansônica. O aumento da população desses moluscos, consequentemente sua distribuição geográfica está condicionada a presença de cálcio no mbiente, também considerado um elemento essencial no metabolismo energético do molusco. Este estudo teve por objetivo avaliar a influência da exposição às diferentes concentrações de CaCO3 (20, 40, 60, 80 e 100 mg/L), em diferentes intervalos de tempo (1, 14, 21 e 30 dias) sobre às concentrações de glicose, cálcio, proteínas totais, ureia, ácido úrico e creatinina na hemolinfa de B. glabrata, ambas as substâncias foram dosadas através de kits específicos e lidas em espectrofotômetro em 545 nm. Através da técnica de cromatografia líquida de alta eficiência (CLAE), foi quantificado a presença de ácidos orgânicos: piruvato, oxaloacetato, citrato, succinato, fumarato, propionato, acetoacetato e β-hidroxibutirato na hemolinfa dos moluscos expostos ao CaCO3. Os resultados demonstraram que a concentração de cálcio na hemolinfa não apresentou diferença significativa (p>0,05) em relação ao controle, bem como, entre as concentrações testadas. A concentração de glicose diminuiu (p<0,05) nas exposições a 20mg e 40mg e aumentou nas exposições a 80mg e 100mg de CaCO3 em relação ao controle. Os ácidos orgânicos piruvato, oxaloacetato, citrato, succinato, fumarato e lactato tiveram suas concentrações aumentadas e propionato diminuída comparada ao controle na exposição ao CaCO3. A concentração de acetoacetato diminuiu e a de β-hidroxibutirato aumentou na exposição às concentrações testadas. Houve diminuição na concentração de proteínas totais e de ureia. A concentração de ácido úrico aumentou na exposição a 20mg de CaCO3 e, a concentração de creatinina aumentou nas exposições à 40mg e 100mg de CaCO3. Concluiu-se que moluscos expostos às diferentes concentrações de CaCO3, principalmente a partir de 14 dias, alteram o metabolismo como acontece em situações de estresse fisiológico, com a finalidade de regular o sistema redox do molusco.Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2014-09-19T20:22:37Z No. of bitstreams: 2 TESE LUCIANA DAMACENA SILVA (PDF).pdf: 994771 bytes, checksum: 9ea236448331d81f6039a107c7c58960 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2014-09-19T21:28:29Z (GMT) No. of bitstreams: 2 TESE LUCIANA DAMACENA SILVA (PDF).pdf: 994771 bytes, checksum: 9ea236448331d81f6039a107c7c58960 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)Made available in DSpace on 2014-09-19T21:28:29Z (GMT). No. of bitstreams: 2 TESE LUCIANA DAMACENA SILVA (PDF).pdf: 994771 bytes, checksum: 9ea236448331d81f6039a107c7c58960 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-03-26application/pdfhttp://repositorio.bc.ufg.br/tede/retrieve/8053/TESE%20LUCIANA%20DAMACENA%20SILVA%20%28PDF%29.pdf.jpgporUniversidade Federal de GoiásPrograma de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP)UFGBrasilFaculdade de Medicina - FM (RG)ABÍLIO, F.J.P.; RUFFO, T.L.M.; SOUZA, A.H.F.F.; FLORENTIN, H.S.; JÚNIOR, E.T.O.; MEIRELES, B.N.; SANTANA, A.C.D. Macroinvertebrados bentônicos como bioindicadores de qualidade ambiental de corpos aquáticos da caatinga Oecologia Brasiliensis, v. 11, n. 3, p. 397-409. 2007. ALLEN, P.J.; D’ANCI, K.E.; KANAREK, R.B. Creatine, brain functioning, and behavior. In: KANAREK, RB.; LIEBERMAN, H. R. Diet, Brain and Behavior: Practical Implications, p. 215-236. 2011. ALP, P.R.; NEWSHOLME, E.A.; ZAMMIT, V.A. 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Occurrence and Distribution of Fresh-Water Molluscs in the Riacho Fundo Creek Basin, Brasilia, Brazil. Revista Brasileira de Biologia v.49, n. 3-4, p. 865-870. 2001. SIMKISS, K.; MASON, A.Z. Metal ions: Metabolic and toxic effects. In: HOCHACHKA, P. W. The Mollusca. Academic Press, New York, cap.4, 1982. p. 137. SOUZA, C.P.; DRUMOND, S.C.; SILVA, C.J.E.; QUEIROZ, L.A.; GUIMARÃES, C.T.; ROCHA, R.S. Investigação sobre a transmissão da esquistossomose no complexo turístico da Serra do Cipó, MG. Iesus, v. 7, n. 4, p. 43-51. 1998. SOUZA, C.P.; CLARK, L. Moluscos de interesse parasitológico do Brasil. 2nd. Fiocruz Belo Horizonte, 1997. 73p. THOMAS, J. D.; EATON, P. The origins, fate, and ecological significance of free amino compounds released by freshwater pulmonate snails. Comparative Biochemistry and Physiology, v. 119A, n. 1, p. 341–349. 1998. TIELENS, A.G.M. Energy generation in parasitic helminths. Parasitology Today, v. 10, p. 346–352. 1994. TUNHOLI, V.M.; LUSTRINO, D.; TUNHOLI-ALVES, V.M.; MELLO-SILVA, C.C.C.; MALDONADO-JÚNIOR.; RODRIGUES, M.L.A.; PINHEIRO, J. Changes in the reproductive biology of Biomphalara glabrata infected with different doses of Echinostoma paraensei miracidia. Journal of Invertebrate Pathology, v. 106, p. 192–195. 2011. TUNHOLI, V.M.; TUNHOLI-ALVES, V.M.; LUSTRINO, D.; ROSANE, N.; CASTRO, R.N.; SANT’ANA, L.O.; GARCIA, J.S.; ARNALDO, M.A.J.; SANTOS, M.A.J.; RODRIGUES, M.L.A.; PINHEIRO, J. Aerobic to anaerobic transition in Biomphalaria glabrata (Say, 1818) infected with different miracidial doses of Echinostoma paraensei (Lie and Basch, 1967) by high-performance liquid chromatography. Experimental Parasitology, v. 133, p. 403–410. 2013. TUNHOLI-ALVES, V.M.; TUNHOLI, V.M.; GARCIA, J.S.; COSTA-NETO, S.F.; MALDONADO-JÚNIOR, A.; SANTOS, M.A.J.; THIENGO, S.C.; PINHEIRO, J. Changes in the calcium metabolism of Biomphalaria glabrata experimentally infected with Angiostrongylus cantonensis. Journal of Helminthology, p. 1- 6. 2012. VINAUD, M.C.2007. Vias do metabolismo energético e respiratório em cisticercos de Taenia crassiceps in vivo e seu estudo in vitro sob a ação de fármacos anti-helmínticos. 2007. 107f. Tese – Universidade Federal de Goiás. 2007. WEICHSELBAUM, C.T.E. An accurate and rapid method for determination of proteins in small amounts of blood serum and plasma. American Journal of Clinical Pathology, 16, p. 40–49. 1946. YOUNG, D.S. Effects of preanalytical variables on clinical laboratory tests. 1nd, Washinsgton: AACC Press. 1993. ZARAI, Z.; BOULAIS, N.; KARRAY, A.; MISERY, L.; BEZZINE, S.T.; REBAI, T.; GARGOURI1, Y.; MEJDOUB, H. Immunohistochemical localization of hepatopancreatic phospholipase A2 in Hexaplex Trunculus digestive cells. Lipids in Health and Disease, v. 10, n. 91, p. 1-9. 2011. ZELCK, U.E.; BECKER, W.; BAYNE, C.J. The plasma proteins of Biomphalaria glabrata in the presence and absence of Schistosoma mansoni. 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| dc.title.por.fl_str_mv |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| dc.title.alternative.por.fl_str_mv |
Influence of exposure to calcium carbonate in the metabolism of organic and inorganic compounds in biomphalaria glabrata, host schistosoma mansoni |
| title |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| spellingShingle |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni Silva, Luciana Damacena Biomphalaria glabrata Carbonato de cálcio Cetabolismo energético Biomphalaria glabrata Energetic metabolism Calcium carbonate PARASITOLOGIA::PROTOZOOLOGIA DE PARASITOS |
| title_short |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| title_full |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| title_fullStr |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| title_full_unstemmed |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| title_sort |
Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni |
| author |
Silva, Luciana Damacena |
| author_facet |
Silva, Luciana Damacena |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Bezerra, José Clecildo Barreto |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9491755585617846 |
| dc.contributor.advisor-co1.fl_str_mv |
Castro, Ana Maria de |
| dc.contributor.advisor-co2.fl_str_mv |
Mello-Silva, Clélia Christina Corrê de |
| dc.contributor.referee1.fl_str_mv |
Bezerra, José Clecildo Barreto |
| dc.contributor.referee2.fl_str_mv |
Castro, Ana maria de |
| dc.contributor.referee3.fl_str_mv |
Mello-Silva, Clélia Christina Corrêa de |
| dc.contributor.referee4.fl_str_mv |
Vinaud, Mariana Clare |
| dc.contributor.referee5.fl_str_mv |
Barcelos, Rejane da Silva Sena |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3800305083164308 |
| dc.contributor.author.fl_str_mv |
Silva, Luciana Damacena |
| contributor_str_mv |
Bezerra, José Clecildo Barreto Castro, Ana Maria de Mello-Silva, Clélia Christina Corrê de Bezerra, José Clecildo Barreto Castro, Ana maria de Mello-Silva, Clélia Christina Corrêa de Vinaud, Mariana Clare Barcelos, Rejane da Silva Sena |
| dc.subject.por.fl_str_mv |
Biomphalaria glabrata Carbonato de cálcio Cetabolismo energético Biomphalaria glabrata Energetic metabolism Calcium carbonate |
| topic |
Biomphalaria glabrata Carbonato de cálcio Cetabolismo energético Biomphalaria glabrata Energetic metabolism Calcium carbonate PARASITOLOGIA::PROTOZOOLOGIA DE PARASITOS |
| dc.subject.cnpq.fl_str_mv |
PARASITOLOGIA::PROTOZOOLOGIA DE PARASITOS |
| description |
Biomphalaria snails act as intermediate hosts that harbor the Schistosoma mansoni parasite, the causative agent of schistosomiasis. As population increases, snails have their geographical distribution conditioned to the presence of calcium in the environment, which is essential for their energetic metabolism. The objective of this study was the assessment on the influence of exposure to different concentrations of CaCO3 (20, 40, 60, 80 and 100 mg/L) at different intervals (1, 14, 21 and 30 days) forthe concentrations of glucose, calcium, total proteins, urea, uric acid and creatinine in hemolymph B. glabrata. All substances were dosed using specific kits with a spectrophotometer reading at 545 nm. High performance liquid chromatography (HPLC) was the technique applied to quantify the presence of organic acids: pyruvate, oxaloacetate, citrate, succinate, fumarate, propionate, acetoacetate and βhydroxybutyrate in the hemolymph of mollusks exposed to CaCO3. Results revealed that the calcium concentration in the hemolymph did not present significant difference (p>0.05) with regard to control as well as to the concentrations assessed. The glucose concentration decreased (p<0.05) in exposures to 20mg and 40mg and increased in exposures to 80mg and 100mg of CaCO3 with regard to control. The organic acids pyruvate, oxaloacetate, citrate, succinate fumarate and lactate had their concentrations increased; propionate in turn had its concentration decreased set against control in exposure to CaCO3. In the exposures to the concentrations assessed, acetoacetate decreased and β-hidroxibutirato increased. Total proteins and urea presented decrease. The uric acid concentration increased in the exposure to 20mg of CaCO3; the creatinine concentration increased in the exposures to 40mg and 100mg of CaCO3. It was possible to conclude that snails exposed to different concentrations of CaCO3 had their major metabolic alterations occurring from the 14th day of exposure. |
| publishDate |
2014 |
| dc.date.accessioned.fl_str_mv |
2014-09-19T21:28:29Z |
| dc.date.issued.fl_str_mv |
2014-03-26 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| format |
doctoralThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
Silva, Luciana Damacena. Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni. 2014. 90 f. Tese (Doutorado em Medicina Tropical e Saúde Pública) - Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) - Universidade Federal de Goiás, Goiânia, 2014. |
| dc.identifier.uri.fl_str_mv |
http://repositorio.bc.ufg.br/tede/handle/tede/3111 |
| dc.identifier.dark.fl_str_mv |
ark:/38995/001300000153c |
| identifier_str_mv |
Silva, Luciana Damacena. Influência da exposição ao carbonato de cálcio no metabolismo de compostos orgânicos e inorgânicos em biomphalaria glabrata, hospedeiro de schistosoma mansoni. 2014. 90 f. Tese (Doutorado em Medicina Tropical e Saúde Pública) - Programa de Pós-graduação em Medicina Tropical e Saúde Publica (IPTSP) - Universidade Federal de Goiás, Goiânia, 2014. ark:/38995/001300000153c |
| url |
http://repositorio.bc.ufg.br/tede/handle/tede/3111 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.program.fl_str_mv |
6085308344741430434 |
| dc.relation.confidence.fl_str_mv |
600 600 600 |
| dc.relation.department.fl_str_mv |
1545772475950486338 |
| dc.relation.cnpq.fl_str_mv |
2878476940568031721 |
| dc.relation.references.por.fl_str_mv |
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