Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/69373 |
Resumo: | Endophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well as promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of the multifunctional endophytic isolate Acinetobacter lwoffii Bac109 in promoting the early in vitro growth of sugarcane seedlings. The Bac109 strain showed potential to solubilize phosphate in a solid medium (solubilization index: 3.73). In addition, the bacterium was an efficient biocontrol agent against the phytopathogenic fungi Rhizoctonia sp., Fusarium oxysporum, Phoma sp. and Bipolaris papendorfii, showing a performance equal to or better than the commercial antifungal hygromycin B. An in vitro assay confirmed the biofilm production, which increased in the presence of sugarcane root extract. Additionally, A. lwoffii Bac109 showed a strong adhesion to the sugarcane roots. The inoculation of this bacterium in micropropagated sugarcane seedlings increased the shoot length (35 %) and regulated the nonphotochemical energy dissipation after 28 days of cultivation. At the end of the experiment, the bacterium showed a great potential for survival, with 5.72 × 107 CFU mL-1 recovered from the substrate, what is crucial for plant interaction. The results showed the potential of the biotechnology application for A. lwoffii Bac109 by evaluating multifunctional traits of plant growth promotion and by specific interactions with sugarcane, which may help to improve micropropagation protocols for this crop. KEYWORDS: Saccharum sp., biofilm, biocontrol, cross-inoculation, plant growth promoting bacteria. |
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Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcaneCaracterísticas multifuncionais de Acinetobacter lwoffii Bac109 para promoção de crescimento e colonização em cana-de-açúcar micropropagadaEndophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well as promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of the multifunctional endophytic isolate Acinetobacter lwoffii Bac109 in promoting the early in vitro growth of sugarcane seedlings. The Bac109 strain showed potential to solubilize phosphate in a solid medium (solubilization index: 3.73). In addition, the bacterium was an efficient biocontrol agent against the phytopathogenic fungi Rhizoctonia sp., Fusarium oxysporum, Phoma sp. and Bipolaris papendorfii, showing a performance equal to or better than the commercial antifungal hygromycin B. An in vitro assay confirmed the biofilm production, which increased in the presence of sugarcane root extract. Additionally, A. lwoffii Bac109 showed a strong adhesion to the sugarcane roots. The inoculation of this bacterium in micropropagated sugarcane seedlings increased the shoot length (35 %) and regulated the nonphotochemical energy dissipation after 28 days of cultivation. At the end of the experiment, the bacterium showed a great potential for survival, with 5.72 × 107 CFU mL-1 recovered from the substrate, what is crucial for plant interaction. The results showed the potential of the biotechnology application for A. lwoffii Bac109 by evaluating multifunctional traits of plant growth promotion and by specific interactions with sugarcane, which may help to improve micropropagation protocols for this crop. KEYWORDS: Saccharum sp., biofilm, biocontrol, cross-inoculation, plant growth promoting bacteria.Bactérias endofíticas com características multifuncionais podem beneficiar as plantas por meio de diferentes mecanismos e promover o crescimento de forma eficiente, econômica e ecologicamente correta. Neste estudo, foi investigado o potencial do isolado endofítico multifuncional Acinetobacter lwoffii Bac109, com o objetivo de promover o crescimento de mudas de cana-de-açúcar in vitro. A linhagem Bac109 demonstrou capacidade de solubilização de fosfato em meio sólido (índice de solubilização: 3,73). Além disso, a bactéria foi um eficiente agente de biocontrole contra os fungos fitopatogênicos Rhizoctonia sp., Fusarium oxysporum, Phoma sp. e Bipolaris papendorfii, apresentando desempenho superior ou igual ao antifúngico comercial higromicina B. A produção de biofilme foi confirmada em ensaio in vitro e foi maior na presença de extrato de raiz de cana-de-açúcar. Além disso, A. lwoffii Bac109 apresentou forte adesão às raízes da cana-de-açúcar. A inoculação da bactéria em mudas micropropagadas de cana-de-açúcar aumentou o comprimento da parte aérea (35 %) e a dissipação de energia não fotoquímica regulada após 28 dias de cultivo. Ao término do experimento, a bactéria apresentou grande potencial de sobrevivência, com 5,72 × 107 UFC mL-1 recuperadas do substrato, o que é crucial para a interação da planta. Os resultados mostraram potencial de aplicação biotecnológica para A. lwoffii Bac109 pela avaliação dos traços multifuncionais para a promoção do crescimento vegetal e por interações específicas com a cana-de-açúcar, as quais podem auxiliar no aprimoramento de protocolos de micropropagação da cultura. PALAVRAS-CHAVE: Saccharum sp., biofilme, biocontrole, inoculação cruzada, bactérias promotoras de crescimento de plantas.Escola de Agronomia - Universidade Federal de Goiás2021-10-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/69373Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69373Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69373Pesquisa Agropecuária Tropical; v. 51 (2021); e693731983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/69373/37288Copyright (c) 2021 Pesquisa Agropecuária Tropicalinfo:eu-repo/semantics/openAccessSilva, Janniffer Custódio daSantos, Luan Dionisio Silva Faria, Paula Sperotto AlbertoSilva, Fabiano GuimarãesRubio Neto, AurélioMartins, Paula FabianeSelari, Priscila Jane Romano Gonçalves2021-10-14T15:21:42Zoai:ojs.revistas.ufg.br:article/69373Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:33.203054Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane Características multifuncionais de Acinetobacter lwoffii Bac109 para promoção de crescimento e colonização em cana-de-açúcar micropropagada |
title |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
spellingShingle |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane Silva, Janniffer Custódio da |
title_short |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
title_full |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
title_fullStr |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
title_full_unstemmed |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
title_sort |
Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane |
author |
Silva, Janniffer Custódio da |
author_facet |
Silva, Janniffer Custódio da Santos, Luan Dionisio Silva Faria, Paula Sperotto Alberto Silva, Fabiano Guimarães Rubio Neto, Aurélio Martins, Paula Fabiane Selari, Priscila Jane Romano Gonçalves |
author_role |
author |
author2 |
Santos, Luan Dionisio Silva Faria, Paula Sperotto Alberto Silva, Fabiano Guimarães Rubio Neto, Aurélio Martins, Paula Fabiane Selari, Priscila Jane Romano Gonçalves |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Janniffer Custódio da Santos, Luan Dionisio Silva Faria, Paula Sperotto Alberto Silva, Fabiano Guimarães Rubio Neto, Aurélio Martins, Paula Fabiane Selari, Priscila Jane Romano Gonçalves |
description |
Endophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well as promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of the multifunctional endophytic isolate Acinetobacter lwoffii Bac109 in promoting the early in vitro growth of sugarcane seedlings. The Bac109 strain showed potential to solubilize phosphate in a solid medium (solubilization index: 3.73). In addition, the bacterium was an efficient biocontrol agent against the phytopathogenic fungi Rhizoctonia sp., Fusarium oxysporum, Phoma sp. and Bipolaris papendorfii, showing a performance equal to or better than the commercial antifungal hygromycin B. An in vitro assay confirmed the biofilm production, which increased in the presence of sugarcane root extract. Additionally, A. lwoffii Bac109 showed a strong adhesion to the sugarcane roots. The inoculation of this bacterium in micropropagated sugarcane seedlings increased the shoot length (35 %) and regulated the nonphotochemical energy dissipation after 28 days of cultivation. At the end of the experiment, the bacterium showed a great potential for survival, with 5.72 × 107 CFU mL-1 recovered from the substrate, what is crucial for plant interaction. The results showed the potential of the biotechnology application for A. lwoffii Bac109 by evaluating multifunctional traits of plant growth promotion and by specific interactions with sugarcane, which may help to improve micropropagation protocols for this crop. KEYWORDS: Saccharum sp., biofilm, biocontrol, cross-inoculation, plant growth promoting bacteria. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/69373 |
url |
https://revistas.ufg.br/pat/article/view/69373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/69373/37288 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Agropecuária Tropical |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 51 (2021); e69373 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 51 (2021); e69373 Pesquisa Agropecuária Tropical; v. 51 (2021); e69373 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874821106958336 |