SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
Texto Completo: | https://revistas.ufg.br/vet/article/view/e-31292 |
Resumo: | This study compared six dog cadavers, with no predilection for breed, age, or sex, weighing between 2 and 10 kg, from the Environmental Monitoring Directory of the Federal District, Brazil. Animals were divided into three random groups. The vascular systems were cleaned with supersaturated saline, modified Larssen solution, and common water, respectively, followed by preservation with modified Larssen solution at 10% of body weight, aiming to determine the durability as a model for surgical training. Surgeries were performed in the period between six successive freezing and thawing cycles to evaluate the resistance, color, odor, flexibility and degree of tissue autolysis through histological analysis of heart, liver, spleen, intestine, and brain. All the groups showed good quality for surgical training, allowing cavity organs surgeries until the third thawing, bulbar conjunctiva surgeries until the fourth thawing, orthopedic surgeries for up to the sixth thawing, becoming unworkable from this point, due to the high degree of tissue autolysis. The group of cadavers preserved with supersaturated saline solution stood out by hardly giving off unpleasant smell; in addition, brain and heart are the only organs that practically did not show macroscopic and microscopic changes in the first three thawing cycles.Keywords: Alternative teaching method; Animal ethics; Modified Larsson solution; Surgical training |
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SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERSComparação cirúrgica e histológica de cadáveres quimicamente preservados e criopreservadosThis study compared six dog cadavers, with no predilection for breed, age, or sex, weighing between 2 and 10 kg, from the Environmental Monitoring Directory of the Federal District, Brazil. Animals were divided into three random groups. The vascular systems were cleaned with supersaturated saline, modified Larssen solution, and common water, respectively, followed by preservation with modified Larssen solution at 10% of body weight, aiming to determine the durability as a model for surgical training. Surgeries were performed in the period between six successive freezing and thawing cycles to evaluate the resistance, color, odor, flexibility and degree of tissue autolysis through histological analysis of heart, liver, spleen, intestine, and brain. All the groups showed good quality for surgical training, allowing cavity organs surgeries until the third thawing, bulbar conjunctiva surgeries until the fourth thawing, orthopedic surgeries for up to the sixth thawing, becoming unworkable from this point, due to the high degree of tissue autolysis. The group of cadavers preserved with supersaturated saline solution stood out by hardly giving off unpleasant smell; in addition, brain and heart are the only organs that practically did not show macroscopic and microscopic changes in the first three thawing cycles.Keywords: Alternative teaching method; Animal ethics; Modified Larsson solution; Surgical trainingEste trabalho comparou seis cadáveres de cães, sem predileção por raça, idade ou sexo, com peso entre 2 e 10 kg, provenientes da Diretoria de Vigilância Ambiental do Distrito Federal. Os cadáveres, cujos sistemas vasculares foram limpos com solução salina supersaturada, solução de Larssen modificada e água comum, foram divididos em três grupos aleatórios, respectivamente. EM seguida foram conservados com solução de Larssen modificada na proporção de 10% de seu peso corporal, objetivando-se terminar a durabilidade como modelo para o treinamento cirúrgico. Após seis sucessivos congelamentos e descongelamentos, nos quais foram realizadas cirurgias visando avaliar a resistência, cor, odor, maleabilidade e grau de autólise tecidual através de análises histológicas de coração, fígado, baço, intestino e encéfalo, ficou determinado que todos os grupos apresentaram boa qualidade para treinamento cirúrgico, permitindo cirurgias de órgãos cavitários até o terceiro descongelamento, cirurgias da conjuntiva bulbar por até quatro descongelamentos e cirurgias ortopédicas por até seis descongelamentos, ficando inviáveis a partir deste ponto, devido ao elevado grau de autólise tecidual. O grupo conservado com solução salina supersaturada se destacou por quase não desprender odor desagradável, além do encéfalo e do coração serem os únicos órgãos que praticamente não apresentaram alterações macro e microscópicas nos três primeiros descongelamentos.Palavras-chave: Ética animal; Método alternativo de ensino; Solução de Larssen modificada; Treinamento cirúrgicoUniversidade Federal de Goiás2018-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ufg.br/vet/article/view/e-3129210.1590/cab19031292Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-14Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-141809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/e-31292/25955https://revistas.ufg.br/vet/article/view/e-31292/25956Alvarenga dos Santos, BrunoDiniz Galera, PaulaAdjuto Eloi, RômuloIsmar Santana, Marceloinfo:eu-repo/semantics/openAccess2023-03-23T18:50:39Zoai:ojs.revistas.ufg.br:article/31292Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:06.886568Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS Comparação cirúrgica e histológica de cadáveres quimicamente preservados e criopreservados |
title |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
spellingShingle |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS Alvarenga dos Santos, Bruno |
title_short |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
title_full |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
title_fullStr |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
title_full_unstemmed |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
title_sort |
SURGICAL AND HISTOLOGICAL COMPARISON OF CHEMICALLY PRESERVED AND CRYOPRESERVED CADAVERS |
author |
Alvarenga dos Santos, Bruno |
author_facet |
Alvarenga dos Santos, Bruno Diniz Galera, Paula Adjuto Eloi, Rômulo Ismar Santana, Marcelo |
author_role |
author |
author2 |
Diniz Galera, Paula Adjuto Eloi, Rômulo Ismar Santana, Marcelo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Alvarenga dos Santos, Bruno Diniz Galera, Paula Adjuto Eloi, Rômulo Ismar Santana, Marcelo |
description |
This study compared six dog cadavers, with no predilection for breed, age, or sex, weighing between 2 and 10 kg, from the Environmental Monitoring Directory of the Federal District, Brazil. Animals were divided into three random groups. The vascular systems were cleaned with supersaturated saline, modified Larssen solution, and common water, respectively, followed by preservation with modified Larssen solution at 10% of body weight, aiming to determine the durability as a model for surgical training. Surgeries were performed in the period between six successive freezing and thawing cycles to evaluate the resistance, color, odor, flexibility and degree of tissue autolysis through histological analysis of heart, liver, spleen, intestine, and brain. All the groups showed good quality for surgical training, allowing cavity organs surgeries until the third thawing, bulbar conjunctiva surgeries until the fourth thawing, orthopedic surgeries for up to the sixth thawing, becoming unworkable from this point, due to the high degree of tissue autolysis. The group of cadavers preserved with supersaturated saline solution stood out by hardly giving off unpleasant smell; in addition, brain and heart are the only organs that practically did not show macroscopic and microscopic changes in the first three thawing cycles.Keywords: Alternative teaching method; Animal ethics; Modified Larsson solution; Surgical training |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-31292 10.1590/cab19031292 |
url |
https://revistas.ufg.br/vet/article/view/e-31292 |
identifier_str_mv |
10.1590/cab19031292 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/e-31292/25955 https://revistas.ufg.br/vet/article/view/e-31292/25956 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 19 (2018): Continuous publication; 1-14 Ciência Animal Brasileira / Brazilian Animal Science; v. 19 (2018): Publicação contínua; 1-14 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1799874788695474176 |