Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por eng |
| Título da fonte: | Ciência animal brasileira (Online) |
| Texto Completo: | https://revistas.ufg.br/vet/article/view/72715 |
Resumo: | Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases. Keywords: bone neoplasms; D-17; natural compounds; therapeutic safety |
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Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferationExtrato etanólico de açafrão (Curcuma longa L.) reduz apoptose e promove proliferação de células de osteossarcoma caninoCurcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases. Keywords: bone neoplasms; D-17; natural compounds; therapeutic safetyA Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 μM, 20 μM, 50 μM, 100 μM e 1000 μM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50μM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.Palavras-chave: compostos naturais; D-17; neoplasias ósseas; segurança terapêuticaUniversidade Federal de Goiás2022-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistas.ufg.br/vet/article/view/72715Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publicationCiência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua1809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporenghttps://revistas.ufg.br/vet/article/view/72715/38734https://revistas.ufg.br/vet/article/view/72715/38914https://revistas.ufg.br/vet/article/view/72715/38735https://revistas.ufg.br/vet/article/view/72715/38915Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMoura Alves, Naira de Sousa Cruz, VanessaLopes Nepomuceno, LeandroPeixoto Soares, NayaneArnhold, EmmanuelGraziani, Danielde Almeida Machado Gonçalves, PatríciaSalazar Badan, Guilherme Henriqueda Matta Santos, AmandaAraújo, Eugênio Gonçalves de2023-03-23T18:43:54Zoai:ojs.revistas.ufg.br:article/72715Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:56:31.302790Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
| dc.title.none.fl_str_mv |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation Extrato etanólico de açafrão (Curcuma longa L.) reduz apoptose e promove proliferação de células de osteossarcoma canino |
| title |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| spellingShingle |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation Moura Alves, Naira |
| title_short |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| title_full |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| title_fullStr |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| title_full_unstemmed |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| title_sort |
Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation |
| author |
Moura Alves, Naira |
| author_facet |
Moura Alves, Naira de Sousa Cruz, Vanessa Lopes Nepomuceno, Leandro Peixoto Soares, Nayane Arnhold, Emmanuel Graziani, Daniel de Almeida Machado Gonçalves, Patrícia Salazar Badan, Guilherme Henrique da Matta Santos, Amanda Araújo, Eugênio Gonçalves de |
| author_role |
author |
| author2 |
de Sousa Cruz, Vanessa Lopes Nepomuceno, Leandro Peixoto Soares, Nayane Arnhold, Emmanuel Graziani, Daniel de Almeida Machado Gonçalves, Patrícia Salazar Badan, Guilherme Henrique da Matta Santos, Amanda Araújo, Eugênio Gonçalves de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moura Alves, Naira de Sousa Cruz, Vanessa Lopes Nepomuceno, Leandro Peixoto Soares, Nayane Arnhold, Emmanuel Graziani, Daniel de Almeida Machado Gonçalves, Patrícia Salazar Badan, Guilherme Henrique da Matta Santos, Amanda Araújo, Eugênio Gonçalves de |
| description |
Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases. Keywords: bone neoplasms; D-17; natural compounds; therapeutic safety |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09-15 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/72715 |
| url |
https://revistas.ufg.br/vet/article/view/72715 |
| dc.language.iso.fl_str_mv |
por eng |
| language |
por eng |
| dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/72715/38734 https://revistas.ufg.br/vet/article/view/72715/38914 https://revistas.ufg.br/vet/article/view/72715/38735 https://revistas.ufg.br/vet/article/view/72715/38915 |
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Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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Copyright (c) 2022 Ciência Animal Brasileira / Brazilian Animal Science http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
| publisher.none.fl_str_mv |
Universidade Federal de Goiás |
| dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 23 (2022): Continuous publication Ciência Animal Brasileira / Brazilian Animal Science; v. 23 (2022): Publicação contínua 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
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Universidade Federal de Goiás (UFG) |
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UFG |
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UFG |
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Ciência animal brasileira (Online) |
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Ciência animal brasileira (Online) |
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Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
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||revistacab@gmail.com |
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1799874790687768576 |