EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS

Detalhes bibliográficos
Autor(a) principal: Zimmermann, Marina Ferreira
Data de Publicação: 2009
Outros Autores: Farinasso, Adalberto, Melo, Cely Marini, Papa, Frederico Ozanam, Neves, Jairo Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/1932
Resumo: The purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P0.05) on membrane integrity between treatments was not observed.KEY WORDS: Cryoprotectant,  diluent, equine, freezing, thawing.
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spelling EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERSSOBREVIDA DAS CÉLULAS ESPERMÁTICAS EQUINAS CRIOPRESERVADAS APÓS DESCONGELAÇÃO E DILUIÇÃO UTILIZANDO-SE DOIS DILUENTES COMERCIAISReprodução AnimalThe purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P0.05) on membrane integrity between treatments was not observed.KEY WORDS: Cryoprotectant,  diluent, equine, freezing, thawing.O presente estudo teve por objetivo avaliar parâmetros de motilidade e viabilidade in vitro na diluição do crioprotetor dimetil-formamida a 5% pós-descongelação para concentrações de 2,5% e 1,25%, mediante utilização de dois diluentes comerciais adicionados junto ao sêmen. Após a descongelação, as amostras foram diluídas com a finalidade de manter as concentrações finais (2,5% e 1,25%) de crioprotetor, utilizando-se dois diluentes comerciais (FR4® e Botu-Crio®) em dois tempos: inicial (Ti) e final (Tf).  Empregaram-se quinze amostras de ejaculados distintos de cinco garanhões de raças nacionais. Os parâmetros de motilidade foram observados através da análise computadorizada e os de integridade de membrana plasmática pela microscopia de epifluorescência. Verificou-se melhora nos parâmetros de motilidade total e progressiva dos espermatozóides, no tempo final (P<0,05) com o diluente Botu-Crio® em relação ao FR4®. Não houve diferença (P>0,05) entre os tratamentos quanto à integridade de membrana plasmática.PALAVRAS-CHAVES: Crioprotetor, congelação, descongelação, diluente, equino. Universidade Federal de Goiás2009-04-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresPesquisa Científicaapplication/pdfhttps://revistas.ufg.br/vet/article/view/193210.5216/cab.v10i1.1932Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 1 (2009); 238-245Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 1 (2009); 238-2451809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1932/4687Zimmermann, Marina FerreiraFarinasso, AdalbertoMelo, Cely MariniPapa, Frederico OzanamNeves, Jairo Pereirainfo:eu-repo/semantics/openAccess2013-10-31T10:46:34Zoai:ojs.revistas.ufg.br:article/1932Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:00.017640Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
SOBREVIDA DAS CÉLULAS ESPERMÁTICAS EQUINAS CRIOPRESERVADAS APÓS DESCONGELAÇÃO E DILUIÇÃO UTILIZANDO-SE DOIS DILUENTES COMERCIAIS
title EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
spellingShingle EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
Zimmermann, Marina Ferreira
Reprodução Animal
title_short EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
title_full EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
title_fullStr EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
title_full_unstemmed EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
title_sort EQUINE FROZEN SPERMATIC CELL SURVIVOR AFTER THAWED AND DILUTION USING TWO DIFFERENT COMMERCIAL EXTENDERS
author Zimmermann, Marina Ferreira
author_facet Zimmermann, Marina Ferreira
Farinasso, Adalberto
Melo, Cely Marini
Papa, Frederico Ozanam
Neves, Jairo Pereira
author_role author
author2 Farinasso, Adalberto
Melo, Cely Marini
Papa, Frederico Ozanam
Neves, Jairo Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zimmermann, Marina Ferreira
Farinasso, Adalberto
Melo, Cely Marini
Papa, Frederico Ozanam
Neves, Jairo Pereira
dc.subject.por.fl_str_mv Reprodução Animal
topic Reprodução Animal
description The purpose of this study was to evaluate the motility and viability of stallion semen cryopreserved with 5% dimethyl-formamyde as cryoprotectant and posterior dilution to 2.5 and 1.25% as final concentration of cryoprotectant. After thawing, samples were diluted with two commercial extenders (FR4® and Botucrio® ) in two moments: initial (Ti) and final (Tf). A total of 15 distinct ejaculates from five stallions of national breeds were analysed. Motility was observed using a computer assistant system analysis and viability was analyzed using fluorescent probes. A significant (P0.05) on membrane integrity between treatments was not observed.KEY WORDS: Cryoprotectant,  diluent, equine, freezing, thawing.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
Pesquisa Científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/1932
10.5216/cab.v10i1.1932
url https://revistas.ufg.br/vet/article/view/1932
identifier_str_mv 10.5216/cab.v10i1.1932
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/1932/4687
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 1 (2009); 238-245
Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 1 (2009); 238-245
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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