MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS

Detalhes bibliográficos
Autor(a) principal: Chirinéa, Viviane Helena
Data de Publicação: 2006
Outros Autores: Martins, Maria Isabel Mello, Souza, Fabiana Ferreira de, Tebet, Jussara Maria, Papa, Frederico Ozanan, Lopes, Maria Denise
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/870
Resumo: The objective of this study was to verify the efficiency of two different extenders: TRIS/Fructose/Citric Acid/Glycerol (8 %) - (TRIS 8 %) (Morton, 1988 modified) and commercial extender - MP50) (PAPA et al., 2002) – for freezing dog semen. Ten ejaculates from different adult dogs were collected by digital manipulation. The samples semen were evaluated for sperm motility and vigor, hypo-osmotic swelling test, sperm membrane integrity, sperm morphology, ultra structural analysis in three different moments, fresh (T1), cooled (T2) and thawed (T3). The samples were packaged in 0.5 mL French straws with 40 x 106 spermatozoa/ straw, and kept at 5 0C for 60 minutes (T2); then frozen in static vapor of nitrogen for the following 20 minutes and immersed in liquid nitrogen until being thawed in 70 0C water for 8 seconds (T3). By analysis of variance, it would be possible to verify the animal effect on almost all variables observed in this study, except for sperm motility and membrane integrity. For cooled semen (T2), MP50 were significantly better for hypo-osmotic swelling test, sperm membrane integrity (p
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spelling MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERSCARACTERÍSTICAS MORFOFUNCIONAIS DO SÊMEN CANINO REFRIGERADO E CONGELADO, USANDO DOIS DIFERENTES MEIOS DILUENTESThe objective of this study was to verify the efficiency of two different extenders: TRIS/Fructose/Citric Acid/Glycerol (8 %) - (TRIS 8 %) (Morton, 1988 modified) and commercial extender - MP50) (PAPA et al., 2002) – for freezing dog semen. Ten ejaculates from different adult dogs were collected by digital manipulation. The samples semen were evaluated for sperm motility and vigor, hypo-osmotic swelling test, sperm membrane integrity, sperm morphology, ultra structural analysis in three different moments, fresh (T1), cooled (T2) and thawed (T3). The samples were packaged in 0.5 mL French straws with 40 x 106 spermatozoa/ straw, and kept at 5 0C for 60 minutes (T2); then frozen in static vapor of nitrogen for the following 20 minutes and immersed in liquid nitrogen until being thawed in 70 0C water for 8 seconds (T3). By analysis of variance, it would be possible to verify the animal effect on almost all variables observed in this study, except for sperm motility and membrane integrity. For cooled semen (T2), MP50 were significantly better for hypo-osmotic swelling test, sperm membrane integrity (pO objetivo deste estudo foi avaliar, por meio de análise funcional e morfológica, dois meios diluentes – TRIS/ frutose/ácido cítrico/glicerol (8 %) (TRIS 8 %) (Morton, 1988 modificado) e um meio comercial (MP50) (PAPA et al., 2002) – para criopreservação de sêmen canino. Colheramse dez ejaculados de cães adultos, por manipulação digital do pênis. Avaliaram-se as amostras pela motilidade espermática, velocidade espermática, teste hiposmótico, integridade de membrana espermática, morfologia espermática, e análise ultra-estrutural no sêmen fresco (T1), refrigerado (T2) e descongelado (T3). Envasaram-se as amostras diluídas em palhetas francesas de 0,5 mL, com 40 x 106 espermatozóides/palheta e se as mantiveram por sessenta minutos a 5 ºC (T2); em seguida, transferiram-nas para vapor de nitrogênio líquido durante vinte minutos e posteriormente estocadas. O sêmen foi descongelado a 70 ºC por 8 segundos. A análise de variância mostrou influência do animal nas diferentes variáveis, com exceção da motilidade espermática e integridade de membrana espermática. Na refrigeração (T2), o meio MP50 apresentou melhores resultados no teste hiposmótico e na integridade de membrana (p0,05). A análise ultra-estrutural do sêmen mostrou edema e ondulação da membrana plasmática e acrossomal nas diferentes etapas do processo de criopreservação. Conclui-se que os meios diluentes utilizados mostraram ser semelhantes quanto às características morfofuncionais após a descongelação. PALAVRAS-CHAVE: Cão, congelação, meio diluente, sêmen.Universidade Federal de Goiás2006-12-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/87010.5216/cab.v7i4.870Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 7 No. 4 (2006); 407-415Ciência Animal Brasileira / Brazilian Animal Science; v. 7 n. 4 (2006); 407-4151809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/870/1009Chirinéa, Viviane HelenaMartins, Maria Isabel MelloSouza, Fabiana Ferreira deTebet, Jussara MariaPapa, Frederico OzananLopes, Maria Deniseinfo:eu-repo/semantics/openAccess2007-02-05T11:15:17Zoai:ojs.revistas.ufg.br:article/870Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:45.372156Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
CARACTERÍSTICAS MORFOFUNCIONAIS DO SÊMEN CANINO REFRIGERADO E CONGELADO, USANDO DOIS DIFERENTES MEIOS DILUENTES
title MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
spellingShingle MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
Chirinéa, Viviane Helena
title_short MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
title_full MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
title_fullStr MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
title_full_unstemmed MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
title_sort MORPHOFUNCTIONAL CHARACTERISTICS OF CANINE COOLED AND FROZEN SEMEN USING TWO DIFFERENT EXTENDERS
author Chirinéa, Viviane Helena
author_facet Chirinéa, Viviane Helena
Martins, Maria Isabel Mello
Souza, Fabiana Ferreira de
Tebet, Jussara Maria
Papa, Frederico Ozanan
Lopes, Maria Denise
author_role author
author2 Martins, Maria Isabel Mello
Souza, Fabiana Ferreira de
Tebet, Jussara Maria
Papa, Frederico Ozanan
Lopes, Maria Denise
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chirinéa, Viviane Helena
Martins, Maria Isabel Mello
Souza, Fabiana Ferreira de
Tebet, Jussara Maria
Papa, Frederico Ozanan
Lopes, Maria Denise
description The objective of this study was to verify the efficiency of two different extenders: TRIS/Fructose/Citric Acid/Glycerol (8 %) - (TRIS 8 %) (Morton, 1988 modified) and commercial extender - MP50) (PAPA et al., 2002) – for freezing dog semen. Ten ejaculates from different adult dogs were collected by digital manipulation. The samples semen were evaluated for sperm motility and vigor, hypo-osmotic swelling test, sperm membrane integrity, sperm morphology, ultra structural analysis in three different moments, fresh (T1), cooled (T2) and thawed (T3). The samples were packaged in 0.5 mL French straws with 40 x 106 spermatozoa/ straw, and kept at 5 0C for 60 minutes (T2); then frozen in static vapor of nitrogen for the following 20 minutes and immersed in liquid nitrogen until being thawed in 70 0C water for 8 seconds (T3). By analysis of variance, it would be possible to verify the animal effect on almost all variables observed in this study, except for sperm motility and membrane integrity. For cooled semen (T2), MP50 were significantly better for hypo-osmotic swelling test, sperm membrane integrity (p
publishDate 2006
dc.date.none.fl_str_mv 2006-12-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/870
10.5216/cab.v7i4.870
url https://revistas.ufg.br/vet/article/view/870
identifier_str_mv 10.5216/cab.v7i4.870
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/870/1009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 7 No. 4 (2006); 407-415
Ciência Animal Brasileira / Brazilian Animal Science; v. 7 n. 4 (2006); 407-415
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
_version_ 1799874782742708224

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