Protoplast production from napier grass and pearl millet triploid hybrids
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência e Agrotecnologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020 |
Resumo: | The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time. |
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Ciência e Agrotecnologia (Online) |
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Protoplast production from napier grass and pearl millet triploid hybridsPennisetum purpureumPennisetum glaucumIn vitro cultivationInterspecific hybridsForageThe objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.Editora da UFLA2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020Ciência e Agrotecnologia v.34 n.5 2010reponame:Ciência e Agrotecnologia (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLA10.1590/S1413-70542010000500020info:eu-repo/semantics/openAccessTimbó,Ana Luiza de OliveiraDavide,Lisete ChammaPinto,José Eduardo Brasil PereiraPereira,Antônio Vandereng2010-11-29T00:00:00Zoai:scielo:S1413-70542010000500020Revistahttp://www.scielo.br/cagroPUBhttps://old.scielo.br/oai/scielo-oai.php||renpaiva@dbi.ufla.br|| editora@editora.ufla.br1981-18291413-7054opendoar:2022-11-22T16:30:58.472152Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA)true |
dc.title.none.fl_str_mv |
Protoplast production from napier grass and pearl millet triploid hybrids |
title |
Protoplast production from napier grass and pearl millet triploid hybrids |
spellingShingle |
Protoplast production from napier grass and pearl millet triploid hybrids Timbó,Ana Luiza de Oliveira Pennisetum purpureum Pennisetum glaucum In vitro cultivation Interspecific hybrids Forage |
title_short |
Protoplast production from napier grass and pearl millet triploid hybrids |
title_full |
Protoplast production from napier grass and pearl millet triploid hybrids |
title_fullStr |
Protoplast production from napier grass and pearl millet triploid hybrids |
title_full_unstemmed |
Protoplast production from napier grass and pearl millet triploid hybrids |
title_sort |
Protoplast production from napier grass and pearl millet triploid hybrids |
author |
Timbó,Ana Luiza de Oliveira |
author_facet |
Timbó,Ana Luiza de Oliveira Davide,Lisete Chamma Pinto,José Eduardo Brasil Pereira Pereira,Antônio Vander |
author_role |
author |
author2 |
Davide,Lisete Chamma Pinto,José Eduardo Brasil Pereira Pereira,Antônio Vander |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Timbó,Ana Luiza de Oliveira Davide,Lisete Chamma Pinto,José Eduardo Brasil Pereira Pereira,Antônio Vander |
dc.subject.por.fl_str_mv |
Pennisetum purpureum Pennisetum glaucum In vitro cultivation Interspecific hybrids Forage |
topic |
Pennisetum purpureum Pennisetum glaucum In vitro cultivation Interspecific hybrids Forage |
description |
The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-70542010000500020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da UFLA |
publisher.none.fl_str_mv |
Editora da UFLA |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.34 n.5 2010 reponame:Ciência e Agrotecnologia (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Ciência e Agrotecnologia (Online) |
collection |
Ciência e Agrotecnologia (Online) |
repository.name.fl_str_mv |
Ciência e Agrotecnologia (Online) - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
||renpaiva@dbi.ufla.br|| editora@editora.ufla.br |
_version_ |
1799874967716757504 |