The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/42634 |
Resumo: | Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process. |
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The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteriaInsoluble phosphatePlant growth-promoting rhizobacteriaSolubilization haloMicrodropFosfato insolúvelRizobactérias promotoras de crescimento de plantasHalo de solubilizaçãoAgar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.Universidade Estadual de Maringá2020-08-25T11:52:01Z2020-08-25T11:52:01Z2020-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332.http://repositorio.ufla.br/jspui/handle/1/42634Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessOliveira-Longatti, Silvia Maria deMarra, Leandro MarcianoCarvalho, Teotonio Soares deMoreira, Fatima Maria de Souzaeng2020-08-25T11:53:48Zoai:localhost:1/42634Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-08-25T11:53:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
title |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
spellingShingle |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria Oliveira-Longatti, Silvia Maria de Insoluble phosphate Plant growth-promoting rhizobacteria Solubilization halo Microdrop Fosfato insolúvel Rizobactérias promotoras de crescimento de plantas Halo de solubilização |
title_short |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
title_full |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
title_fullStr |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
title_full_unstemmed |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
title_sort |
The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria |
author |
Oliveira-Longatti, Silvia Maria de |
author_facet |
Oliveira-Longatti, Silvia Maria de Marra, Leandro Marciano Carvalho, Teotonio Soares de Moreira, Fatima Maria de Souza |
author_role |
author |
author2 |
Marra, Leandro Marciano Carvalho, Teotonio Soares de Moreira, Fatima Maria de Souza |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira-Longatti, Silvia Maria de Marra, Leandro Marciano Carvalho, Teotonio Soares de Moreira, Fatima Maria de Souza |
dc.subject.por.fl_str_mv |
Insoluble phosphate Plant growth-promoting rhizobacteria Solubilization halo Microdrop Fosfato insolúvel Rizobactérias promotoras de crescimento de plantas Halo de solubilização |
topic |
Insoluble phosphate Plant growth-promoting rhizobacteria Solubilization halo Microdrop Fosfato insolúvel Rizobactérias promotoras de crescimento de plantas Halo de solubilização |
description |
Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-25T11:52:01Z 2020-08-25T11:52:01Z 2020-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332. http://repositorio.ufla.br/jspui/handle/1/42634 |
identifier_str_mv |
OLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332. |
url |
http://repositorio.ufla.br/jspui/handle/1/42634 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835197897441280 |