The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria

Detalhes bibliográficos
Autor(a) principal: Oliveira-Longatti, Silvia Maria de
Data de Publicação: 2020
Outros Autores: Marra, Leandro Marciano, Carvalho, Teotonio Soares de, Moreira, Fatima Maria de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/42634
Resumo: Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.
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spelling The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteriaInsoluble phosphatePlant growth-promoting rhizobacteriaSolubilization haloMicrodropFosfato insolúvelRizobactérias promotoras de crescimento de plantasHalo de solubilizaçãoAgar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.Universidade Estadual de Maringá2020-08-25T11:52:01Z2020-08-25T11:52:01Z2020-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332.http://repositorio.ufla.br/jspui/handle/1/42634Acta Scientiarum. Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessOliveira-Longatti, Silvia Maria deMarra, Leandro MarcianoCarvalho, Teotonio Soares deMoreira, Fatima Maria de Souzaeng2020-08-25T11:53:48Zoai:localhost:1/42634Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-08-25T11:53:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
spellingShingle The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
Oliveira-Longatti, Silvia Maria de
Insoluble phosphate
Plant growth-promoting rhizobacteria
Solubilization halo
Microdrop
Fosfato insolúvel
Rizobactérias promotoras de crescimento de plantas
Halo de solubilização
title_short The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_full The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_fullStr The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_full_unstemmed The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
title_sort The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria
author Oliveira-Longatti, Silvia Maria de
author_facet Oliveira-Longatti, Silvia Maria de
Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
author_role author
author2 Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira-Longatti, Silvia Maria de
Marra, Leandro Marciano
Carvalho, Teotonio Soares de
Moreira, Fatima Maria de Souza
dc.subject.por.fl_str_mv Insoluble phosphate
Plant growth-promoting rhizobacteria
Solubilization halo
Microdrop
Fosfato insolúvel
Rizobactérias promotoras de crescimento de plantas
Halo de solubilização
topic Insoluble phosphate
Plant growth-promoting rhizobacteria
Solubilization halo
Microdrop
Fosfato insolúvel
Rizobactérias promotoras de crescimento de plantas
Halo de solubilização
description Agar media with insoluble phosphates are used for semi-quantitative screening of phosphate-solubilizing bacteria based on the solubilization halo formed around the colonies. We show that the volume of the culture medium (15, 20, and 30 mL) and the inoculation method (toothpick vs microdrop) strongly influence the diameter of the solubilization halo, and this should be considered in advance selection of the isolates most efficient in this process.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-25T11:52:01Z
2020-08-25T11:52:01Z
2020-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332.
http://repositorio.ufla.br/jspui/handle/1/42634
identifier_str_mv OLIVEIRA-LONGATTI, S. M. de et al. The culture medium volume and the inoculation method should be considered in semi-quantitative screening of calcium phosphate solubilization by bacteria. Acta Scientiarum. Agronomy, Maringá, v. 42, e44332, 2020. DOI: 10.4025/actasciagron.v42i1.44332.
url http://repositorio.ufla.br/jspui/handle/1/42634
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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