Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains

Detalhes bibliográficos
Autor(a) principal: Viana, Roberta Oliveira
Data de Publicação: 2019
Outros Autores: Magalhães-Guedes, Karina Teixeira, Dias, Disney Ribeiro, Schwan, Rosane Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39855
Resumo: The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.
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spelling Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grainsUtilização do biossensor Maldi-Tof MS na avaliação de micro-organismos dos grãos de kefir brasileiroMicroorganismsBacteriaYeastBiological sensorMicro-organismosBactériasLeveduraSensor biológicoThe aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.O objetivo deste estudo foi avaliar o uso do biossensor de Maldi-Tof MS na avaliação microbiana de grãos de kefir. Maldi-Tof MS é uma nova metodologia para o diagnóstico rápido de micro-organismos. Um total de 358 microorganismos foram isolados, 31 foram leveduras e 327 foram bactérias (divididas em bactérias láticas e acéticas). Colônias microbianas foram cultivadas em meio Luria-Bertani (LB) e incubadas a 35 °C por 18h e posteriormente utilizadas na identificação a nível de espécies pelo Maldi-Tof MS. A população microbiana identificada nos grãos de kefir brasileiros foi Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus e Acetobacter syzygii. Este estudo demonstrou uma identificação rápida e precisa dos micro-organismos de grãos de kefir brasileiros utilizando o biossensor Maldi-Tof MS. Em conclusão, a tecnologia Maldi-Tof MS pode facilitar o controle microbiológico em um processo de fermentação usando grãos de kefir como culturas iniciadoras.Universidade Federal de Viçosa2020-04-07T12:03:28Z2020-04-07T12:03:28Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019.http://repositorio.ufla.br/jspui/handle/1/39855Ceresreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessViana, Roberta OliveiraMagalhães-Guedes, Karina TeixeiraDias, Disney RibeiroSchwan, Rosane Freitaseng2020-04-07T12:03:28Zoai:localhost:1/39855Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-04-07T12:03:28Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
Utilização do biossensor Maldi-Tof MS na avaliação de micro-organismos dos grãos de kefir brasileiro
title Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
spellingShingle Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
Viana, Roberta Oliveira
Microorganisms
Bacteria
Yeast
Biological sensor
Micro-organismos
Bactérias
Levedura
Sensor biológico
title_short Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_full Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_fullStr Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_full_unstemmed Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_sort Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
author Viana, Roberta Oliveira
author_facet Viana, Roberta Oliveira
Magalhães-Guedes, Karina Teixeira
Dias, Disney Ribeiro
Schwan, Rosane Freitas
author_role author
author2 Magalhães-Guedes, Karina Teixeira
Dias, Disney Ribeiro
Schwan, Rosane Freitas
author2_role author
author
author
dc.contributor.author.fl_str_mv Viana, Roberta Oliveira
Magalhães-Guedes, Karina Teixeira
Dias, Disney Ribeiro
Schwan, Rosane Freitas
dc.subject.por.fl_str_mv Microorganisms
Bacteria
Yeast
Biological sensor
Micro-organismos
Bactérias
Levedura
Sensor biológico
topic Microorganisms
Bacteria
Yeast
Biological sensor
Micro-organismos
Bactérias
Levedura
Sensor biológico
description The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-04-07T12:03:28Z
2020-04-07T12:03:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv VIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019.
http://repositorio.ufla.br/jspui/handle/1/39855
identifier_str_mv VIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019.
url http://repositorio.ufla.br/jspui/handle/1/39855
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Ceres
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835153703108608

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