Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/39855 |
Resumo: | The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures. |
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Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grainsUtilização do biossensor Maldi-Tof MS na avaliação de micro-organismos dos grãos de kefir brasileiroMicroorganismsBacteriaYeastBiological sensorMicro-organismosBactériasLeveduraSensor biológicoThe aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.O objetivo deste estudo foi avaliar o uso do biossensor de Maldi-Tof MS na avaliação microbiana de grãos de kefir. Maldi-Tof MS é uma nova metodologia para o diagnóstico rápido de micro-organismos. Um total de 358 microorganismos foram isolados, 31 foram leveduras e 327 foram bactérias (divididas em bactérias láticas e acéticas). Colônias microbianas foram cultivadas em meio Luria-Bertani (LB) e incubadas a 35 °C por 18h e posteriormente utilizadas na identificação a nível de espécies pelo Maldi-Tof MS. A população microbiana identificada nos grãos de kefir brasileiros foi Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus e Acetobacter syzygii. Este estudo demonstrou uma identificação rápida e precisa dos micro-organismos de grãos de kefir brasileiros utilizando o biossensor Maldi-Tof MS. Em conclusão, a tecnologia Maldi-Tof MS pode facilitar o controle microbiológico em um processo de fermentação usando grãos de kefir como culturas iniciadoras.Universidade Federal de Viçosa2020-04-07T12:03:28Z2020-04-07T12:03:28Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019.http://repositorio.ufla.br/jspui/handle/1/39855Ceresreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessViana, Roberta OliveiraMagalhães-Guedes, Karina TeixeiraDias, Disney RibeiroSchwan, Rosane Freitaseng2020-04-07T12:03:28Zoai:localhost:1/39855Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-04-07T12:03:28Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains Utilização do biossensor Maldi-Tof MS na avaliação de micro-organismos dos grãos de kefir brasileiro |
title |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
spellingShingle |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains Viana, Roberta Oliveira Microorganisms Bacteria Yeast Biological sensor Micro-organismos Bactérias Levedura Sensor biológico |
title_short |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
title_full |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
title_fullStr |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
title_full_unstemmed |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
title_sort |
Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains |
author |
Viana, Roberta Oliveira |
author_facet |
Viana, Roberta Oliveira Magalhães-Guedes, Karina Teixeira Dias, Disney Ribeiro Schwan, Rosane Freitas |
author_role |
author |
author2 |
Magalhães-Guedes, Karina Teixeira Dias, Disney Ribeiro Schwan, Rosane Freitas |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Viana, Roberta Oliveira Magalhães-Guedes, Karina Teixeira Dias, Disney Ribeiro Schwan, Rosane Freitas |
dc.subject.por.fl_str_mv |
Microorganisms Bacteria Yeast Biological sensor Micro-organismos Bactérias Levedura Sensor biológico |
topic |
Microorganisms Bacteria Yeast Biological sensor Micro-organismos Bactérias Levedura Sensor biológico |
description |
The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by MaldiTof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020-04-07T12:03:28Z 2020-04-07T12:03:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019. http://repositorio.ufla.br/jspui/handle/1/39855 |
identifier_str_mv |
VIANA, R. O. et al. Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Ceres, Viçosa, MG, v. 66, n. 1, p. 72-76, Jan./Feb. 2019. |
url |
http://repositorio.ufla.br/jspui/handle/1/39855 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
publisher.none.fl_str_mv |
Universidade Federal de Viçosa |
dc.source.none.fl_str_mv |
Ceres reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835153703108608 |