Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp

Detalhes bibliográficos
Autor(a) principal: Timbó, Ana Luiza de Oliveira
Data de Publicação: 2012
Outros Autores: Pereira, Roselaine Cristina, Santos, Vanderley Borges dos, Sobrinho, Fausto Souza, Davide, Lisete Chamma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39010
Resumo: Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5), coefficient of variation and estimation of DNA content. The best combination for the analysis of Brachiaria spp. DNA content was the use of MgSO4 buffer with R. sativus as a internal reference standard. Genome sizes expressed in picograms of DNA are presented for all genotypes and the importance of the histogram score on the results reliability of DNA content analyses were discussed.
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spelling Histogram score contributes for reliability of DNA content estimatives in Brachiaria sppNotas do histograma contribuem para a confiabilidade das estimativas do conteúdo de DNA de Brachiaria sppBrachiaria ruziziensisBrachiaria brizanthaColchicineConteúdo de DNA nuclearColchicinaFlow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5), coefficient of variation and estimation of DNA content. The best combination for the analysis of Brachiaria spp. DNA content was the use of MgSO4 buffer with R. sativus as a internal reference standard. Genome sizes expressed in picograms of DNA are presented for all genotypes and the importance of the histogram score on the results reliability of DNA content analyses were discussed.A citometria de fluxo permite estimar o conteúdo de DNA de um grande número de plantas rapidamente. No entanto, protocolos inadequados podem comprometer a confiabilidade dessas estimativas, levando a variações nos valores de conteúdo de DNA para uma mesma espécie. Neste trabalho, objetivou-se propor um protocolo eficiente para a estimativa do conteúdo de DNA de genótipos de Brachiaria spp. com diferentes níveis de ploidia, utilizando a citometria de fluxo. Foram avaliados quatro genótipos (B. ruziziensis, diploide e tetraploidizada artificialmente; B. brizantha tetraploide e um híbrido natural triploide), 3 soluções tampões (MgSO4, Galbraith e Tris-HCl) e três espécies como padrões de referência interno (Raphanus sativus, Solanum lycopersicum e Pisum sativum). As variáveis mensuradas foram: nota do histograma (1 a 5), coeficiente de variação e estimativa do conteúdo de DNA. A melhor combinação para as análises do conteúdo de DNA de Brachiaria spp. por citometria de fluxo foi o emprego do tampão MgSO4 com o rabanete como padrão de referência. O tamanho dos genomas expresso em picogramas de DNA foi apresentado para todos os genótipos e a importância da pontuação do histograma na confiabilidade dos resultados das análises do conteúdo de DNA foi discutida.Universidade Federal de Lavras2020-02-12T14:54:53Z2020-02-12T14:54:53Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTIMBÓ, A. L. de O.; PEREIRA, R. C.; SANTOS, V. B. dos; SOBRINHO, F. S.; DAVIDE, L. C. Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp. Ciência e Agrotecnologia, Lavras, v. 36, n. 6, p. 599-607, nov./dez. 2012.http://repositorio.ufla.br/jspui/handle/1/39010Ciência e Agrotecnologiareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessTimbó, Ana Luiza de OliveiraPereira, Roselaine CristinaSantos, Vanderley Borges dosSobrinho, Fausto SouzaDavide, Lisete Chammaeng2020-02-12T14:54:54Zoai:localhost:1/39010Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-02-12T14:54:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
Notas do histograma contribuem para a confiabilidade das estimativas do conteúdo de DNA de Brachiaria spp
title Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
spellingShingle Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
Timbó, Ana Luiza de Oliveira
Brachiaria ruziziensis
Brachiaria brizantha
Colchicine
Conteúdo de DNA nuclear
Colchicina
title_short Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
title_full Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
title_fullStr Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
title_full_unstemmed Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
title_sort Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp
author Timbó, Ana Luiza de Oliveira
author_facet Timbó, Ana Luiza de Oliveira
Pereira, Roselaine Cristina
Santos, Vanderley Borges dos
Sobrinho, Fausto Souza
Davide, Lisete Chamma
author_role author
author2 Pereira, Roselaine Cristina
Santos, Vanderley Borges dos
Sobrinho, Fausto Souza
Davide, Lisete Chamma
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Timbó, Ana Luiza de Oliveira
Pereira, Roselaine Cristina
Santos, Vanderley Borges dos
Sobrinho, Fausto Souza
Davide, Lisete Chamma
dc.subject.por.fl_str_mv Brachiaria ruziziensis
Brachiaria brizantha
Colchicine
Conteúdo de DNA nuclear
Colchicina
topic Brachiaria ruziziensis
Brachiaria brizantha
Colchicine
Conteúdo de DNA nuclear
Colchicina
description Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5), coefficient of variation and estimation of DNA content. The best combination for the analysis of Brachiaria spp. DNA content was the use of MgSO4 buffer with R. sativus as a internal reference standard. Genome sizes expressed in picograms of DNA are presented for all genotypes and the importance of the histogram score on the results reliability of DNA content analyses were discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012
2020-02-12T14:54:53Z
2020-02-12T14:54:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv TIMBÓ, A. L. de O.; PEREIRA, R. C.; SANTOS, V. B. dos; SOBRINHO, F. S.; DAVIDE, L. C. Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp. Ciência e Agrotecnologia, Lavras, v. 36, n. 6, p. 599-607, nov./dez. 2012.
http://repositorio.ufla.br/jspui/handle/1/39010
identifier_str_mv TIMBÓ, A. L. de O.; PEREIRA, R. C.; SANTOS, V. B. dos; SOBRINHO, F. S.; DAVIDE, L. C. Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp. Ciência e Agrotecnologia, Lavras, v. 36, n. 6, p. 599-607, nov./dez. 2012.
url http://repositorio.ufla.br/jspui/handle/1/39010
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
publisher.none.fl_str_mv Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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