Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata

Detalhes bibliográficos
Autor(a) principal: Bertolucci, Suzan Kelly V.
Data de Publicação: 2013
Outros Autores: Pereira, Ana B. D., Pinto, José E. B. P., Oliveira, A. B., Braga, Fernão C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38321
Resumo: The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profiles and contents of coumarin (1), trans-o-coumaric (2), kaurenoic (3), benzoylgrandifloric (4) and cinnamoylgrandifloric (5) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2, the constituents were isolated and purified to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata. trans-o-Coumaric was not detected in both, whereas 1 occurred only in M. laevigata. The concentrations of the assayed constituents did not vary significantly within the evaluated period (p < 0.05), for both species. In contrast, changes in the chromatographic profiles and spectral purity of peaks from 3, 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profile in M. laevigata modified after six months of storage. The evaluation of chromatographic profiles based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers' contents.
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spelling Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerataChemical markers changesMikania laevigataMikania glomerataAlterações de marcadores químicosThe leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profiles and contents of coumarin (1), trans-o-coumaric (2), kaurenoic (3), benzoylgrandifloric (4) and cinnamoylgrandifloric (5) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2, the constituents were isolated and purified to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata. trans-o-Coumaric was not detected in both, whereas 1 occurred only in M. laevigata. The concentrations of the assayed constituents did not vary significantly within the evaluated period (p < 0.05), for both species. In contrast, changes in the chromatographic profiles and spectral purity of peaks from 3, 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profile in M. laevigata modified after six months of storage. The evaluation of chromatographic profiles based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers' contents.As folhas de Mikania laevigata e Mikania glomerata são usadas no Brasil para tratar afecções respiratórias, sendo diterpernos caur ânicos e cumarina considerados os constituintes bioativos. O presente estudo relata a investigação dos perfis por CLAE-DAD e os teores de cumarina (1), ácidos trans-o-coumárico (2), caurenóico (3), benzoilgrandiflórico (4) e cinamoilgrandiflórico (5) em folhas secas de espécies de Mikania armazenadas em sala escura, sob condições controladas. Com exceção de 2, os constituintes foram isolados e purificados para serem empregados como subst âncias de referência. As amostras foram analisadas trimestralmente, até um período de 18 meses para M. laevigata e 12 meses para M. glomerata. Ácido trans-o-coumárico não foi detectado em ambas as espécies, enquanto 1 foi encontrado somente em M. laevigata. As concentrações dos constituintes quantificados não variaram significativamente no período avaliado (p < 0,05), para duas espécies. Por outro lado, alterações nos perfis cromatográficos e na pureza espectral dos picos relativos a 3, 4 e 5 foram detectadas em amostras de ambas as espécies de Mikania armazenadas por três meses, enquanto o perfil do pico correspondente a cumarina mostrou-se alterado em M. laevigata a partir do sexto mês de armazenamento. A análise dos perfis cromatográficos baseada na avaliação da pureza espectral dos picos selecionados mostrou-se uma ferramenta mais robusta para acessar a estabilidade química de amostras de Mikania submetidas a armazenamento do que a quantificação do teor dos marcadores químicos.Academia Brasileira de Ciências2019-12-19T17:19:19Z2019-12-19T17:19:19Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBERTOLUCCI, S. K. V.; PEREIRA, A. B. D.; PINTO, J. E. B. P.; OLIVEIRA, A. B.; BRAGA, F. C. Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 2, p. 473-485, 2013.http://repositorio.ufla.br/jspui/handle/1/38321Anais da Academia Brasileira de Ciênciasreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBertolucci, Suzan Kelly V.Pereira, Ana B. D.Pinto, José E. B. P.Oliveira, A. B.Braga, Fernão C.eng2023-05-26T18:51:23Zoai:localhost:1/38321Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:51:23Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
title Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
spellingShingle Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
Bertolucci, Suzan Kelly V.
Chemical markers changes
Mikania laevigata
Mikania glomerata
Alterações de marcadores químicos
title_short Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
title_full Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
title_fullStr Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
title_full_unstemmed Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
title_sort Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata
author Bertolucci, Suzan Kelly V.
author_facet Bertolucci, Suzan Kelly V.
Pereira, Ana B. D.
Pinto, José E. B. P.
Oliveira, A. B.
Braga, Fernão C.
author_role author
author2 Pereira, Ana B. D.
Pinto, José E. B. P.
Oliveira, A. B.
Braga, Fernão C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bertolucci, Suzan Kelly V.
Pereira, Ana B. D.
Pinto, José E. B. P.
Oliveira, A. B.
Braga, Fernão C.
dc.subject.por.fl_str_mv Chemical markers changes
Mikania laevigata
Mikania glomerata
Alterações de marcadores químicos
topic Chemical markers changes
Mikania laevigata
Mikania glomerata
Alterações de marcadores químicos
description The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profiles and contents of coumarin (1), trans-o-coumaric (2), kaurenoic (3), benzoylgrandifloric (4) and cinnamoylgrandifloric (5) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2, the constituents were isolated and purified to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata. trans-o-Coumaric was not detected in both, whereas 1 occurred only in M. laevigata. The concentrations of the assayed constituents did not vary significantly within the evaluated period (p < 0.05), for both species. In contrast, changes in the chromatographic profiles and spectral purity of peaks from 3, 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profile in M. laevigata modified after six months of storage. The evaluation of chromatographic profiles based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers' contents.
publishDate 2013
dc.date.none.fl_str_mv 2013
2019-12-19T17:19:19Z
2019-12-19T17:19:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BERTOLUCCI, S. K. V.; PEREIRA, A. B. D.; PINTO, J. E. B. P.; OLIVEIRA, A. B.; BRAGA, F. C. Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 2, p. 473-485, 2013.
http://repositorio.ufla.br/jspui/handle/1/38321
identifier_str_mv BERTOLUCCI, S. K. V.; PEREIRA, A. B. D.; PINTO, J. E. B. P.; OLIVEIRA, A. B.; BRAGA, F. C. Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 85, n. 2, p. 473-485, 2013.
url http://repositorio.ufla.br/jspui/handle/1/38321
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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