Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon

Detalhes bibliográficos
Autor(a) principal: Sousa , Fernanda Maria de
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/49823
Resumo: Due to the growing demand for foods that are beneficial to health and with low environmental impact, the fermentation industry has been betting on studies involving alternative plant sources such as agricultural processing residues, such as coffee pulp, and PANC's (Unconventional Food Plants), like jerivá almonds. The objective of this project was to optimize the fermentation process of a medium based on extracts of arabica coffee pulp (EPC), jerivá almonds (EAJ) and yacon flour (FrY) with probiotics, through a sequential planning strategy applying initially the Plackett and Burman and then the DCCR (Central Composite Rotational Design). 50 mL of different formulations were fermented in triplicate with Lactobacillus rhamnosus and Bifidobacterium longum at 37ºC in anaerobic conditions, brought to pH≈4.5 to obtain a sensorially balanced drink. After analysis by Plackett and Burman, it was concluded that the most important independent variables were EPC and FrY, as they reduced the fermentation time of the symbiotic drink (t≈20h), with good cell growth (≈5 LogUFC.mL-1) , consumption of total (CAT) and reducing (CAR) sugars (max=68.0 g/L and 43.2 g/L, respectively), production of exopolysaccharides (max=0.9 g/L) and organic acids ( max=1.08g/L). The DCCR results indicated that the response variables Time; TotalGrow; GrowLR; GrowBL; CAT; PAT and Color parameters L*, a*, b* showed a good fit of the model at 95% confidence (p<0.05). The best prototypes of symbiotic drink contained 10 to 14 % w/v of Yacon Flour, 8 to 16 % v/v of coffee pulp extract, 5% v/v of functional coffee pulp extract and 17.5 % v/v of jerivá almond extract, with the best cell counts (≈13 LogUFC.mL-1). By validating the models under these conditions, it was possible to predict the fermentation time (around 20h) and CresTotal (around 5.1 Log CFU.mL-1); CrescLR (around 5.3 LogUFC.mL-1) and CrescBL (around 5.02 LogUFC.mL-1). Of the DCCR treatments, the one that showed the highest production of lactic acid (5.3 g/L) (HPLC) and the highest concentration of live cells (48.45%) (Flow Cytometry) at the end of fermentation was Treatment 4, (22.1%m/v of FrY and 25.6%v/v of EPC), with postbiotic compounds being identified in all treatments evaluated with a maximum of 26.15% in Treatment 5 (5%m/v of FrY and 15 %v/v EPC), which guarantees beverage functionality. In vitro functional tests showed higher probiotic counts in the first 3 hours of digestion (8.1 LogUFC.mL-1) with a drastic drop after 4 hours of digestion (5.5 LogUFC.mL-1) and the ability to Cell adherence to gut-associated lymphoid tissue ranged from low (%H=32.07 Treatment 1 of Validation) to medium (%H=39.31 of Treatment 2 of Validation).
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spelling Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yaconFormulation and functionality tests of symbiotic beverage based on coffee pulp, jerivah almonds and yacon flourProbióticosFermentação de extratos vegetaisLactobacillus rhamnosusBifidobacterium longumProcesso de fermentaçãoPolpa de caféProbioticsFermentation of vegetable extractsFermentation processCoffee pulpEngenharia de AlimentosDue to the growing demand for foods that are beneficial to health and with low environmental impact, the fermentation industry has been betting on studies involving alternative plant sources such as agricultural processing residues, such as coffee pulp, and PANC's (Unconventional Food Plants), like jerivá almonds. The objective of this project was to optimize the fermentation process of a medium based on extracts of arabica coffee pulp (EPC), jerivá almonds (EAJ) and yacon flour (FrY) with probiotics, through a sequential planning strategy applying initially the Plackett and Burman and then the DCCR (Central Composite Rotational Design). 50 mL of different formulations were fermented in triplicate with Lactobacillus rhamnosus and Bifidobacterium longum at 37ºC in anaerobic conditions, brought to pH≈4.5 to obtain a sensorially balanced drink. After analysis by Plackett and Burman, it was concluded that the most important independent variables were EPC and FrY, as they reduced the fermentation time of the symbiotic drink (t≈20h), with good cell growth (≈5 LogUFC.mL-1) , consumption of total (CAT) and reducing (CAR) sugars (max=68.0 g/L and 43.2 g/L, respectively), production of exopolysaccharides (max=0.9 g/L) and organic acids ( max=1.08g/L). The DCCR results indicated that the response variables Time; TotalGrow; GrowLR; GrowBL; CAT; PAT and Color parameters L*, a*, b* showed a good fit of the model at 95% confidence (p<0.05). The best prototypes of symbiotic drink contained 10 to 14 % w/v of Yacon Flour, 8 to 16 % v/v of coffee pulp extract, 5% v/v of functional coffee pulp extract and 17.5 % v/v of jerivá almond extract, with the best cell counts (≈13 LogUFC.mL-1). By validating the models under these conditions, it was possible to predict the fermentation time (around 20h) and CresTotal (around 5.1 Log CFU.mL-1); CrescLR (around 5.3 LogUFC.mL-1) and CrescBL (around 5.02 LogUFC.mL-1). Of the DCCR treatments, the one that showed the highest production of lactic acid (5.3 g/L) (HPLC) and the highest concentration of live cells (48.45%) (Flow Cytometry) at the end of fermentation was Treatment 4, (22.1%m/v of FrY and 25.6%v/v of EPC), with postbiotic compounds being identified in all treatments evaluated with a maximum of 26.15% in Treatment 5 (5%m/v of FrY and 15 %v/v EPC), which guarantees beverage functionality. In vitro functional tests showed higher probiotic counts in the first 3 hours of digestion (8.1 LogUFC.mL-1) with a drastic drop after 4 hours of digestion (5.5 LogUFC.mL-1) and the ability to Cell adherence to gut-associated lymphoid tissue ranged from low (%H=32.07 Treatment 1 of Validation) to medium (%H=39.31 of Treatment 2 of Validation).Devido à crescente demanda por alimentos benéficos a saúde e ainda com baixo impacto ambiental, a indústria do ramo fermentativo vem apostando em estudos que envolvam fontes vegetais alternativas como resíduos de processamento agrícola, exemplo a polpa do café, e PANC’s (Plantas Alimentícias Não Convencionais), como as amêndoas do jerivá. O objetivo deste projeto foi otimizar o processo de fermentação de meio à base de extratos da polpa de café arábica (EPC), amêndoas do jerivá (EAJ) e farinha de yacon (FrY) com probióticos, através de uma estratégia sequencial de planejamentos aplicando inicialmente o Plackett e Burman e em seguida o DCCR (Delineamento Composto Central Rotacional). Foram fermentados 50 mL de diferentes formulações em triplicata com Lactobacillus rhamnosus e Bifidobacterium longum a 37ºC em anaerobiose, conduzidas até pH≈4,5 para obtenção de uma bebida sensorialmente equilibrada. Após análise por Plackett e Burman, concluiu-se que, as variáveis independentes mais importantes foram EPC e FrY, por diminuírem o tempo de fermentação da bebida simbiótica (t≈20h), com bom crescimento celular (≈5 LogUFC.mL-1), consumo de açúcares totais (CAT) e redutores (CAR) (máx=68,0 g/L e 43,2 g/L, respectivamente), produção de exopolissacarídeos (máx=0,9 g/L) e ácidos orgânicos (máx= 1,08g/L). Os resultados do DCCR indicaram que as variáveis respostas Tempo; CrescTotal; CrescLR; CrescBL; CAT; PAT e Parâmetros de cor L*, a*, b* apresentaram bom ajuste do modelo a 95% de confiança (p<0,05). Os melhores protótipos de bebida simbiótica continham de 10 a 14 % m/v de Farinha de Yacon, 8 a 16 %v/v de extrato de polpa de café, 5% v/v de extrato de polpa de café funcional e 17,5% v/v de extrato de amêndoas de jerivá, com as melhores contagens de células (≈13 LogUFC.mL-1). Através da validação dos modelos nessas condições, foi possível predizer o tempo de fermentação (em torno de 20h) e os CresTotal (em torno de 5,1 Log UFC.mL-1); CrescLR (em torno de 5,3 LogUFC.mL-1) e CrescBL (em torno de 5,02 LogUFC.mL-1). Dos tratamentos do DCCR, o que apresentou maior produção de ácido lático (5,3 g/L) (HPLC) e maior concentração de células vivas (48,45%) (Citometria de Fluxo) ao final da fermentação foi o Tratamento 4, (22,1%m/v de FrY e 25,6%v/v de EPC), sendo identificados compostos pós-bióticos em todos os tratamentos avaliados com máximo de 26,15% no Tratamento 5 (5 %m/v de FrY e 15 %v/v de EPC), o que garante funcionalidades a bebida. Dos testes funcionais in vitro notou-se maiores contagens de probióticos nas primeiras 3 horas de digestão (8,1 LogUFC.mL-1) com queda drástica após 4 horas de digestão (5,5 LogUFC.mL-1) e a capacidade de aderência das células ao tecido linfóide associado ao intestino variaram de baixa (%H=32,07 Tratamento 1 da Validação) a média (%H=39,31 do Tratamento 2 da Validação).Universidade Federal de LavrasPrograma de Pós-Graduação em Engenharia de AlimentosUFLAbrasilEscola de Ciências Agrárias de LavrasMondragón-Bernal, Olga LucíaAlves, José Guilherme Lembi FerreiraMondragón-Bernal, Olga LuciaMenezes, Evandro Galvão TavaresBorém, Flávio MeiraSousa , Fernanda Maria de2022-04-28T21:19:47Z2022-04-28T21:19:47Z2022-04-282022-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfSOUSA, F. M. de. Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon. 2022. 109 p. Dissertação (Mestrado em Engenharia de Alimentos) – Universidade Federal de Lavras, Lavras, 2022.http://repositorio.ufla.br/jspui/handle/1/49823porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-04-26T21:03:34Zoai:localhost:1/49823Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-26T21:03:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
Formulation and functionality tests of symbiotic beverage based on coffee pulp, jerivah almonds and yacon flour
title Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
spellingShingle Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
Sousa , Fernanda Maria de
Probióticos
Fermentação de extratos vegetais
Lactobacillus rhamnosus
Bifidobacterium longum
Processo de fermentação
Polpa de café
Probiotics
Fermentation of vegetable extracts
Fermentation process
Coffee pulp
Engenharia de Alimentos
title_short Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
title_full Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
title_fullStr Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
title_full_unstemmed Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
title_sort Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon
author Sousa , Fernanda Maria de
author_facet Sousa , Fernanda Maria de
author_role author
dc.contributor.none.fl_str_mv Mondragón-Bernal, Olga Lucía
Alves, José Guilherme Lembi Ferreira
Mondragón-Bernal, Olga Lucia
Menezes, Evandro Galvão Tavares
Borém, Flávio Meira
dc.contributor.author.fl_str_mv Sousa , Fernanda Maria de
dc.subject.por.fl_str_mv Probióticos
Fermentação de extratos vegetais
Lactobacillus rhamnosus
Bifidobacterium longum
Processo de fermentação
Polpa de café
Probiotics
Fermentation of vegetable extracts
Fermentation process
Coffee pulp
Engenharia de Alimentos
topic Probióticos
Fermentação de extratos vegetais
Lactobacillus rhamnosus
Bifidobacterium longum
Processo de fermentação
Polpa de café
Probiotics
Fermentation of vegetable extracts
Fermentation process
Coffee pulp
Engenharia de Alimentos
description Due to the growing demand for foods that are beneficial to health and with low environmental impact, the fermentation industry has been betting on studies involving alternative plant sources such as agricultural processing residues, such as coffee pulp, and PANC's (Unconventional Food Plants), like jerivá almonds. The objective of this project was to optimize the fermentation process of a medium based on extracts of arabica coffee pulp (EPC), jerivá almonds (EAJ) and yacon flour (FrY) with probiotics, through a sequential planning strategy applying initially the Plackett and Burman and then the DCCR (Central Composite Rotational Design). 50 mL of different formulations were fermented in triplicate with Lactobacillus rhamnosus and Bifidobacterium longum at 37ºC in anaerobic conditions, brought to pH≈4.5 to obtain a sensorially balanced drink. After analysis by Plackett and Burman, it was concluded that the most important independent variables were EPC and FrY, as they reduced the fermentation time of the symbiotic drink (t≈20h), with good cell growth (≈5 LogUFC.mL-1) , consumption of total (CAT) and reducing (CAR) sugars (max=68.0 g/L and 43.2 g/L, respectively), production of exopolysaccharides (max=0.9 g/L) and organic acids ( max=1.08g/L). The DCCR results indicated that the response variables Time; TotalGrow; GrowLR; GrowBL; CAT; PAT and Color parameters L*, a*, b* showed a good fit of the model at 95% confidence (p<0.05). The best prototypes of symbiotic drink contained 10 to 14 % w/v of Yacon Flour, 8 to 16 % v/v of coffee pulp extract, 5% v/v of functional coffee pulp extract and 17.5 % v/v of jerivá almond extract, with the best cell counts (≈13 LogUFC.mL-1). By validating the models under these conditions, it was possible to predict the fermentation time (around 20h) and CresTotal (around 5.1 Log CFU.mL-1); CrescLR (around 5.3 LogUFC.mL-1) and CrescBL (around 5.02 LogUFC.mL-1). Of the DCCR treatments, the one that showed the highest production of lactic acid (5.3 g/L) (HPLC) and the highest concentration of live cells (48.45%) (Flow Cytometry) at the end of fermentation was Treatment 4, (22.1%m/v of FrY and 25.6%v/v of EPC), with postbiotic compounds being identified in all treatments evaluated with a maximum of 26.15% in Treatment 5 (5%m/v of FrY and 15 %v/v EPC), which guarantees beverage functionality. In vitro functional tests showed higher probiotic counts in the first 3 hours of digestion (8.1 LogUFC.mL-1) with a drastic drop after 4 hours of digestion (5.5 LogUFC.mL-1) and the ability to Cell adherence to gut-associated lymphoid tissue ranged from low (%H=32.07 Treatment 1 of Validation) to medium (%H=39.31 of Treatment 2 of Validation).
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T21:19:47Z
2022-04-28T21:19:47Z
2022-04-28
2022-02-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUSA, F. M. de. Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon. 2022. 109 p. Dissertação (Mestrado em Engenharia de Alimentos) – Universidade Federal de Lavras, Lavras, 2022.
http://repositorio.ufla.br/jspui/handle/1/49823
identifier_str_mv SOUSA, F. M. de. Formulação e testes de funcionalidade de bebida simbiótica a base de polpa de café, amêndoas de jerivá e farinha de yacon. 2022. 109 p. Dissertação (Mestrado em Engenharia de Alimentos) – Universidade Federal de Lavras, Lavras, 2022.
url http://repositorio.ufla.br/jspui/handle/1/49823
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Engenharia de Alimentos
UFLA
brasil
Escola de Ciências Agrárias de Lavras
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Engenharia de Alimentos
UFLA
brasil
Escola de Ciências Agrárias de Lavras
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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