Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/15201 |
Resumo: | This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies. |
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Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytesGenotoxicidadeAlimentos - Análise toxicológicaFarinha de feijão brancoTeste do cometaGenotoxicityFood - Toxicological analysisWhite Bean FlourComet assayThis study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies.Este estudo foi realizado para avaliar o potencial de indução de efeitos genotóxicos da farinha de feijão branco utilizando o teste do Cometa. O ensaio foi realizado com linfócitos humanos presentes no sangue imediatamente após a coleta, por incubação com farinha de feijão branco em três concentrações (3,92, 9,52 e 18,18 mg/mL) a 37 ºC por 4 h, seguida de preparação das lâminas. As amostras foram consideradas positivas (acima de 20% de dano), quando os danos observados no DNA celular foram maiores do que o controle negativo. Verificou-se que as doses testadas não mostraram potencial genotóxico. No entanto, seria prematuro fazer recomendações sobre o padrão de riscos para a saúde humana resultantes de danos ao DNA já que exposição das células ao extrato foi restrito ao período de quatro horas e não durante um ciclo celular completo. Além disso, outras informações sobre a toxicologia devem ser obtidas no futuro.Universidade de São Paulo, Faculdade de Ciências Farmacêuticas2017-08-15T19:21:14Z2017-08-15T19:21:14Z2012-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPEREIRA, L. L. S. et al. Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 48, n. 1, p. 103-108, jan./mar. 2012.http://repositorio.ufla.br/jspui/handle/1/15201Brazilian Journal of Pharmaceutical Sciencesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAPereira, Luciana Lopes SilvaMarcussi, SilvanaSátiro, Lívia CabralPereira, Chrystian AraujoAndrade, Larissa FonsecaDavide, Lisete ChammaSantos, Custódio Donizete dosinfo:eu-repo/semantics/openAccesseng2017-08-15T19:21:14Zoai:localhost:1/15201Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2017-08-15T19:21:14Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
title |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
spellingShingle |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes Pereira, Luciana Lopes Silva Genotoxicidade Alimentos - Análise toxicológica Farinha de feijão branco Teste do cometa Genotoxicity Food - Toxicological analysis White Bean Flour Comet assay |
title_short |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
title_full |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
title_fullStr |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
title_full_unstemmed |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
title_sort |
Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes |
author |
Pereira, Luciana Lopes Silva |
author_facet |
Pereira, Luciana Lopes Silva Marcussi, Silvana Sátiro, Lívia Cabral Pereira, Chrystian Araujo Andrade, Larissa Fonseca Davide, Lisete Chamma Santos, Custódio Donizete dos |
author_role |
author |
author2 |
Marcussi, Silvana Sátiro, Lívia Cabral Pereira, Chrystian Araujo Andrade, Larissa Fonseca Davide, Lisete Chamma Santos, Custódio Donizete dos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Luciana Lopes Silva Marcussi, Silvana Sátiro, Lívia Cabral Pereira, Chrystian Araujo Andrade, Larissa Fonseca Davide, Lisete Chamma Santos, Custódio Donizete dos |
dc.subject.por.fl_str_mv |
Genotoxicidade Alimentos - Análise toxicológica Farinha de feijão branco Teste do cometa Genotoxicity Food - Toxicological analysis White Bean Flour Comet assay |
topic |
Genotoxicidade Alimentos - Análise toxicológica Farinha de feijão branco Teste do cometa Genotoxicity Food - Toxicological analysis White Bean Flour Comet assay |
description |
This study was conducted to evaluate the potential induction of genotoxic effects of white bean flour using the Comet assay. The test was conducted with human lymphocytes present in whole blood immediately after collection, by incubation with white bean flour in three concentrations (3.92, 9.52 and 18.18 mg/mL) at 37 ºC for 4 h followed by preparation of slides. Samples were considered positive (above 20% damage) when the damage observed to cellular DNA was higher than the negative control. No genotoxic potential was found at the doses tested. However, it would be premature to suggest absence of risk to human health of DNA damage since the exposure of cells to the extract was restricted to four hours rather than a whole cell cycle. Additionally, further information on toxicology should be obtained in future studies. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 2017-08-15T19:21:14Z 2017-08-15T19:21:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PEREIRA, L. L. S. et al. Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 48, n. 1, p. 103-108, jan./mar. 2012. http://repositorio.ufla.br/jspui/handle/1/15201 |
identifier_str_mv |
PEREIRA, L. L. S. et al. Application of Comet assay to assess the effects of white bean meal on DNA of human lymphocytes. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 48, n. 1, p. 103-108, jan./mar. 2012. |
url |
http://repositorio.ufla.br/jspui/handle/1/15201 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835165146218496 |