Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive

Detalhes bibliográficos
Autor(a) principal: Micheli, Maurizio
Data de Publicação: 2018
Outros Autores: Silva, Daniel Fernandes da, Farinelli, Daniela, Agate, Graziana, Pio, Rafael, Famiani, Franco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/34792
Resumo: Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.
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spelling Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in oliveOlea europaea L.Olive - MicropropagationOlive - In vitro cultureIn vitro cultivationMicropropagationShoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.American Society for Horticultural Science (ASHS)2019-06-17T18:54:12Z2019-06-17T18:54:12Z2018-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17.http://repositorio.ufla.br/jspui/handle/1/34792Hortsciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMicheli, MaurizioSilva, Daniel Fernandes daFarinelli, DanielaAgate, GrazianaPio, RafaelFamiani, Francoeng2023-05-26T18:44:44Zoai:localhost:1/34792Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:44:44Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
title Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
spellingShingle Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
Micheli, Maurizio
Olea europaea L.
Olive - Micropropagation
Olive - In vitro culture
In vitro cultivation
Micropropagation
title_short Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
title_full Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
title_fullStr Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
title_full_unstemmed Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
title_sort Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
author Micheli, Maurizio
author_facet Micheli, Maurizio
Silva, Daniel Fernandes da
Farinelli, Daniela
Agate, Graziana
Pio, Rafael
Famiani, Franco
author_role author
author2 Silva, Daniel Fernandes da
Farinelli, Daniela
Agate, Graziana
Pio, Rafael
Famiani, Franco
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Micheli, Maurizio
Silva, Daniel Fernandes da
Farinelli, Daniela
Agate, Graziana
Pio, Rafael
Famiani, Franco
dc.subject.por.fl_str_mv Olea europaea L.
Olive - Micropropagation
Olive - In vitro culture
In vitro cultivation
Micropropagation
topic Olea europaea L.
Olive - Micropropagation
Olive - In vitro culture
In vitro cultivation
Micropropagation
description Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
2019-06-17T18:54:12Z
2019-06-17T18:54:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17.
http://repositorio.ufla.br/jspui/handle/1/34792
identifier_str_mv MICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17.
url http://repositorio.ufla.br/jspui/handle/1/34792
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Horticultural Science (ASHS)
publisher.none.fl_str_mv American Society for Horticultural Science (ASHS)
dc.source.none.fl_str_mv Hortscience
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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