Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/34792 |
Resumo: | Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration. |
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Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in oliveOlea europaea L.Olive - MicropropagationOlive - In vitro cultureIn vitro cultivationMicropropagationShoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration.American Society for Horticultural Science (ASHS)2019-06-17T18:54:12Z2019-06-17T18:54:12Z2018-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17.http://repositorio.ufla.br/jspui/handle/1/34792Hortsciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMicheli, MaurizioSilva, Daniel Fernandes daFarinelli, DanielaAgate, GrazianaPio, RafaelFamiani, Francoeng2023-05-26T18:44:44Zoai:localhost:1/34792Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:44:44Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
title |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
spellingShingle |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive Micheli, Maurizio Olea europaea L. Olive - Micropropagation Olive - In vitro culture In vitro cultivation Micropropagation |
title_short |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
title_full |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
title_fullStr |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
title_full_unstemmed |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
title_sort |
Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive |
author |
Micheli, Maurizio |
author_facet |
Micheli, Maurizio Silva, Daniel Fernandes da Farinelli, Daniela Agate, Graziana Pio, Rafael Famiani, Franco |
author_role |
author |
author2 |
Silva, Daniel Fernandes da Farinelli, Daniela Agate, Graziana Pio, Rafael Famiani, Franco |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Micheli, Maurizio Silva, Daniel Fernandes da Farinelli, Daniela Agate, Graziana Pio, Rafael Famiani, Franco |
dc.subject.por.fl_str_mv |
Olea europaea L. Olive - Micropropagation Olive - In vitro culture In vitro cultivation Micropropagation |
topic |
Olea europaea L. Olive - Micropropagation Olive - In vitro culture In vitro cultivation Micropropagation |
description |
Shoots of the olive cultivar Moraiolo were previously cultured in aseptic conditions on Olive Medium (OM), with the addition of 4 mg·L−1 of zeatin, 30 g·L−1 of sucrose, and 7 g·L−1 of agar. Then, 1-cm long uninodal explants with two leaves and two axillary buds were excised from the proliferated masses and placed on the same proliferation medium enriched with four concentrations of neem oil (0—control, 0.1, 0.5, and 1.0 mL·L−1), added before sterilization. The addition of 0.1 mL·L−1 of neem oil to the medium gave an improvement in shoot regeneration. More vigorous shoots (longer proliferated shoots) were obtained along with a higher number of nodes (multiplication rate). Overall, there was a significant increase in the total fresh and dry proliferated weights. To our knowledge, this is the first report showing a strong and beneficial effect of neem oil, used as a “complex mixture,” on in vitro plant regeneration. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04 2019-06-17T18:54:12Z 2019-06-17T18:54:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17. http://repositorio.ufla.br/jspui/handle/1/34792 |
identifier_str_mv |
MICHELI, M. et al. Neem oil used as a “complex mixture” to improve in vitro shoot proliferation in olive. Hortscience, [S.l.], v. 53, n. 4, p. 531-534, Apr. 2018. DOI: 10.21273/HORTSCI12731-17. |
url |
http://repositorio.ufla.br/jspui/handle/1/34792 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Horticultural Science (ASHS) |
publisher.none.fl_str_mv |
American Society for Horticultural Science (ASHS) |
dc.source.none.fl_str_mv |
Hortscience reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835124223442944 |