Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis

Detalhes bibliográficos
Autor(a) principal: Koshikumo, Erika Sayuri Maneti
Data de Publicação: 2014
Outros Autores: Alves, Eduardo, Von Pinho, Renzo Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28548
Resumo: Cercosporin is a phytotoxin with toxic activity against large number of organism, it is produced by hypha in the culture medium. This way, the objective of this study was to induce the production of Cercosporin in Cercospora zeina (CZ) and Cercospora sorghi f. sp. maydis (CS) in 16 culture media, and to verify which medium is most suitable for detection of the toxin. Agar plugs were extracted and they were put in KOH for 16 hours. Cercosporin concentration was determined by using a molar extinction coefficient of 23300 L mol -1. Under the conditions in which the fungi were cultured, it was not possible to induce the toxin in CZ, which reinforces the hypothesis that the toxin is only produced in planta. CS produced a high concentration of toxin in coconut water Sococo® medium with yeast extract, 177.34 nmol plug-1. Only the M1D medium did not produce the toxin. There was a difference in the production of toxin depending on the brand and concentration of PDA medium. It is concluded that the media with coconut water Sococo® are efficient in the induction of Cercosporin in vitro by CS, which are promising for research in this area. Furthermore, CZ did not produce the toxin in the studied conditions.
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spelling Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. MaydisIndução e detecção de toxina em Cercospora zeina e Cercospora sorghi f. sp. MaydisCercosporinMaize gray leaf spotZea mays L.Cercosporiose do milhoCercosporinaCercosporin is a phytotoxin with toxic activity against large number of organism, it is produced by hypha in the culture medium. This way, the objective of this study was to induce the production of Cercosporin in Cercospora zeina (CZ) and Cercospora sorghi f. sp. maydis (CS) in 16 culture media, and to verify which medium is most suitable for detection of the toxin. Agar plugs were extracted and they were put in KOH for 16 hours. Cercosporin concentration was determined by using a molar extinction coefficient of 23300 L mol -1. Under the conditions in which the fungi were cultured, it was not possible to induce the toxin in CZ, which reinforces the hypothesis that the toxin is only produced in planta. CS produced a high concentration of toxin in coconut water Sococo® medium with yeast extract, 177.34 nmol plug-1. Only the M1D medium did not produce the toxin. There was a difference in the production of toxin depending on the brand and concentration of PDA medium. It is concluded that the media with coconut water Sococo® are efficient in the induction of Cercosporin in vitro by CS, which are promising for research in this area. Furthermore, CZ did not produce the toxin in the studied conditions.A cercosporina é uma fitoxina com atividade tóxica contra um amplo espectro de organismos, produzida pelas hifas em meio de cultura. O objetivo deste estudo foi induzir a toxina em Cercospora zeina (CZ) e Cercospora sorghi f. sp. maydis (CS) em 16 meios de cultura e averiguar qual o meio mais adequado para se detectar a toxina. Discos miceliais foram imersos em KOH por 16 horas e a concentração de toxina foi calculada utilizando-se o coeficiente de 23.300 L mol -1. Nas condições em que foram submetidos os fungos não foi possível induzir a toxina em CZ, fato que reforça a hipótese de que a toxina é produzida apenas em planta. Em CS, a produção de toxina foi intensa nos meios com água de coco da marca Sococo®, principalmente quando foi adicionado extrato de leveduras, apresentando 177.34 nmol disco-1 enquanto o meio M1D não foi capaz de induzir a toxina. A diferença na produção de toxina em BDA é devida à concentração e às marcas comerciais diferentes. Conclui-se que os meios com água de coco Sococo® são eficientes na indução da Cercosporina in vitro por CS, o que constitui uma substância promissora para estudos dessa linha de pesquisa; além disto, CZ não produziu a toxina nas condições estudadas neste trabalho.Universidade Federal Rural de Pernambuco2018-02-07T12:45:37Z2018-02-07T12:45:37Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfKOSHIKUMO, E. S. M.; ALVES, E. ; VON PINHO, R. G. Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis. Revista Brasileira de Ciências Agrárias (Agrária), Recife, v. 9, n. 1, p. 31-35, 2014.http://repositorio.ufla.br/jspui/handle/1/28548Revista Brasileira de Ciências Agrárias (Agrária)reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessKoshikumo, Erika Sayuri ManetiAlves, EduardoVon Pinho, Renzo Garciaeng2023-05-26T19:52:48Zoai:localhost:1/28548Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T19:52:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
Indução e detecção de toxina em Cercospora zeina e Cercospora sorghi f. sp. Maydis
title Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
spellingShingle Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
Koshikumo, Erika Sayuri Maneti
Cercosporin
Maize gray leaf spot
Zea mays L.
Cercosporiose do milho
Cercosporina
title_short Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
title_full Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
title_fullStr Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
title_full_unstemmed Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
title_sort Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis
author Koshikumo, Erika Sayuri Maneti
author_facet Koshikumo, Erika Sayuri Maneti
Alves, Eduardo
Von Pinho, Renzo Garcia
author_role author
author2 Alves, Eduardo
Von Pinho, Renzo Garcia
author2_role author
author
dc.contributor.author.fl_str_mv Koshikumo, Erika Sayuri Maneti
Alves, Eduardo
Von Pinho, Renzo Garcia
dc.subject.por.fl_str_mv Cercosporin
Maize gray leaf spot
Zea mays L.
Cercosporiose do milho
Cercosporina
topic Cercosporin
Maize gray leaf spot
Zea mays L.
Cercosporiose do milho
Cercosporina
description Cercosporin is a phytotoxin with toxic activity against large number of organism, it is produced by hypha in the culture medium. This way, the objective of this study was to induce the production of Cercosporin in Cercospora zeina (CZ) and Cercospora sorghi f. sp. maydis (CS) in 16 culture media, and to verify which medium is most suitable for detection of the toxin. Agar plugs were extracted and they were put in KOH for 16 hours. Cercosporin concentration was determined by using a molar extinction coefficient of 23300 L mol -1. Under the conditions in which the fungi were cultured, it was not possible to induce the toxin in CZ, which reinforces the hypothesis that the toxin is only produced in planta. CS produced a high concentration of toxin in coconut water Sococo® medium with yeast extract, 177.34 nmol plug-1. Only the M1D medium did not produce the toxin. There was a difference in the production of toxin depending on the brand and concentration of PDA medium. It is concluded that the media with coconut water Sococo® are efficient in the induction of Cercosporin in vitro by CS, which are promising for research in this area. Furthermore, CZ did not produce the toxin in the studied conditions.
publishDate 2014
dc.date.none.fl_str_mv 2014
2018-02-07T12:45:37Z
2018-02-07T12:45:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv KOSHIKUMO, E. S. M.; ALVES, E. ; VON PINHO, R. G. Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis. Revista Brasileira de Ciências Agrárias (Agrária), Recife, v. 9, n. 1, p. 31-35, 2014.
http://repositorio.ufla.br/jspui/handle/1/28548
identifier_str_mv KOSHIKUMO, E. S. M.; ALVES, E. ; VON PINHO, R. G. Induction and detection of toxin in Cercospora zeina and Cercospora sorghi f. sp. Maydis. Revista Brasileira de Ciências Agrárias (Agrária), Recife, v. 9, n. 1, p. 31-35, 2014.
url http://repositorio.ufla.br/jspui/handle/1/28548
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
publisher.none.fl_str_mv Universidade Federal Rural de Pernambuco
dc.source.none.fl_str_mv Revista Brasileira de Ciências Agrárias (Agrária)
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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