Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFMA |
Texto Completo: | https://tedebc.ufma.br/jspui/handle/tede/tede/3310 |
Resumo: | Studies about vectorial capacity and competence for the transmission of certain parasites require laboratory colonization of their biological vectors. The sand fly Lutzomyia longipalpis is incriminated as the main vector of Leishmania (Leishmania) infantum, but it has been shown to be permissive for several Leishmania species that cause american cutaneous leishmaniasis (ACL). Therefore, we demonstrate the competence of Lu. longipalpis in transmitting Leishmania (Leishmania) amazonensis in mice, causing clinical manifestation of ACL in these animals. Initially, we stablished a colony of Lu. longipalpis from females sampled in the municipality of Raposa, MA, using CDC light traps. The colony produced ten generations (F10) from the parental generation (P). The first four generations showed the highest productivity. The whole life cycle lasted on average 28 + 0.5 days at 27°C and 80% relative humidity. Nonviable eggs represented more than 50% of the total number of eggs produced by the engorged females, while pupae had a mortality rate of only 2%. In the tests for vectorial capacity and competence 291 uninfected females of Lu. longipalpis of generation F1, F2 and F3 performed a first blood feeding on mice infected with Le. amazonensis (strain IFLA / BR / 1968 / PH8). After blood-feeding, 52.6% of the females were positive for Leishmania DNA. Of these, 17 (of which 58,8% were positive for Leishmania by dissection of the intestine) performed a second feeding on uninfected mice. At 27 days post-infection, one mouse presented leishmaniotic lesions. The limiting dilution test and immunohistopathological analyses confirmed the occurrence of Leishmania promastigote and amastigote forms, respectively, in the skin of the animal. Parasite DNA was also detected in lesions on paws and inguinal lymph node. DNA sequencing and analysis confirmed the Leishmania species infecting both insects and mice. Our results confirm that Lu. longipalpis can sustain infection and experimentally transmit Le. amazonensis to rodents, inducing leishmaniastic lesions. From the epidemiological perspective of the urban ACL, the successful transmission demonstrated supports the role of Lu. longipalpis as a vector of Le. amazonensis in the studied region and possibly in others throughout Brazil and the New World, where both sand fly and parasite are widespread. |
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REBÊLO, José Manuel Macáriohttp://lattes.cnpq.br/1026308157852394PEREIRA, Silma Regina Ferreirahttp://lattes.cnpq.br/8510523766431676REBÊLO, José Manuel Macáriohttp://lattes.cnpq.br/1026308157852394COSTA JUNIOR, Lívio Martinshttp://lattes.cnpq.br/6651961821189728SILVA, Ana Lúcia Abreuhttp://lattes.cnpq.br/8288733951324759ANDRADE FILHO, José Dilermandohttp://lattes.cnpq.br/7374065738760207PEREIRA FILHO, Adalberto Alveshttp://lattes.cnpq.br/9165045834831122http://lattes.cnpq.br/0975041928871117SILVA, Rosa Cristina Ribeiro da2021-05-18T14:09:21Z2021-04-13SILVA, Rosa Cristina Ribeiro da. Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi. 2021. 111 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.https://tedebc.ufma.br/jspui/handle/tede/tede/3310Studies about vectorial capacity and competence for the transmission of certain parasites require laboratory colonization of their biological vectors. The sand fly Lutzomyia longipalpis is incriminated as the main vector of Leishmania (Leishmania) infantum, but it has been shown to be permissive for several Leishmania species that cause american cutaneous leishmaniasis (ACL). Therefore, we demonstrate the competence of Lu. longipalpis in transmitting Leishmania (Leishmania) amazonensis in mice, causing clinical manifestation of ACL in these animals. Initially, we stablished a colony of Lu. longipalpis from females sampled in the municipality of Raposa, MA, using CDC light traps. The colony produced ten generations (F10) from the parental generation (P). The first four generations showed the highest productivity. The whole life cycle lasted on average 28 + 0.5 days at 27°C and 80% relative humidity. Nonviable eggs represented more than 50% of the total number of eggs produced by the engorged females, while pupae had a mortality rate of only 2%. In the tests for vectorial capacity and competence 291 uninfected females of Lu. longipalpis of generation F1, F2 and F3 performed a first blood feeding on mice infected with Le. amazonensis (strain IFLA / BR / 1968 / PH8). After blood-feeding, 52.6% of the females were positive for Leishmania DNA. Of these, 17 (of which 58,8% were positive for Leishmania by dissection of the intestine) performed a second feeding on uninfected mice. At 27 days post-infection, one mouse presented leishmaniotic lesions. The limiting dilution test and immunohistopathological analyses confirmed the occurrence of Leishmania promastigote and amastigote forms, respectively, in the skin of the animal. Parasite DNA was also detected in lesions on paws and inguinal lymph node. DNA sequencing and analysis confirmed the Leishmania species infecting both insects and mice. Our results confirm that Lu. longipalpis can sustain infection and experimentally transmit Le. amazonensis to rodents, inducing leishmaniastic lesions. From the epidemiological perspective of the urban ACL, the successful transmission demonstrated supports the role of Lu. longipalpis as a vector of Le. amazonensis in the studied region and possibly in others throughout Brazil and the New World, where both sand fly and parasite are widespread.Estudos de capacidade e competência vetorial requerem a disponibilidade de colônias de insetos vetores de doenças em condições laboratoriais. O táxon Lutzomyia longipalpis é incriminado como vetor natural de Leishmania (Leishmania) infantum, porém tem se demonstrado permissivo para várias espécies de Leishmania que causam leishmaniose tegumentar americana (LTA). Por esta razão, o trabalho teve como objetivo demonstrar a competência de Lu. longipalpis em transmitir Leishmania (Leishmania) amazonensis em camundongo causando manifestação clínica de LTA no animal. Inicialmente, foi estabelecida uma colônia de Lu. longipalpis a partir de fêmeas capturadas no município da Raposa, MA, utilizando-se armadilha luminosa do tipo CDC. A colônia produziu dez gerações (F10) a partir da geração parental (P). A maior produtividade da colônia foi nas quatro primeiras gerações. O ciclo de vida completo durou, em média, 28 + 0,5 dias, a 27°C e umidade relativa de 80%. Os ovos inviáveis representaram mais de 50% do total de ovos produzidos pelas fêmeas ingurgitadas, enquanto as pupas apresentaram índice de mortalidade de apenas 2%. Nos ensaios de capacidade e competência vetorial, 291 fêmeas de Lu. longipalpis de geração F1, F2 e F3 (livre de infecção de Leishmania) realizaram um primeiro repasto sanguíneo em camundongos infectados com Le. amazonensis (linhagem IFLA/BR/1968/PH8). Após a alimentação, 52,6% positivaram DNA de Leishmania. Destes, 17 (dos quais 58,8% foram positivos para Leishmania por dissecação do intestino) efetuaram um segundo repasto sanguíneo em camundongos não infectados. Após 27 dias de infecção, um dos camundongos apresentou um edema no membro anterior direito característico de lesão leishmaniótica. O teste de diluição limitante e as análises imunohistopatológicas confirmaram a ocorrência de formas promastigotas e amastigostas de Leishmania, respectivamente, na lesão do membro anterior do animal. Também foi detectado DNA do parasita em lesões do membro anterior esquerdo e linfonodo poplíteo. O sequenciamento do DNA confirmou a espécie Leishmania tanto em insetos como no camundongo. Os resultados confirmam a capacidade de Lu. longipalpis sustentar a infecção e transmitir experimentalmente Le. amazonensis a roedores, induzindo lesões leishmanióticas. Do ponto de vista epidemiológico da LTA urbana, onde tanto Lu. longipalpis como Le. amozonensis coabitam extensivamente pelo Novo Mundo, inclusive em várias regiões do Brasil, a suscetibilidade do teste de transmissão confirma a possibilidade do papel vetorial deste flebotomíneo para transmitir Le. amazonensis na região estudada.Submitted by Sheila MONTEIRO (sheila.monteiro@ufma.br) on 2021-05-18T14:09:21Z No. of bitstreams: 1 ROSA-SILVA.pdf: 4540270 bytes, checksum: 76da788e7af61d8d5e31361fb06975a2 (MD5)Made available in DSpace on 2021-05-18T14:09:21Z (GMT). No. of bitstreams: 1 ROSA-SILVA.pdf: 4540270 bytes, checksum: 76da788e7af61d8d5e31361fb06975a2 (MD5) Previous issue date: 2021-04-13Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão - FAPEMAapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBSUFMABrasilDEPARTAMENTO DE BIOLOGIA/CCBSVector permissivoTransmissão experimentalColônia de flebotomíneosLeishmaniose tegumentarInteração vetor-parasitoPermissive vectorExperimental transmissionSand fly colonyTegumentary leishmaniasisVector-parasite interactionDoenças Infecciosas e ParasitáriasEstudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensiStudy of the capacity and competence of Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) as a vector for Leishmania (Leishmania) amazonensiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALROSA-SILVA.pdfROSA-SILVA.pdfapplication/pdf4540270http://tedebc.ufma.br:8080/bitstream/tede/3310/2/ROSA-SILVA.pdf76da788e7af61d8d5e31361fb06975a2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/3310/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/33102021-05-18 11:50:03.459oai:tede2:tede/3310IExJQ0VOw4dBIERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBCgpDb20gYSBhcHJlc2VudGHDp8OjbyBkZXN0YSBsaWNlbsOnYSxvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvciBjb25jZWRlIMOgIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIE1hcmFuaMOjbyAoVUZNQSkgbyBkaXJlaXRvIG7Do28tZXhjbHVzaXZvIGRlIHJlcHJvZHV6aXIsIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IGRpc3RyaWJ1aXIgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyw7RuaWNvIGUgZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIGZvcm1hdG9zIMOhdWRpbyBvdSB2w61kZW8uCgpWb2PDqiBjb25jb3JkYSBxdWUgYSBVRk1BIHBvZGUsIHNlbSBhbHRlcmFyIG8gY29udGXDumRvLCB0cmFuc3BvciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVGTUEgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRlIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gcGFyYSBmaW5zIGRlIHNlZ3VyYW7Dp2EsIGJhY2stdXAgZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIMOpIG9yaWdpbmFsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBWb2PDqiB0YW1iw6ltIGRlY2xhcmEgcXVlIG8gZGVww7NzaXRvIGRhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gbsOjbywgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiBkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVUZNQSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBvcmEgZGVwb3NpdGFkYS4KCkNBU08gQSBURVNFIE9VIERJU1NFUlRBw4fDg08gT1JBIERFUE9TSVRBREEgVEVOSEEgU0lETyBSRVNVTFRBRE8gREUgVU0gUEFUUk9Dw41OSU8gT1UgQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRk1BLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyBUQU1Cw4lNIEFTIERFTUFJUyBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUE9SIENPTlRSQVRPIE9VIEFDT1JETy4KCkEgVUZNQSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIG91IG8ocykgbm9tZShzKSBkbyhzKSBkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcyBjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKRGVjbGFyYSB0YW1iw6ltIHF1ZSB0b2RhcyBhcyBhZmlsaWHDp8O1ZXMgY29ycG9yYXRpdmFzIG91IGluc3RpdHVjaW9uYWlzIGUgdG9kYXMgYXMgZm9udGVzIGRlIGFwb2lvIGZpbmFuY2Vpcm8gYW8gdHJhYmFsaG8gZXN0w6NvIGRldmlkYW1lbnRlIGNpdGFkYXMgb3UgbWVuY2lvbmFkYXMgZSBjZXJ0aWZpY2EgcXVlIG7Do28gaMOhIG5lbmh1bSBpbnRlcmVzc2UgY29tZXJjaWFsIG91IGFzc29jaWF0aXZvIHF1ZSByZXByZXNlbnRlIGNvbmZsaXRvIGRlIGludGVyZXNzZSBlbSBjb25leMOjbyBjb20gbyB0cmFiYWxobyBzdWJtZXRpZG8uCgoKCgoKCgo=Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312021-05-18T14:50:03Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
dc.title.por.fl_str_mv |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
dc.title.alternative.eng.fl_str_mv |
Study of the capacity and competence of Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) as a vector for Leishmania (Leishmania) amazonensi |
title |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
spellingShingle |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi SILVA, Rosa Cristina Ribeiro da Vector permissivo Transmissão experimental Colônia de flebotomíneos Leishmaniose tegumentar Interação vetor-parasito Permissive vector Experimental transmission Sand fly colony Tegumentary leishmaniasis Vector-parasite interaction Doenças Infecciosas e Parasitárias |
title_short |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
title_full |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
title_fullStr |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
title_full_unstemmed |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
title_sort |
Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi |
author |
SILVA, Rosa Cristina Ribeiro da |
author_facet |
SILVA, Rosa Cristina Ribeiro da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
REBÊLO, José Manuel Macário |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1026308157852394 |
dc.contributor.advisor-co1.fl_str_mv |
PEREIRA, Silma Regina Ferreira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8510523766431676 |
dc.contributor.referee1.fl_str_mv |
REBÊLO, José Manuel Macário |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1026308157852394 |
dc.contributor.referee2.fl_str_mv |
COSTA JUNIOR, Lívio Martins |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6651961821189728 |
dc.contributor.referee3.fl_str_mv |
SILVA, Ana Lúcia Abreu |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8288733951324759 |
dc.contributor.referee4.fl_str_mv |
ANDRADE FILHO, José Dilermando |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/7374065738760207 |
dc.contributor.referee5.fl_str_mv |
PEREIRA FILHO, Adalberto Alves |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/9165045834831122 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0975041928871117 |
dc.contributor.author.fl_str_mv |
SILVA, Rosa Cristina Ribeiro da |
contributor_str_mv |
REBÊLO, José Manuel Macário PEREIRA, Silma Regina Ferreira REBÊLO, José Manuel Macário COSTA JUNIOR, Lívio Martins SILVA, Ana Lúcia Abreu ANDRADE FILHO, José Dilermando PEREIRA FILHO, Adalberto Alves |
dc.subject.por.fl_str_mv |
Vector permissivo Transmissão experimental Colônia de flebotomíneos Leishmaniose tegumentar Interação vetor-parasito |
topic |
Vector permissivo Transmissão experimental Colônia de flebotomíneos Leishmaniose tegumentar Interação vetor-parasito Permissive vector Experimental transmission Sand fly colony Tegumentary leishmaniasis Vector-parasite interaction Doenças Infecciosas e Parasitárias |
dc.subject.eng.fl_str_mv |
Permissive vector Experimental transmission Sand fly colony Tegumentary leishmaniasis Vector-parasite interaction |
dc.subject.cnpq.fl_str_mv |
Doenças Infecciosas e Parasitárias |
description |
Studies about vectorial capacity and competence for the transmission of certain parasites require laboratory colonization of their biological vectors. The sand fly Lutzomyia longipalpis is incriminated as the main vector of Leishmania (Leishmania) infantum, but it has been shown to be permissive for several Leishmania species that cause american cutaneous leishmaniasis (ACL). Therefore, we demonstrate the competence of Lu. longipalpis in transmitting Leishmania (Leishmania) amazonensis in mice, causing clinical manifestation of ACL in these animals. Initially, we stablished a colony of Lu. longipalpis from females sampled in the municipality of Raposa, MA, using CDC light traps. The colony produced ten generations (F10) from the parental generation (P). The first four generations showed the highest productivity. The whole life cycle lasted on average 28 + 0.5 days at 27°C and 80% relative humidity. Nonviable eggs represented more than 50% of the total number of eggs produced by the engorged females, while pupae had a mortality rate of only 2%. In the tests for vectorial capacity and competence 291 uninfected females of Lu. longipalpis of generation F1, F2 and F3 performed a first blood feeding on mice infected with Le. amazonensis (strain IFLA / BR / 1968 / PH8). After blood-feeding, 52.6% of the females were positive for Leishmania DNA. Of these, 17 (of which 58,8% were positive for Leishmania by dissection of the intestine) performed a second feeding on uninfected mice. At 27 days post-infection, one mouse presented leishmaniotic lesions. The limiting dilution test and immunohistopathological analyses confirmed the occurrence of Leishmania promastigote and amastigote forms, respectively, in the skin of the animal. Parasite DNA was also detected in lesions on paws and inguinal lymph node. DNA sequencing and analysis confirmed the Leishmania species infecting both insects and mice. Our results confirm that Lu. longipalpis can sustain infection and experimentally transmit Le. amazonensis to rodents, inducing leishmaniastic lesions. From the epidemiological perspective of the urban ACL, the successful transmission demonstrated supports the role of Lu. longipalpis as a vector of Le. amazonensis in the studied region and possibly in others throughout Brazil and the New World, where both sand fly and parasite are widespread. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-18T14:09:21Z |
dc.date.issued.fl_str_mv |
2021-04-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Rosa Cristina Ribeiro da. Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi. 2021. 111 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021. |
dc.identifier.uri.fl_str_mv |
https://tedebc.ufma.br/jspui/handle/tede/tede/3310 |
identifier_str_mv |
SILVA, Rosa Cristina Ribeiro da. Estudo da capacidade e competência de Lutzomyia longipalpis (DIPTERA: PSYCHODIDAE) como vetor de Leishmania (Leishmania) amazonensi. 2021. 111 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021. |
url |
https://tedebc.ufma.br/jspui/handle/tede/tede/3310 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.publisher.program.fl_str_mv |
PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBS |
dc.publisher.initials.fl_str_mv |
UFMA |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE BIOLOGIA/CCBS |
publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFMA instname:Universidade Federal do Maranhão (UFMA) instacron:UFMA |
instname_str |
Universidade Federal do Maranhão (UFMA) |
instacron_str |
UFMA |
institution |
UFMA |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFMA |
collection |
Biblioteca Digital de Teses e Dissertações da UFMA |
bitstream.url.fl_str_mv |
http://tedebc.ufma.br:8080/bitstream/tede/3310/2/ROSA-SILVA.pdf http://tedebc.ufma.br:8080/bitstream/tede/3310/1/license.txt |
bitstream.checksum.fl_str_mv |
76da788e7af61d8d5e31361fb06975a2 97eeade1fce43278e63fe063657f8083 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA) |
repository.mail.fl_str_mv |
repositorio@ufma.br||repositorio@ufma.br |
_version_ |
1800303802362888192 |