Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies

Detalhes bibliográficos
Autor(a) principal: Abreu, Iliano V. Coutinho
Data de Publicação: 2020
Outros Autores: Oristian, James, Castro, Waldionê de, Wilson, Timothy R., Meneses, Claudio, Soares, Rodrigo P., Borges, Valéria de Matos, Descoteaux, Albert, Kamhawi, Shaden, Valenzuela, Jesus G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44044
Resumo: Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases. A.D. holds the Canada Research Chair on the Biology of Intracellular Parasitism
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spelling Abreu, Iliano V. CoutinhoOristian, JamesCastro, Waldionê deWilson, Timothy R.Meneses, ClaudioSoares, Rodrigo P.Borges, Valéria de MatosDescoteaux, AlbertKamhawi, ShadenValenzuela, Jesus G.2020-10-19T11:05:55Z2020-10-19T11:05:55Z2020ABREU, Iliano V. Coutinho et al. Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies. mSphere, v. 5, n. 5, p. e00594-20, 2020.s. dhttps://www.arca.fiocruz.br/handle/icict/4404410.1128/mSphere.00594-20Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases. A.D. holds the Canada Research Chair on the Biology of Intracellular ParasitismNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.Fundação Oswaldo Cruz. Instituto René rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Salvador, BA, Brasil.Centre Armand-Frappier Santé Biotechnologie. Laval, Quebec, Canada.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.The major surface lipophosphoglycan (LPG) of Leishmania parasites is critical to vector competence in restrictive sand fly vectors in mediating Leishmania attachment to the midgut epithelium, considered essential to parasite survival and development. However, the relevance of LPG for sand flies that harbor multiple species of Leishmania remains elusive. We tested binding of Leishmania infantum wildtype (WT), LPG-defective (Δlpg1 mutants), and add-back (Δlpg1 LPG1) lines to sand fly midguts in vitro and their survival in Lutzomyia longipalpis sand flies in vivo. Le. infantum WT parasites attached to the Lu. longipalpis midgut in vitro, with late-stage parasites binding to midguts in significantly higher numbers than were seen with early-stage promastigotes. Δlpg1 mutants did not bind to Lu. longipalpis midguts, and this was rescued in the Δlpg1 LPG1 lines, indicating that midgut binding is mediated by LPG. When Lu. longipalpis sand flies were infected with the Le. infantum WT or Le. infantum Δlpg1 or Le. infantum Δlpg1 LPG1 line of the BH46 or BA262 strains, the BH46 Δlpg1 mutant, but not the BA262 Δlpg1 mutant, survived and grew to numbers similar to those seen with the WT and Δlpg1 LPG1 lines. Exposure of BH46 and BA262 Δlpg1 mutants to blood-engorged midgut extracts led to mortality of the BA262 Δlpg1 but not the BH46 Δlpg1 parasites. These findings suggest that Le. infantum LPG protects parasites on a strain-specific basis early in infection, likely against toxic components of blood digestion, but that it is not necessary to prevent Le. infantum evacuation along with the feces in the permissive vector Lu. longipalpis.engAmerican Society for MicrobiologyLeishmaniaParasitaSobrevivênciaFlebotomosIntestinoSalivaLeishmania infantumLPGLeishmaniaParasite bindingParasite survivalSand flySand fly midgutVector competenceLeishmania infantumBinding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Fliesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
title Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
spellingShingle Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
Abreu, Iliano V. Coutinho
Leishmania
Parasita
Sobrevivência
Flebotomos
Intestino
Saliva
Leishmania infantum
LPG
Leishmania
Parasite binding
Parasite survival
Sand fly
Sand fly midgut
Vector competence
Leishmania infantum
title_short Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
title_full Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
title_fullStr Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
title_full_unstemmed Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
title_sort Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies
author Abreu, Iliano V. Coutinho
author_facet Abreu, Iliano V. Coutinho
Oristian, James
Castro, Waldionê de
Wilson, Timothy R.
Meneses, Claudio
Soares, Rodrigo P.
Borges, Valéria de Matos
Descoteaux, Albert
Kamhawi, Shaden
Valenzuela, Jesus G.
author_role author
author2 Oristian, James
Castro, Waldionê de
Wilson, Timothy R.
Meneses, Claudio
Soares, Rodrigo P.
Borges, Valéria de Matos
Descoteaux, Albert
Kamhawi, Shaden
Valenzuela, Jesus G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abreu, Iliano V. Coutinho
Oristian, James
Castro, Waldionê de
Wilson, Timothy R.
Meneses, Claudio
Soares, Rodrigo P.
Borges, Valéria de Matos
Descoteaux, Albert
Kamhawi, Shaden
Valenzuela, Jesus G.
dc.subject.other.pt_BR.fl_str_mv Leishmania
Parasita
Sobrevivência
Flebotomos
Intestino
Saliva
Leishmania infantum
topic Leishmania
Parasita
Sobrevivência
Flebotomos
Intestino
Saliva
Leishmania infantum
LPG
Leishmania
Parasite binding
Parasite survival
Sand fly
Sand fly midgut
Vector competence
Leishmania infantum
dc.subject.en.pt_BR.fl_str_mv LPG
Leishmania
Parasite binding
Parasite survival
Sand fly
Sand fly midgut
Vector competence
Leishmania infantum
description Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases. A.D. holds the Canada Research Chair on the Biology of Intracellular Parasitism
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-19T11:05:55Z
dc.date.available.fl_str_mv 2020-10-19T11:05:55Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABREU, Iliano V. Coutinho et al. Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies. mSphere, v. 5, n. 5, p. e00594-20, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44044
dc.identifier.issn.pt_BR.fl_str_mv s. d
dc.identifier.doi.none.fl_str_mv 10.1128/mSphere.00594-20
identifier_str_mv ABREU, Iliano V. Coutinho et al. Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies. mSphere, v. 5, n. 5, p. e00594-20, 2020.
s. d
10.1128/mSphere.00594-20
url https://www.arca.fiocruz.br/handle/icict/44044
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44044/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44044/2/Ilianano%20V%2c%20%20Binding.pdf
https://www.arca.fiocruz.br/bitstream/icict/44044/3/Ilianano%20V%2c%20%20Binding.pdf.txt
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